Fermentative hydrogen production

Fermentative hydrogen production is the fermentative conversion of organic substrates to H2. Hydrogen produced in this manner is often called biohydrogen. The conversion is effected by bacteria and protozoa, which employ enzymes. Fermentative hydrogen production is one of several anaerobic conversions.

Dark vs photofermentation

Dark fermentation reactions do not require light energy. These are capable of constantly producing hydrogen from organic compounds throughout the day and night. Typically these reactions are coupled to the formation of carbon dioxide or formate. Important reactions that result in hydrogen production start with glucose, which is converted to acetic acid:[1]

C6H12O6 + 2 H2O → 2 CH3CO2H­ + 2 CO2 + 4 H2

A related reaction gives formate instead of carbon dioxide:

C6H12O6 + 2 H2O → 2 CH3CO2H­ + 2 HCO2H + 2 H2

These reactions are exegonic by 216 and 209 kcal/mol, respectively.

Using synthetic biology, bacteria can be genetically altered to enhance this reaction.[2][3]

Photofermentation differs from dark fermentation, because it only proceeds in the presence of light. Electrohydrogenesis is used in microbial fuel cells.

Bacteria strains

For example photo-fermentation with Rhodobacter sphaeroides SH2C can be employed to convert small molecular fatty acids into hydrogen.[4]

Enterobacter aerogenes is an outstanding hydrogen producer. It is an anaerobic facultative and mesophilic bacterium that is able to consume different sugars and in contrast to cultivation of strict anaerobes, no special operation is required to remove all oxygen from the fermenter. E. aerogenes has a short doubling time and high hydrogen productivity and evolution rate. Furthermore, hydrogen production by this bacterium is not inhibited at high hydrogen partial pressures; however, its yield is lower compared to strict anaerobes like Clostridia. A theoretical maximum of 4 mol H2/mol glucose can be produced by strict anaerobic bacteria. Facultative anaerobic bacteria such as E. aerogenes have a theoretical maximum yield of 2 mol H2/mol glucose.[5]

See also

References

  1. ↑ Thauer, R. K., "Biochemistry of Methanogenesis: a Tribute to Marjory Stephenson", Microbiology 1998, 144, 2377-2406. doi:10.1099/00221287-144-9-2377
  2. ↑ Synthetic biology and hydrogen
  3. ↑ Edwards, Chris (19 June 2008). "Synthetic biology aims to solve energy conundrum". The Guardian. London.
  4. ↑ High hydrogen yield from a two-step process of dark-and photo-fermentation of sucrose
  5. ↑ Asadi, Nooshin; Zilouei, Hamid (March 2017). "Optimization of organosolv pretreatment of rice straw for enhanced biohydrogen production using Enterobacter aerogenes". Bioresource Technology. 227: 335–344. doi:10.1016/j.biortech.2016.12.073.
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