Cell (biology)

Cell

Onion (Allium) cells in different phases of the cell cycle, drawn by E. B. Wilson, 1900

A eukaryotic cell (left) and a prokaryotic cell (right)
Identifiers
TH H1.00.01.0.00001
FMA 68646

Anatomical terminology

Structure of an animal cell

The cell (from Latin cella, meaning "small room"[1]) is the basic structural, functional, and biological unit of all known living organisms. A cell is the smallest unit of life that can replicate independently, and cells are often called the "building blocks of life". The study of cells is called cell biology.

Cells consist of cytoplasm enclosed within a membrane, which contains many biomolecules such as proteins and nucleic acids.[2] Organisms can be classified as unicellular (consisting of a single cell; including bacteria) or multicellular (including plants and animals).[3] While the number of cells in plants and animals varies from species to species, humans contain more than 10 trillion (1012) cells.[4] Most plant and animal cells are visible only under a microscope, with dimensions between 1 and 100 micrometres.[5]

The cell was discovered by Robert Hooke in 1665, who named the biological units for their resemblance to cells inhabited by Christian monks in a monastery.[6][7] Cell theory, first developed in 1839 by Matthias Jakob Schleiden and Theodor Schwann, states that all organisms are composed of one or more cells, that cells are the fundamental unit of structure and function in all living organisms, that all cells come from preexisting cells, and that all cells contain the hereditary information necessary for regulating cell functions and for transmitting information to the next generation of cells.[8] Cells emerged on Earth at least 3.5 billion years ago.[9][10][11]

Anatomy

Comparison of features of prokaryotic and eukaryotic cells
Prokaryotes Eukaryotes
Typical organisms bacteria, archaea protists, fungi, plants, animals
Typical size ~ 1–5 µm[12] ~ 10–100 µm[12]
Type of nucleus nucleoid region; no true nucleus true nucleus with double membrane
DNA circular (usually) linear molecules (chromosomes) with histone proteins
RNA/protein synthesis coupled in the cytoplasm RNA synthesis in the nucleus
protein synthesis in the cytoplasm
Ribosomes 50S and 30S 60S and 40S
Cytoplasmic structure very few structures highly structured by endomembranes and a cytoskeleton
Cell movement flagella made of flagellin flagella and cilia containing microtubules; lamellipodia and filopodia containing actin
Mitochondria none one to several thousand
Chloroplasts none in algae and plants
Organization usually single cells single cells, colonies, higher multicellular organisms with specialized cells
Cell division binary fission (simple division) mitosis (fission or budding)
meiosis
Chromosomes single chromosome more than one chromosome
Membranes cell membrane Cell membrane and membrane-bound organelles

Cells are of two types, eukaryotic, which contain a nucleus, and prokaryotic, which do not. Prokaryotes are single-celled organisms, while eukaryotes can be either single-celled or multicellular.

Prokaryotic cells

Structure of a typical prokaryotic cell

Prokaryotic cells were the first form of life on Earth, characterised by having vital biological processes including cell signaling and being self-sustaining. They are simpler and smaller than eukaryotic cells, and lack membrane-bound organelles such as the nucleus. Prokaryotes include two of the domains of life, bacteria and archaea. The DNA of a prokaryotic cell consists of a single chromosome that is in direct contact with the cytoplasm. The nuclear region in the cytoplasm is called the nucleoid. Most prokaryotes are the smallest of all organisms ranging from 0.5 to 2.0 µm in diameter.[13]

A prokaryotic cell has three architectural regions:

Eukaryotic cells

Structure of a typical animal cell
Structure of a typical plant cell

Plants, animals, fungi, slime moulds, protozoa, and algae are all eukaryotic. These cells are about fifteen times wider than a typical prokaryote and can be as much as a thousand times greater in volume. The main distinguishing feature of eukaryotes as compared to prokaryotes is compartmentalization: the presence of membrane-bound organelles (compartments) in which specific metabolic activities take place. Most important among these is a cell nucleus,[3] an organelle that houses the cell's DNA. This nucleus gives the eukaryote its name, which means "true kernel (nucleus)". Other differences include:

Subcellular components

Illustration depicting major structures inside a eukaryotic animal cell[17]

All cells, whether prokaryotic or eukaryotic, have a membrane that envelops the cell, regulates what moves in and out (selectively permeable), and maintains the electric potential of the cell. Inside the membrane, the cytoplasm takes up most of the cell's volume. All cells (except red blood cells which lack a cell nucleus and most organelles to accommodate maximum space for hemoglobin) possess DNA, the hereditary material of genes, and RNA, containing the information necessary to build various proteins such as enzymes, the cell's primary machinery. There are also other kinds of biomolecules in cells. This article lists these primary components of the cell, then briefly describes their function.

