IZUMO1
Izumo sperm-egg fusion 1 is a protein that in humans is encoded by the IZUMO1 gene.[5]
Function
The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasma membrane binding and fusion.[6]
Studies have shown that male Izumo knockout mice are sterile because their sperm are unable to fuse to the oocyte membrane.[1] Izumo -/- mice produced morphologically normal sperm that were able to penetrate the zona pellucida, but could not fuse with to the eggs. In-vitro human experiments have also been conducted, suggesting that Izumo is required for human gamete fusion. [1]
Through the use of Western Blot analyses, it has been shown that Izumo is only expressed in the testis and is found on mature spermatozoa. [7] Izumo-1 located on mature spermatozoa that have undergone capacitation binds to its receptor Juno, which is located on the oolemma of eggs. [8]
References
- 1 2 3 GRCh38: Ensembl release 89: ENSG00000182264 - Ensembl, May 2017
- 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000064158 - Ensembl, May 2017
- ↑ "Human PubMed Reference:".
- ↑ "Mouse PubMed Reference:".
- ↑ "Entrez Gene: Izumo sperm-egg fusion 1".
- ↑ Inoue N, Ikawa M, Isotani A, Okabe M (2005). "The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs". Nature. 434 (7030): 234–8. PMID 15759005. doi:10.1038/nature03362.
- ↑ Ellerman DA, Pei J, Gupta S, Snell WJ, Myles D, Primakoff P. Izumo is part of a multiprotein family whose members form large complexes on mammalian sperm. Molecular reproduction and development. 2009;76(12):1188-1199.
- ↑ Bianchi E, Doe B, Goulding D, Sanger Mouse Genetics Project, Wright GJ. Juno is the egg Izumo receptor and is essential for mammalian fertilisation. Nature. 2014;508(7497):483-487.
Further reading
- Chu AY, Workalemahu T, Paynter NP, Rose LM, Giulianini F, Tanaka T, Ngwa JS, Qi Q, Curhan GC, Rimm EB, Hunter DJ, Pasquale LR, Ridker PM, Hu FB, Chasman DI, Qi L (2013). "Novel locus including FGF21 is associated with dietary macronutrient intake". Human Molecular Genetics. 22 (9): 1895–902. PMC 3612009
. PMID 23372041. doi:10.1093/hmg/ddt032. - Yatsenko AN, Roy A, Chen R, Ma L, Murthy LJ, Yan W, Lamb DJ, Matzuk MM (2006). "Non-invasive genetic diagnosis of male infertility using spermatozoal RNA: KLHL10 mutations in oligozoospermic patients impair homodimerization". Hum. Mol. Genet. 15 (23): 3411–9. PMID 17047026. doi:10.1093/hmg/ddl417.
- Granados-Gonzalez V, Aknin-Seifer I, Touraine RL, Chouteau J, Wolf JP, Levy R (2008). "Preliminary study on the role of the human IZUMO gene in oocyte-spermatozoa fusion failure". Fertil. Steril. 90 (4): 1246–8. PMID 18082733. doi:10.1016/j.fertnstert.2007.09.008.
- Ellerman DA, Pei J, Gupta S, Snell WJ, Myles D, Primakoff P (2009). "Izumo is part of a multiprotein family whose members form large complexes on mammalian sperm". Mol. Reprod. Dev. 76 (12): 1188–99. PMC 3779078 . PMID 19658160. doi:10.1002/mrd.21092.
- Grayson P, Civetta A (2012). "Positive Selection and the Evolution of izumo Genes in Mammals". Int J Evol Biol. 2012: 958164. PMC 3432370 . PMID 22957301. doi:10.1155/2012/958164.
- Inoue N, Ikawa M, Okabe M (2008). "Putative sperm fusion protein IZUMO and the role of N-glycosylation". Biochem. Biophys. Res. Commun. 377 (3): 910–4. PMID 18952059. doi:10.1016/j.bbrc.2008.10.073.
This article incorporates text from the United States National Library of Medicine, which is in the public domain.