LAPC4

LAPC4 cells are a cell line of human prostate cancer commonly used in the field of oncology.[1] The tissue was harvested from the lymph node metastasis of a male patient with hormone refractory prostate cancer.[2][3] This tissue was then xenografted into SCID mice and later harvested and plated on tissue culture dishes, where it can now be propagated as an immortalized prostate cancer cell line. The cell line adds to the limited number of prostate cancer cell models available to researchers.

Characteristics

LAPC4 are a lowly adherent, epithelial cell line with high Androgen receptor and Prostate specific antigen expression.[4] Unlike the other commonly utilized, Androgen receptor positive prostate cancer cell lines LNCaP and VCaP, LAPC4 have high expression of Keratin 5, a basal marker, as well as the luminal markers Keratin 8 and Keratin 18.[5]

The cells have an approximate doubling time of 72 hours under typical culture conditions.[6]

References

  1. ↑ "Growth of LAPC4 prostate cancer xenograft tumor is insensitive to 5α-reductase inhibitor dutasteride". Am J Clin Exp Urol. 2014. PMC 4219295 Freely accessible.
  2. ↑ Klein, KA; Reiter, RE; Redula, J; Moradi, H; Zhu, XL; Brothman, AR; Lamb, DJ; Marcelli, M; Belldegrun, A; Witte, ON; Sawyers, CL (April 1997). "Progression of metastatic human prostate cancer to androgen independence in immunodeficient SCID mice.". Nature medicine. 3 (4): 402–8. PMID 9095173.
  3. ↑ Russell, Pamela; Kingsley, Elizabeth. "Human Prostate Cancer Cell Lines". Methods in Molecular Medicine (PDF).
  4. ↑ van Bokhoven, A; Varella-Garcia, M; Korch, C; Johannes, WU; Smith, EE; Miller, HL; Nordeen, SK; Miller, GJ; Lucia, MS (1 November 2003). "Molecular characterization of human prostate carcinoma cell lines.". The Prostate. 57 (3): 205–25. PMID 14518029.
  5. ↑ van Bokhoven, A; Varella-Garcia, M; Korch, C; Johannes, WU; Smith, EE; Miller, HL; Nordeen, SK; Miller, GJ; Lucia, MS (1 November 2003). "Molecular characterization of human prostate carcinoma cell lines.". The Prostate. 57 (3): 205–25. PMID 14518029.
  6. ↑ "Cellosaurus cell line LAPC-4 (CVCL_4744)". web.expasy.org.


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