Membrane

The cell membrane, or plasma membrane, is a biological membrane that surrounds the cytoplasm of a cell. In animals, the plasma membrane is the outer boundary of the cell, while in plants and prokaryotes it is usually covered by a cell wall. This membrane serves to separate and protect a cell from its surrounding environment and is made mostly from a double layer of phospholipids, which are amphiphilic (partly hydrophobic and partly hydrophilic). Hence, the layer is called a phospholipid bilayer, or sometimes a fluid mosaic membrane. Embedded within this membrane is a variety of protein molecules that act as channels and pumps that move different molecules into and out of the cell.[3] The membrane is said to be 'semi-permeable', in that it can either let a substance (molecule or ion) pass through freely, pass through to a limited extent or not pass through at all. Cell surface membranes also contain receptor proteins that allow cells to detect external signaling molecules such as hormones.

Cytoskeleton

A fluorescent image of an endothelial cell. Nuclei are stained blue, mitochondria are stained red, and microfilaments are stained green.

The cytoskeleton acts to organize and maintain the cell's shape; anchors organelles in place; helps during endocytosis, the uptake of external materials by a cell, and cytokinesis, the separation of daughter cells after cell division; and moves parts of the cell in processes of growth and mobility. The eukaryotic cytoskeleton is composed of microfilaments, intermediate filaments and microtubules. There are a great number of proteins associated with them, each controlling a cell's structure by directing, bundling, and aligning filaments.[3] The prokaryotic cytoskeleton is less well-studied but is involved in the maintenance of cell shape, polarity and cytokinesis.[18] The subunit protein of microfilaments is a small, monomeric protein called actin. The subunit of microtubules is a dimeric molecule called tubulin. Intermediate filaments are heteropolymers whose subunits vary among the cell types in different tissues. But some of the subunit protein of intermediate filaments include vimentin, desmin, lamin (lamins A, B and C), keratin (multiple acidic and basic keratins), neurofilament proteins (NF - L, NF - M).

Genetic material

Two different kinds of genetic material exist: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Cells use DNA for their long-term information storage. The biological information contained in an organism is encoded in its DNA sequence.[3] RNA is used for information transport (e.g., mRNA) and enzymatic functions (e.g., ribosomal RNA). Transfer RNA (tRNA) molecules are used to add amino acids during protein translation.

Prokaryotic genetic material is organized in a simple circular DNA molecule (the bacterial chromosome) in the nucleoid region of the cytoplasm. Eukaryotic genetic material is divided into different,[3] linear molecules called chromosomes inside a discrete nucleus, usually with additional genetic material in some organelles like mitochondria and chloroplasts (see endosymbiotic theory).

A human cell has genetic material contained in the cell nucleus (the nuclear genome) and in the mitochondria (the mitochondrial genome). In humans the nuclear genome is divided into 46 linear DNA molecules called chromosomes, including 22 homologous chromosome pairs and a pair of sex chromosomes. The mitochondrial genome is a circular DNA molecule distinct from the nuclear DNA. Although the mitochondrial DNA is very small compared to nuclear chromosomes,[3] it codes for 13 proteins involved in mitochondrial energy production and specific tRNAs.

Foreign genetic material (most commonly DNA) can also be artificially introduced into the cell by a process called transfection. This can be transient, if the DNA is not inserted into the cell's genome, or stable, if it is. Certain viruses also insert their genetic material into the genome.

Organelles

Organelles are parts of the cell which are adapted and/or specialized for carrying out one or more vital functions, analogous to the organs of the human body (such as the heart, lung, and kidney, with each organ performing a different function).[3] Both eukaryotic and prokaryotic cells have organelles, but prokaryotic organelles are generally simpler and are not membrane-bound.

There are several types of organelles in a cell. Some (such as the nucleus and golgi apparatus) are typically solitary, while others (such as mitochondria, chloroplasts, peroxisomes and lysosomes) can be numerous (hundreds to thousands). The cytosol is the gelatinous fluid that fills the cell and surrounds the organelles.

Eukaryotic

Human cancer cells with nuclei (specifically the DNA) stained blue. The central and rightmost cell are in interphase, so the entire nuclei are labeled. The cell on the left is going through mitosis and its DNA has condensed.
Diagram of an endomembrane system

Eukaryotic and prokaryotic

Structures outside the cell membrane

Many cells also have structures which exist wholly or partially outside the cell membrane. These structures are notable because they are not protected from the external environment by the semipermeable cell membrane. In order to assemble these structures, their components must be carried across the cell membrane by export processes.

Cell wall

Many types of prokaryotic and eukaryotic cells have a cell wall. The cell wall acts to protect the cell mechanically and chemically from its environment, and is an additional layer of protection to the cell membrane. Different types of cell have cell walls made up of different materials; plant cell walls are primarily made up of cellulose, fungi cell walls are made up of chitin and bacteria cell walls are made up of peptidoglycan.

