TRNA pseudouridine32 synthase

TRNA pseudouridine32 synthase
Identifiers
EC number 5.4.99.28
CAS number 430429-15-5
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

TRNA pseudouridine32 synthase (EC 5.4.99.28, RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic name tRNA-uridine32 uracil mutase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

tRNA uridine32 tRNA pseudouridine32

The dual enzyme from Escherichia coli also catalyses the formation of pseudouridine746 in 23S rRNA.

References

  1. ↑ Hoang, C.; Chen, J.; Vizthum, C.A.; Kandel, J.M.; Hamilton, C.S.; Mueller, E.G.; Ferre-D'Amare, A.R. (2006). "Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure". Mol. Cell. 24: 535–545. PMID 17188032. doi:10.1016/j.molcel.2006.09.017.
  2. ↑ Spedaliere, C.J.; Hamilton, C.S.; Mueller, E.G. (2000). "Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues". Biochemistry. 39: 9459–9465. PMID 10924141. doi:10.1021/bi001079n.
  3. ↑ Raychaudhuri, S.; Niu, L.; Conrad, J.; Lane, B.G.; Ofengand, J. (1999). "Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA". J. Biol. Chem. 274: 18880–18886. PMID 10383384. doi:10.1074/jbc.274.27.18880.
  4. ↑ Ramamurthy, V.; Swann, S.L.; Spedaliere, C.J.; Mueller, E.G. (1999). "Role of cysteine residues in pseudouridine synthases of different families". Biochemistry. 38: 13106–13111. PMID 10529181. doi:10.1021/bi9913911.
  5. ↑ Wrzesinski, J.; Nurse, K.; Bakin, A.; Lane, B.G.; Ofengand, J. (1995). "A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for Ψ746 in 23S RNA is also specific for Ψ32 in tRNAPhe". RNA. 1: 437–448. PMC 1482406 Freely accessible. PMID 7493321.
  6. ↑ Behm-Ansmant, I.; Grosjean, H.; Massenet, S.; Motorin, Y.; Branlant, C. (2004). "Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae". J. Biol. Chem. 279: 52998–53006. PMID 15466869. doi:10.1074/jbc.m409581200.
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