''para''-Nitrophenylphosphate

para-Nitrophenylphosphate

Names

IUPAC name

(4-nitrophenyl) dihydrogen phosphate

Other names

pNPP

Identifiers

CAS Number

330-13-2^N

3D model (JSmol)

Interactive image

ChEBI

CHEBI:17440^N

ChemSpider

369^N

ECHA InfoCard

100.005.777

PubChem CID

InChI

InChI=1S/C6H6NO6P/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12/h1-4H,(H2,10,11,12)^N
Key: XZKIHKMTEMTJQX-UHFFFAOYSA-N^N
InChI=1/C6H6NO6P/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12/h1-4H,(H2,10,11,12)
Key: XZKIHKMTEMTJQX-UHFFFAOYAU

SMILES

C1=CC(=CC=C1[N+](=O)[O-])OP(=O)(O)O

Properties

Chemical formula

C₆H₆NO₆P

Molar mass

317.14

Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

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Infobox references

para-Nitrophenylphosphate (pNPP) is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.^[1] Phosphatases catalyze the hydrolysis of pNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP). The resulting phenolate is yellow, with a maximal absorption at 405 nm.^[2] This property can be used to determine the activity of various phosphatases including alkaline phosphatase (AP) and protein tyrosine phosphatase (PTP). The substance is sensitive to light, and thus should be stored protected from light. This is also important after adding the substrate to the mixture and before reading. -20°C is the optimal storage temperature.

Refhttps://www.neb.com/products/p0757-p-nitrophenyl-phosphate-pnpperences

↑ Lorenz, Ulrike (2017-05-07). "Protein Tyrosine Phosphatase Assays". Current protocols in immunology / edited by John E. Coligan ... [et al.] CHAPTER: Unit–11.7. ISSN 1934-3671. PMC 3097125 . PMID 21462163. doi:10.1002/0471142735.im1107s93.
↑ MacKintosh, C. (1993). In D.G. Hardie (Ed.). Protein Phosphorylation: A Practical Approach. 221. New York: IRL Press.

External links

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