Monoraphidium neglectum

Monoraphidium
Scientific classification
Domain: Eukaryota
Kingdom: Viridiplantae
Phylum: Chlorophyta
Class: Chlorophyceae
Order: Sphaeropleales
Family: Selenastraceae
Genus: Monoraphidium
Species: M. neglectum
Binomial name
Monoraphidium neglectum
Heynig & Krienitz

Monoraphidium neglectum is a single-cell green alga of the family Selenastraceae.[1][2] There is interest in using M. neglectum for the production of biofuels because it can accumulate large quantities of triacylglycerides (TAGs).[3] In 2013, a draft nuclear, mitochondrial, and chloroplast genome was published with an estimated nuclear size of 68 Mb and approximately 16,761 genes.[4] The nuclear genome is diploid.[4] The mitochondrial genome of M. neglectum is 96 kb, which is twice as large as Nannochloropsis gaditana and nearly six times larger than the green alga Chlamydomonas reinhardtii, but contains fewer genes.[4][5] A nuclear transformation technique has been established for M. neglectum using electroporation as the method of DNA delivery.[6]

References

  1. ↑ Heynig, H.; Krienitz, L. (1982). "Monoraphidium neglectum n. sp. sowie einige Bemerkungen zu den Gattungen Monoraphidium, Chlorolobium und Keratococcus (Chlorococcales)". Archiv für Protistenkunde. 125: 335–344.
  2. ↑ See the NCBI webpage on Monoraphidium. Data extracted from the "NCBI taxonomy resources". National Center for Biotechnology Information. Retrieved 2017-03-17.
  3. ↑ Jaeger, D.; et al. (2016). "Label-free in vivo analysis of intracellular lipid droplets in the oleaginous microalga Monoraphidium neglectum by coherent Raman scattering microscopy". Scientific Report. 6: 35340. doi:10.1038/srep35340.
  4. 1 2 3 Bogen, C.; et al. (2013). "Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production". BMC Genomics. doi:10.1186/1471-2164-14-926.
  5. ↑ Radakovits, R.; et al. (2012). "Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana". Nature Communications. 3. doi:10.1038/ncomms1688.
  6. ↑ Jaeger, D.; et al. (2017). "Nuclear transformation and functional gene expression in the oleaginous microalga Monoraphidium neglectum". Journal of Biotechnology. doi:10.1016/j.jbiotec.2017.03.011.

Scientific references

Scientific databases


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