YWTD domain of low-density lipoprotein receptor

The low-density lipoprotein receptor (LDLR) regulates cholesterol homeostasis in mammalian cells. LDLR binds cholesterol-carrying LDL, associates with clathrin-coated pits, and is internalized into acidic endosomes where it separates from its ligand. The ligand is degraded in lysosomes, while the receptor returns to the cell surface.[1] The LDLR has several domains. The ligand-binding domain contains seven LDL receptor class A repeats, each with three disulphide bonds and a coordinated Ca2+ ion. The second conserved region contains two EGF repeats, followed by six YWTD or LDL receptor class B repeats and another EGF repeat.[2] This conserved region is critical for ligand release and recycling of the receptor.[3]

The structure of the six YWTD repeats of LDL receptor have been solved.[4] The six YWTD repeats together fold into a six-bladed beta-propeller. Each blade of the propeller consists of four antiparallel beta-strands; the innermost strand of each blade is labeled 1 and the outermost strand, 4. The sequence repeats are offset with respect to the blades of the propeller, such that any given 40-residue YWTD repeat spans strands 24 of one propeller blade and strand 1 of the subsequent blade. This offset ensures circularization of the propeller because the last strand of the final sequence repeat acts as an innermost strand 1 of the blade that harbors strands 24 from the first sequence repeat. The repeat is found in a variety of proteins that include, vitellogenin receptor from Drosophila melanogaster, low-density lipoprotein (LDL) receptor,[5] preproepidermal growth factor, and nidogen (entactin).

References

  1. ↑ Brown MS, Goldstein JL (1986). "A receptor-mediated pathway for cholesterol homeostasis". Science. 232 (4746): 34–47. PMID 3513311. doi:10.1126/science.3513311.
  2. ↑ Springer TA (1998). "An extracellular beta-propeller module predicted in lipoprotein and scavenger receptors, tyrosine kinases, epidermal growth factor precursor, and extracellular matrix components". J. Mol. Biol. 283 (4): 837–862. PMID 9790844. doi:10.1006/jmbi.1998.2115.
  3. ↑ Russell DW, Brown MS, Goldstein JL, Davis CG, Sudhof TC, Anderson RG (1987). "Acid-dependent ligand dissociation and recycling of LDL receptor mediated by growth factor homology region". Nature. 326 (6115): 760–765. PMID 3494949. doi:10.1038/326760a0.
  4. ↑ Eck MJ, Springer TA, Blacklow SC, Takagi J, Jeon H, Meng W (2001). "Implications for familial hypercholesterolemia from the structure of the LDL receptor YWTD-EGF domain pair". Nat. Struct. Biol. 8 (6): 499–504. PMID 11373616. doi:10.1038/88556.
  5. ↑ Russell DW, Schneider WJ, Yamamoto T, Brown MS, Goldstein JL, Davis CG, Casey ML (1984). "The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA". Cell. 39 (1): 27–38. PMID 6091915. doi:10.1016/0092-8674(84)90188-0.

This article incorporates text from the public domain Pfam and InterPro IPR000033

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