Replication protein A2

RPA2

Available structures

Ortholog search: PDBe RCSB

List of PDB id codes
1DPU, 1L1O, 1QUQ, 1Z1D, 2PI2, 2PQA, 2Z6K, 3KDF, 4MQV, 4OU0

Identifiers

Aliases

RPA2, REPA2, RP-A p32, RP-A p34, RPA32, replication protein A2

External IDs

MGI: 1339939 HomoloGene: 37712 GeneCards: RPA2

Gene location (Human)

Chr.	Chromosome 1 (human)^[1]

Band	No data available	Start	27,891,524 bp^[1]
Band	No data available	End	27,914,746 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 4 (mouse)^[2]

Band	No data available	Start	132,768,332 bp^[2]
Band	No data available	End	132,778,752 bp^[2]

RNA expression pattern

More reference expression data

Gene ontology
Molecular function	• DNA binding • protein N-terminus binding • single-stranded DNA binding • damaged DNA binding • protein binding • enzyme binding • protein phosphatase binding • ubiquitin protein ligase binding • G-rich strand telomeric DNA binding
Cellular component	• site of double-strand break • PML body • nucleoplasm • chromosome, telomeric region • DNA replication factor A complex • chromatin • nucleus • nuclear chromosome, telomeric region • nuclear body
Biological process	• nucleotide-excision repair, DNA gap filling • DNA recombination • interstrand cross-link repair • regulation of double-strand break repair via homologous recombination • error-free translesion synthesis • G1/S transition of mitotic cell cycle • error-prone translesion synthesis • cellular response to DNA damage stimulus • DNA replication • mitotic G1 DNA damage checkpoint • regulation of DNA damage checkpoint • regulation of cellular response to heat • mismatch repair • translesion synthesis • transcription-coupled nucleotide-excision repair • nucleotide-excision repair, DNA incision • DNA damage response, detection of DNA damage • base-excision repair • nucleotide-excision repair • nucleotide-excision repair, preincision complex stabilization • regulation of signal transduction by p53 class mediator • DNA repair • double-strand break repair via homologous recombination • nucleotide-excision repair, preincision complex assembly • nucleotide-excision repair, DNA incision, 5'-to lesion • nucleotide-excision repair, DNA incision, 3'-to lesion • protein localization to chromosome • telomere maintenance • telomere maintenance via semi-conservative replication
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


6118


19891

Ensembl


ENSG00000117748


ENSMUSG00000028884

UniProt


P15927


Q62193

RefSeq (mRNA)


NM_001286076 NM_001297558 NM_002946


NM_011284

RefSeq (protein)


NP_001273005 NP_001284487 NP_002937


n/a

Location (UCSC)

Chr 1: 27.89 – 27.91 Mb

Chr 1: 132.77 – 132.78 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Replication protein A 32 kDa subunit is a protein that in humans is encoded by the RPA2 gene.^[5]^[6]

Interactions

RPA2 has been shown to interact with:

