Phikmvlikevirus
| Phikmvlikevirus | |
|---|---|
| Virus classification | |
| Group: | Group I (dsDNA) |
| Order: | Caudovirales |
| Family: | Podoviridae |
| Subfamily: | Autographivirinae |
| Genus: | Phikmvlikevirus |
| Type Species | |
| |
Phikmvlikevirus is a genus of viruses that infect bacteria. There are currently four species in this genus including the type species Pseudomonas phage phiKMV.[1][2] Bacteriophage phiKMV[3] and its relatives are known to be highly virulent phages, producing large (3–15 mm (0.12–0.59 in) diameter) clear plaques on a susceptible host.[4][5] The only reported exception is phage LKA1, which yields small plaques (1 mm (0.039 in)) surrounded by a halo. While all other P. aeruginosa-specific phikmvlikeviruses use the Type IV pili as primary receptor, LKA1 particles attach to the bacterial lipopolysaccharide layer.
Taxonomy
Group: dsDNA
- Family: Podoviridae
- Sub-Family: Autographivirinae
- Genus: Phikmvlikevirus
- Pantoea phage Limelight
- Pantoea phage Limezero
- Pseudomonas phage LKA1
- Pseudomonas phage phiKMV
Virology
Electron microscopic imaging of purified phage particles revealed these phages as typical members of the Podoviridae, with a head diameter of approximately 60 nm (2.4×10−6 in) and a stubby tail with a length of 8–10 nm. Although phiKMV phage resembles the well-studied podovirus T7 in overall genome architecture, it was the first known T7-like phage which encoded a single-subunit RNA polymerase gene downstream its DNA metabolism genes instead of in the early genomic region. Based on these properties, the genus Phikmvlikevirus is classified within the Autographivirinae.[6]
| Genus | Structure | Symmetry | Capsid | Genomic arrangement | Genomic segmentation |
|---|---|---|---|---|---|
| Phikmvlikevirus | Head-Tail | T=7 | Non-enveloped | Linear | Monopartite |
Life cycle
Viral replication is cytoplasmic. Entry into the host cell is achieved by adsorption into the host cell. DNA-templated transcription is the method of transcription. The virus exits the host cell by lysis, and holin/endolysin/spanin proteins. Bacteria serve as the natural host. Transmission routes are passive diffusion.[1]
| Genus | Host details | Tissue tropism | Entry details | Release details | Replication site | Assembly site | Transmission |
|---|---|---|---|---|---|---|---|
| Phikmvlikevirus | Bacteria | None | Injection | Lysis | Cytoplasm | Cytoplasm | Passive diffusion |
References
- 1 2 "Viral Zone". ExPASy. Retrieved 15 June 2015.
- 1 2 ICTV. "Virus Taxonomy: 2014 Release". Retrieved 15 June 2015.
- ↑ Lavigne, Rob; Burkal'tseva, Maria V.; Robben, Johan; Sykilinda, Nina N.; Kurochkina, Lidia P.; Grymonprez, Barbara; Jonckx, Bart; Krylov, Victor N.; et al. (2003). "The genome of bacteriophage φKMV, a T7-like virus infecting Pseudomonas aeruginosa". Virology. 312 (1): 49–59. PMID 12890620. doi:10.1016/S0042-6822(03)00123-5.
- ↑ Ceyssens, P.-J.; Lavigne, R.; Mattheus, W.; Chibeu, A.; Hertveldt, K.; Mast, J.; Robben, J.; Volckaert, G. (2006). "Genomic Analysis of Pseudomonas aeruginosa Phages LKD16 and LKA1: Establishment of the KMV Subgroup within the T7 Supergroup". Journal of Bacteriology. 188 (19): 6924–31. PMC 1595506
. PMID 16980495. doi:10.1128/JB.00831-06. - ↑ Lammens, E.; Ceyssens, P.-J.; Voet, M.; Hertveldt, K.; Lavigne, R.; Volckaert, G. (2009). "Representational Difference Analysis (RDA) of bacteriophage genomes". Journal of Microbiological Methods. 77 (2): 207–13. PMID 19232531. doi:10.1016/j.mimet.2009.02.006.
- ↑ Lavigne, Rob; Seto, Donald; Mahadevan, Padmanabhan; Ackermann, Hans-W.; Kropinski, Andrew M. (2008). "Unifying classical and molecular taxonomic classification: analysis of the Podoviridae using BLASTP-based tools". Research in Microbiology. 159 (5): 406–14. PMID 18555669. doi:10.1016/j.resmic.2008.03.005.