Prostaglandin-F synthase
| prostaglandin-F synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.1.1.188 | ||||||||
| CAS number | 55976-95-9 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / EGO | ||||||||
| |||||||||
In enzymology, a prostaglandin-F synthase (EC 1.1.1.188) is an enzyme that catalyzes the chemical reaction
- (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH + H+
Thus, the two substrates of this enzyme are (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate and NADP+, whereas its 3 products are (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate, NADPH, and H+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate:NADP+ 11-oxidoreductase. Other names in common use include prostaglandin-D2 11-reductase, reductase, 15-hydroxy-11-oxoprostaglandin, PGD2 11-ketoreductase, PGF2α synthetase, prostaglandin 11-ketoreductase, prostaglandin D2-ketoreductase, prostaglandin F synthase, prostaglandin F synthetase, synthetase, prostaglandin F2α, prostaglandin-D2 11-reductase, PGF synthetase, NADPH-dependent prostaglandin D2 11-keto reductase, and prostaglandin 11-keto reductase. This enzyme participates in arachidonic acid metabolism.
Structural studies
As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes 1RY0, 1RY8, 1VBJ, 1XF0, 1ZQ5, 2F38, and 2FGB.
References
- Reingold DF, Kawasaki A, Needleman P (1981). "A novel prostaglandin 11-keto reductase found in rabbit liver". Biochim. Biophys. Acta. 659 (1): 179–88. PMID 7248318. doi:10.1016/0005-2744(81)90282-5.
- Watanabe K, Shimizu T, Hayaishi O (1981). "Enzymatic conversion of prostaglandin-D2 to prostaglandin-F2α in the rat lung". Biochem. Int. 2: 603–610.
- Watanabe K, Yoshida R, Shimizu T, Hayaishi O (1985). "Enzymatic formation of prostaglandin F2α from prostaglandin H2 and D2. Purification and properties of prostaglandin F synthetase from bovine lung". J. Biol. Chem. 260 (11): 7035–41. PMID 3858278.
- Wong PY (1981). "Purification and partial characterization of prostaglandin D2 11-keto reductase in rabbit liver". Biochim. Biophys. Acta. 659 (1): 169–78. PMID 7248317. doi:10.1016/0005-2744(81)90281-3.
- Wong PY (1982). "Purification of PGD2 11-ketoreductase from rabbit liver". Methods Enzymol. 86: 117–25. PMID 7132748. doi:10.1016/0076-6879(82)86179-X.