Weak affinity chromatography

Weak affinity chromatography[1] (WAC) is a robust and highly reproducible technology for affinity screening in drug development.[2][3] WAC is an affinity-based liquid chromatographic technique that separates chemical compounds based on their different weak affinities to an immobilized target.

Overview

The higher affinity a compound has towards the target, the longer it remains in the separation unit, and this will be expressed as a longer retention time. The affinity measure and ranking of affinity can be achieved by processing the obtained retention times of analyzed compounds.

Protein targets

The WAC technology is demonstrated against a number of different protein targets – proteases, kinases, chaperones and protein–protein interaction (PPI) targets.

Advantages

WAC has been shown to be more effective than established methods for fragment based screening.[3]

References

  1. ↑ Zopf, D.; S. Ohlson (1990). "Weak-affinity chromatography". Nature. 346 (6279): 87–88. ISSN 0028-0836. doi:10.1038/346087a0.
  2. ↑ Duong-Thi, M. D.; Meiby, E.; Bergström, M.; Fex, T.; Isaksson, R.; Ohlson, S. (2011). "Weak affinity chromatography as a new approach for fragment screening in drug discovery". Analytical Biochemistry. 414 (1): 138–146. PMID 21352794. doi:10.1016/j.ab.2011.02.022.
  3. 1 2 Meiby, E.; Simmonite, H.; Le Strat, L.; Davis, B.; Matassova, N.; Moore, J. D.; Mrosek, M.; Murray, J.; Hubbard, R. E.; Ohlson, S. (2013). "Fragment Screening by Weak Affinity Chromatography: Comparison with Established Techniques for Screening against HSP90". Analytical Chemistry. 85 (14): 6756–6766. PMID 23806099. doi:10.1021/ac400715t.


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