Cobyrinate a,c-diamide synthase

Cobyrinate a,c-diamide synthase (EC 6.3.5.11, cobyrinic acid a,c-diamide synthetase, CbiA (gene)) is an enzyme.^[1] This enzyme catalyses the following chemical reaction

2 ATP + cobyrinate + 2 L-glutamine + 2 H₂O

\rightleftharpoons

2 ADP + 2 phosphate + cobyrinate a,c-diamide + 2 L-glutamate (overall reaction)

(1a) ATP + cobyrinate + L-glutamine + H₂O

\rightleftharpoons

ADP + phosphate + cobyrinate c-monamide + L-glutamate

(1b) ATP + cobyrinate c-monamide + L-glutamine + H₂O

\rightleftharpoons

ADP + phosphate + cobyrinate a,c-diamide + L-glutamate

This enzyme is the first glutamine amidotransferase that participates in the anaerobic biosynthetic pathway of adenosylcobalamin.

References

↑ Fresquet, V.; Williams, L.; Raushel, F.M. (2004). "Mechanism of cobyrinic acid a,c-diamide synthetase from Salmonella typhimurium LT2". Biochemistry. 43: 10619–10627. PMID 15311923. doi:10.1021/bi048972x.

Enzymes: CO CS and CN ligases (EC 6.1-6.3)
6.1: Carbon-Oxygen	Aminoacyl tRNA synthetase Alanine Arginine Asparagine Aspartate Cysteine D-alanine—poly(phosphoribitol) ligase Glutamate Glutamine Glycine Histidine Isoleucine Leucine Lysine Methionine Phenylalanine Proline Serine Threonine Tryptophan Tyrosine Valine
6.2: Carbon-Sulfur	Succinyl coenzyme A synthetase Acetyl—CoA synthetase Long-chain-fatty-acid—CoA ligase
6.3: Carbon-Nitrogen	Glutamine synthetase Ubiquitin ligase Cullin Von Hippel–Lindau tumor suppressor UBE3A Mdm2 Anaphase-promoting complex UBR1 Glutathione synthetase CTP synthetase Adenylosuccinate synthase Argininosuccinate synthase Holocarboxylase synthetase GMP synthase Asparagine synthetase Carbamoyl phosphate synthetase I II Glutamate–cysteine ligase

Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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