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000117748 - Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000028884 - Ensembl, May 2017
↑ "Human PubMed Reference:".
↑ "Mouse PubMed Reference:".
↑ Umbricht CB, Erdile LF, Jabs EW, Kelly TJ (Mar 1993). "Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A". The Journal of Biological Chemistry. 268 (9): 6131–8. PMID 8454588.
↑ "Entrez Gene: RPA2 Replication protein A2, 32kDa".
↑ Otterlei M, Warbrick E, Nagelhus TA, Haug T, Slupphaug G, Akbari M, Aas PA, Steinsbekk K, Bakke O, Krokan HE (Jul 1999). "Post-replicative base excision repair in replication foci". The EMBO Journal. 18 (13): 3834–44. PMC 1171460 . PMID 10393198. doi:10.1093/emboj/18.13.3834.
1 2 3 Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y (Mar 1999). "Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes". The EMBO Journal. 18 (5): 1397–406. PMC 1171229 . PMID 10064605. doi:10.1093/emboj/18.5.1397.
↑ Sukhodolets KE, Hickman AB, Agarwal SK, Sukhodolets MV, Obungu VH, Novotny EA, Crabtree JS, Chandrasekharappa SC, Collins FS, Spiegel AM, Burns AL, Marx SJ (Jan 2003). "The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene". Molecular and Cellular Biology. 23 (2): 493–509. PMC 151531 . PMID 12509449. doi:10.1128/mcb.23.2.493-509.2003.
1 2 Bochkareva E, Korolev S, Lees-Miller SP, Bochkarev A (Apr 2002). "Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA". The EMBO Journal. 21 (7): 1855–63. PMC 125950 . PMID 11927569. doi:10.1093/emboj/21.7.1855.
↑ Bochkareva E, Frappier L, Edwards AM, Bochkarev A (Feb 1998). "The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain". The Journal of Biological Chemistry. 273 (7): 3932–6. PMID 9461578. doi:10.1074/jbc.273.7.3932.
↑ Kim J, Kim D, Chung J (2000). "Replication protein a 32 kDa subunit (RPA p32) binds the SH2 domain of STAT3 and regulates its transcriptional activity". Cell Biology International. 24 (7): 467–73. PMID 10875894. doi:10.1006/cbir.2000.0525.
↑ Yoo E, Kim BU, Lee SY, Cho CH, Chung JH, Lee CH (Aug 2005). "53BP1 is associated with replication protein A and is required for RPA2 hyperphosphorylation following DNA damage". Oncogene. 24 (35): 5423–30. PMID 15856006. doi:10.1038/sj.onc.1208710.
↑ Nagelhus TA, Haug T, Singh KK, Keshav KF, Skorpen F, Otterlei M, Bharati S, Lindmo T, Benichou S, Benarous R, Krokan HE (Mar 1997). "A sequence in the N-terminal region of human uracil-DNA glycosylase with homology to XPA interacts with the C-terminal part of the 34-kDa subunit of replication protein A". The Journal of Biological Chemistry. 272 (10): 6561–6. PMID 9045683. doi:10.1074/jbc.272.10.6561.

