Langendorff heart

Native records of contractile activity of the left ventricle of isolated rat heart perfused under Langendorff technique. Curve A - contractile function of the heart is greatly depressed after ischemia-reperfusion. Curve B - a set of short ischemic episodes (ischemic preconditioning) before prolonged ischemia provides functional recovery of contractile activity of the heart at reperfusion.

The Langendorff heart or isolated perfused heart assay is a predominant in vitro technique used in pharmacological and physiological research using animals. It allows the examination of cardiac contractile strength and heart rate without the complications of an intact animal.[1]

Method

In the Langendorff preparation, the heart is removed from the animal's body, severing the blood vessels; it is then perfused in a reverse fashion via the aorta, usually with a nutrient rich, oxygenated solution (e.g. Krebs–Henseleit solution or Tyrode's solution). The backwards pressure causes the aortic valve to shut, forcing the solution into the coronary vessels, which normally supply the heart tissue with blood. This feeds nutrients and oxygen to the cardiac muscle, allowing it to continue beating for several hours after its removal from the animal. This is a useful preparation because it allows the addition of drugs (via the perfusate) and observation of their effect on the heart without the complications involved with in vivo experimentation, such as neuronal and hormonal effects from living animal.[2] This preparation also allows the organ to be digested into individual cells by adding collagenase to the perfusate. This can be done before the experiment as a technique for cell harvesting, or after the experiment to measure its effects at the cellular level.

See also

References

  1. ↑ Bell, R., Mocanu, M. & Yellon, D. Retrograde heart perfusion: The Langendorff technique of isolated heart perfusion. Journal of Molecular and Cellular Cardiology 50, 940-950 (2011).
  2. ↑ The Langendorff heart preparation—Reappraisal of its role as a research and teaching model for coronary vasoactive drugs, K.J. Broadley, Journal of Pharmacological Methods


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