Gonadotropin preparations

Gonadotropin preparations are drugs that mimic the physiological effects of gonadotropins, used therapeutically mainly as fertility medication for ovarian hyperstimulation and reversal of anovulation. For example, the so-called menotropins consist of LH and FSH extracted from human urine from menopausal women.^[1] There are also recombinant variants.

FSH and LH preparations

hMG (human Menopausal Gonadotrophins), FSH and LH prepared from human urine collected from postmenopausal women. First extracted in 1953.^[2] Injected intra-muscularily (IM) or subcutaneously (SC).

Generic

menotropins for injections, USP

Brands

• Menopur, 5 mL vials containing 75 IU FSH and 75 IU LH.
• Repronex, vials containing either 75 IU FSH and 75 IU LH, or 150 IU FSH and 150 IU LH.

The more common side effects of preparations containing FSH and LH are:^[3]

• Mild bloating
• Pain, swelling, or irritation injection site
• Rash at injection site or other part of body
• Stomach pain or pelvic pain

FSH preparations

Preparations of follicle-stimulating hormone (FSH) mainly include those derived from the urine of menopausal women, as well as recombinant preparations. The recombinant preparations are more pure and more easily administered, but they are more expensive. The urinary preparations are equally effective and less expensive, but are not as convenient to administer as they are available in vials versus injection pens. One study reported that users of the purified urinary FSH preparation Bravelle experienced less injection site pain compared to the recombinant preparation Follistim.^[4]

Urinary preparations

Purified urinary FSH (75 IU FSH and ≤ 2 IU of LH)

Generic

urofollitropin for injection, purified.

Brands

• Bravelle, U.S., (≤ 2 IU LH)
• Metrodin, U.S. and Canada, (≤ 1 IU LH)
• Fertinorm Hp, (Canada)

Highly purified urinary FSH (75 IU FSH and ≤ 0.1 IU LH/1000 IU FSH)

Generic

urofollitropin for injection, (highly) purified.

Dosage

typically 75 - 300, but as high as 600 IU / day

Brands

Fertinex (≤ 0.1 IU LH/1000 IU FSH)

Recombinant preparations

Follitropin alfa

Generic

Follitropin alfa injection

Brands

Gonal-f

Cinnal-f

Fertilex

Ovaleap

Bemfola

Follitropin beta

Generic

follitropin beta injection

Brands

Follistim

Puregon

Follitropin delta

Generic

follitropin delta injection

The package insert for Gonal-F states that based on physio-chemical tests and bioassays that follitropin beta and follitropin alfa are indistinguishable. Two studies showed no difference.^[5][6] However, a more recent study showed there may be a slight clinical difference, with the alfa form tending towards a higher pregnancy rate and the beta form tending towards a lower pregnancy rate, but with significantly higher estradiol (E2) levels.^[7]

The package insert for Puregon states that structural analysis shows that the amino acid sequence of follitropin beta is identical to that of natural human follicle stimulating hormone (hFSH). Further, the ogliosaccharide side chains are very similar, but not completely identical to that of natural hFSH. However, these small differences do not affect the bioactivity compared to natural hFSH.

Side effects of FSH preparations

Side effects of FSH preparations include:^[8]

• Local irritation at the injection site
• Feeling of fullness, bloating, and tenderness in the lower abdomen due to increasing size of the ovaries.
• Mood swings
• Fatigue

FSH analogues

Corifollitropin alfa

Merck received approval on February 15, 2010 from the European Commission for ELONVA (corifollitropin alfa) a long lasting single injection fusion protein lacking LH activity. Only one injection is required for the first seven days, replacing the first seven daily injections of conventional FSH. Initial results demonstrates similar pregnancy rates as daily recombinant FSH injections.^[9][10]

LH (Luteinizing hormone) preparations

Prepared from recombinant DNA.

Generic

lutropin alfa for injection

Brands

Luveris

hCG (human Chorionic Gonadotropin) preparations

Used to induce final maturation of follicle and subsequent ovulation. Also used for luteal phase support. Typically a single injection of 10,000 international units is used to induce ovulation. Recent data has shown that subcutaneous injection works as well as intramuscular injection, however this form of administration is not FDA approved.^[11]

Urinary preparations

Derived from the urine of pregnant women.

Generic

(human) chorionic gonadotropin for injection, USP

Brands

• Pregnyl (Merck/Schering-Plough)
• Follutein
• Profasi
• Novarel

Recombinant preparations

Generic

choriogonadotropin alfa for injection (recombinant human Chorionic Gonadotropin, r-hCG).

Brands

Ovidrel

References