Dutta A, Stillman B (Jun 1992). "cdc2 family kinases phosphorylate a human cell DNA replication factor, RPA, and activate DNA replication". The EMBO Journal. 11 (6): 2189–99. PMC 556686 . PMID 1318195.
Erdile LF, Wold MS, Kelly TJ (Feb 1990). "The primary structure of the 32-kDa subunit of human replication protein A". The Journal of Biological Chemistry. 265 (6): 3177–82. PMID 2406247.
Li L, Lu X, Peterson CA, Legerski RJ (Oct 1995). "An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair". Molecular and Cellular Biology. 15 (10): 5396–402. PMC 230789 . PMID 7565690. doi:10.1128/mcb.15.10.5396.
Umbricht CB, Griffin CA, Hawkins AL, Grzeschik KH, O'Connell P, Leach R, Green ED, Kelly TJ (Mar 1994). "High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3)". Genomics. 20 (2): 249–57. PMID 8020972. doi:10.1006/geno.1994.1161.
Nagelhus TA, Haug T, Singh KK, Keshav KF, Skorpen F, Otterlei M, Bharati S, Lindmo T, Benichou S, Benarous R, Krokan HE (Mar 1997). "A sequence in the N-terminal region of human uracil-DNA glycosylase with homology to XPA interacts with the C-terminal part of the 34-kDa subunit of replication protein A". The Journal of Biological Chemistry. 272 (10): 6561–6. PMID 9045683. doi:10.1074/jbc.272.10.6561.
Amacker M, Hottiger M, Mossi R, Hübscher U (Mar 1997). "HIV-1 nucleocapsid protein and replication protein A influence the strand displacement DNA synthesis of lentiviral reverse transcriptase". AIDS. 11 (4): 534–6. PMID 9084803.
Niu H, Erdjument-Bromage H, Pan ZQ, Lee SH, Tempst P, Hurwitz J (May 1997). "Mapping of amino acid residues in the p34 subunit of human single-stranded DNA-binding protein phosphorylated by DNA-dependent protein kinase and Cdc2 kinase in vitro". The Journal of Biological Chemistry. 272 (19): 12634–41. PMID 9139719. doi:10.1074/jbc.272.19.12634.
Zernik-Kobak M, Vasunia K, Connelly M, Anderson CW, Dixon K (Sep 1997). "Sites of UV-induced phosphorylation of the p34 subunit of replication protein A from HeLa cells". The Journal of Biological Chemistry. 272 (38): 23896–904. PMID 9295339. doi:10.1074/jbc.272.38.23896.
Bochkareva E, Frappier L, Edwards AM, Bochkarev A (Feb 1998). "The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain". The Journal of Biological Chemistry. 273 (7): 3932–6. PMID 9461578. doi:10.1074/jbc.273.7.3932.
Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y (Mar 1999). "Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes". The EMBO Journal. 18 (5): 1397–406. PMC 1171229 . PMID 10064605. doi:10.1093/emboj/18.5.1397.
Otterlei M, Warbrick E, Nagelhus TA, Haug T, Slupphaug G, Akbari M, Aas PA, Steinsbekk K, Bakke O, Krokan HE (Jul 1999). "Post-replicative base excision repair in replication foci". The EMBO Journal. 18 (13): 3834–44. PMC 1171460 . PMID 10393198. doi:10.1093/emboj/18.13.3834.
Bochkarev A, Bochkareva E, Frappier L, Edwards AM (Aug 1999). "The crystal structure of the complex of replication protein A subunits RPA32 and RPA14 reveals a mechanism for single-stranded DNA binding". The EMBO Journal. 18 (16): 4498–504. PMC 1171524 . PMID 10449415. doi:10.1093/emboj/18.16.4498.
Lao Y, Gomes XV, Ren Y, Taylor JS, Wold MS (Feb 2000). "Replication protein A interactions with DNA. III. Molecular basis of recognition of damaged DNA". Biochemistry. 39 (5): 850–9. PMID 10653628. doi:10.1021/bi991704s.
Costello JF, Frühwald MC, Smiraglia DJ, Rush LJ, Robertson GP, Gao X, Wright FA, Feramisco JD, Peltomäki P, Lang JC, Schuller DE, Yu L, Bloomfield CD, Caligiuri MA, Yates A, Nishikawa R, Su Huang H, Petrelli NJ, Zhang X, O'Dorisio MS, Held WA, Cavenee WK, Plass C (Feb 2000). "Aberrant CpG-island methylation has non-random and tumour-type-specific patterns". Nature Genetics. 24 (2): 132–8. PMID 10655057. doi:10.1038/72785.
Kim J, Kim D, Chung J (2000). "Replication protein a 32 kDa subunit (RPA p32) binds the SH2 domain of STAT3 and regulates its transcriptional activity". Cell Biology International. 24 (7): 467–73. PMID 10875894. doi:10.1006/cbir.2000.0525.
Cho JM, Song DJ, Bergeron J, Benlimame N, Wold MS, Alaoui-Jamali MA (Sep 2000). "RBT1, a novel transcriptional co-activator, binds the second subunit of replication protein A". Nucleic Acids Research. 28 (18): 3478–85. PMC 110737 . PMID 10982866. doi:10.1093/nar/28.18.3478.
Brush GS, Kelly TJ (Oct 2000). "Phosphorylation of the replication protein A large subunit in the Saccharomyces cerevisiae checkpoint response". Nucleic Acids Research. 28 (19): 3725–32. PMC 110765 . PMID 11000264. doi:10.1093/nar/28.19.3725.
Mer G, Bochkarev A, Gupta R, Bochkareva E, Frappier L, Ingles CJ, Edwards AM, Chazin WJ (Oct 2000). "Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA". Cell. 103 (3): 449–56. PMID 11081631. doi:10.1016/S0092-8674(00)00136-7.
Habel JE, Ohren JF, Borgstahl GE (Feb 2001). "Dynamic light-scattering analysis of full-length human RPA14/32 dimer: purification, crystallization and self-association". Acta Crystallographica Section D. 57 (Pt 2): 254–9. PMID 11173472. doi:10.1107/S0907444900015225.

PDB gallery

1dpu: SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF HUMAN RPA32 COMPLEXED WITH UNG2(73-88)

1l1o: Structure of the human Replication Protein A (RPA) trimerization core

1quq: COMPLEX OF REPLICATION PROTEIN A SUBUNITS RPA14 AND RPA32

1z1d: Structural Model for the interaction between RPA32 C-terminal domain and SV40 T antigen origin binding domain.

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Replication protein A2

Interactions

See also

References

Further reading