Double-stranded uracil-DNA glycosylase

Double-stranded uracil-DNA glycosylase
Identifiers
EC number 3.2.2.28
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Double-stranded uracil-DNA glycosylase (EC 3.2.2.28, Mug, double-strand uracil-DNA glycosylase, Dug, dsUDG, double-stranded DNA specific UDG, dsDNA specific UDG, UdgB, G:T/U mismatch-specific DNA glycosylase, UDG) is an enzyme with systematic name uracil-double-stranded DNA deoxyribohydrolase (uracil-releasing).[1][2][3] This enzyme catalyses the following chemical reaction

Specifically hydrolyses mismatched double-stranded DNA and polynucleotides, releasing free uracil

This enzyme is not active on DNA containing a T/G mispair or single-stranded DNA.

References

  1. ↑ Barrett, T.E.; Scharer, O.D.; Savva, R.; Brown, T.; Jiricny, J.; Verdine, G.L.; Pearl, L.H. (1999). "Crystal structure of a thwarted mismatch glycosylase DNA repair complex". EMBO J. 18: 6599–6609. PMC 1171723 Freely accessible. PMID 10581234. doi:10.1093/emboj/18.23.6599.
  2. ↑ Sung, J.S.; Mosbaugh, D.W. (2000). "Escherichia coli double-strand uracil-DNA glycosylase: involvement in uracil-mediated DNA base excision repair and stimulation of activity by endonuclease IV". Biochemistry. 39: 10224–10235. PMID 10956012. doi:10.1021/bi0007066.
  3. ↑ Mokkapati, S.K.; Fernandez de Henestrosa, A.R.; Bhagwat, A.S. (2001). "Escherichia coli DNA glycosylase Mug: a growth-regulated enzyme required for mutation avoidance in stationary-phase cells". Mol. Microbiol. 41: 1101–1111. PMID 11555290. doi:10.1046/j.1365-2958.2001.02559.x.


This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.