Pierce Protein Assay
The Pierce Protein Assay is a method of protein quantification. It provides quick estimation of the protein amount in a given sample.[1]
Protocol
The assay is separated into three main parts:
- Preparation of the Diluted Albumin (BSA) Standards,
- Preparation of the bicinchoninic acid (BCA) working reagent,
- Quantification of proteins (using either test tube or microplate procedure)
Advantages and disadvantages
Advantages
- The method is able to detect as low as 25 µg/ml and up to 2000 µg/ml of protein in a 65 ul sample, using standard protocol.
- The method may be preferred for samples containing detergents or other reducing agents.
- The method has a fast detection speed and low protein-to-protein variability in comparison to the BCA or Coomassie (Bradford) Assays.
- The method has a stable end point.
Disadvantages
- The method has greater protein-to-protein variability than the BCA Assay.
References
- ↑ "Protein Assay Handbook" (PDF). Life technologies. Retrieved 9 April 2015.
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