Prokaryotic

Capsule

A gelatinous capsule is present in some bacteria outside the cell membrane and cell wall. The capsule may be polysaccharide as in pneumococci, meningococci or polypeptide as Bacillus anthracis or hyaluronic acid as in streptococci. Capsules are not marked by normal staining protocols and can be detected by India ink or methyl blue; which allows for higher contrast between the cells for observation.[20]:87

Flagella

Flagella are organelles for cellular mobility. The bacterial flagellum stretches from cytoplasm through the cell membrane(s) and extrudes through the cell wall. They are long and thick thread-like appendages, protein in nature. A different type of flagellum is found in archaea and a different type is found in eukaryotes.

Fimbria

A fimbria also known as a pilus is a short, thin, hair-like filament found on the surface of bacteria. Fimbriae, or pili are formed of a protein called pilin (antigenic) and are responsible for attachment of bacteria to specific receptors of human cell (cell adhesion). There are special types of specific pili involved in bacterial conjugation.

Cellular processes

Growth and metabolism

Between successive cell divisions, cells grow through the functioning of cellular metabolism. Cell metabolism is the process by which individual cells process nutrient molecules. Metabolism has two distinct divisions: catabolism, in which the cell breaks down complex molecules to produce energy and reducing power, and anabolism, in which the cell uses energy and reducing power to construct complex molecules and perform other biological functions. Complex sugars consumed by the organism can be broken down into simpler sugar molecules called monosaccharides such as glucose. Once inside the cell, glucose is broken down to make adenosine triphosphate (ATP),[3] a molecule that possesses readily available energy, through two different pathways.

Replication

Bacteria divide by binary fission, while eukaryotes divide by mitosis or meiosis.

Cell division involves a single cell (called a mother cell) dividing into two daughter cells. This leads to growth in multicellular organisms (the growth of tissue) and to procreation (vegetative reproduction) in unicellular organisms. Prokaryotic cells divide by binary fission, while eukaryotic cells usually undergo a process of nuclear division, called mitosis, followed by division of the cell, called cytokinesis. A diploid cell may also undergo meiosis to produce haploid cells, usually four. Haploid cells serve as gametes in multicellular organisms, fusing to form new diploid cells.

DNA replication, or the process of duplicating a cell's genome,[3] always happens when a cell divides through mitosis or binary fission. This occurs during the S phase of the cell cycle.

In meiosis, the DNA is replicated only once, while the cell divides twice. DNA replication only occurs before meiosis I. DNA replication does not occur when the cells divide the second time, in meiosis II.[21] Replication, like all cellular activities, requires specialized proteins for carrying out the job.[3]

Protein synthesis

An overview of protein synthesis.
Within the nucleus of the cell (light blue), genes (DNA, dark blue) are transcribed into RNA. This RNA is then subject to post-transcriptional modification and control, resulting in a mature mRNA (red) that is then transported out of the nucleus and into the cytoplasm (peach), where it undergoes translation into a protein. mRNA is translated by ribosomes (purple) that match the three-base codons of the mRNA to the three-base anti-codons of the appropriate tRNA. Newly synthesized proteins (black) are often further modified, such as by binding to an effector molecule (orange), to become fully active.

Cells are capable of synthesizing new proteins, which are essential for the modulation and maintenance of cellular activities. This process involves the formation of new protein molecules from amino acid building blocks based on information encoded in DNA/RNA. Protein synthesis generally consists of two major steps: transcription and translation.

Transcription is the process where genetic information in DNA is used to produce a complementary RNA strand. This RNA strand is then processed to give messenger RNA (mRNA), which is free to migrate through the cell. mRNA molecules bind to protein-RNA complexes called ribosomes located in the cytosol, where they are translated into polypeptide sequences. The ribosome mediates the formation of a polypeptide sequence based on the mRNA sequence. The mRNA sequence directly relates to the polypeptide sequence by binding to transfer RNA (tRNA) adapter molecules in binding pockets within the ribosome. The new polypeptide then folds into a functional three-dimensional protein molecule.

Motility

Unicellular organisms can move in order to find food or escape predators. Common mechanisms of motion include flagella and cilia.

In multicellular organisms, cells can move during processes such as wound healing, the immune response and cancer metastasis. For example, in wound healing in animals, white blood cells move to the wound site to kill the microorganisms that cause infection. Cell motility involves many receptors, crosslinking, bundling, binding, adhesion, motor and other proteins.[22] The process is divided into three steps – protrusion of the leading edge of the cell, adhesion of the leading edge and de-adhesion at the cell body and rear, and cytoskeletal contraction to pull the cell forward. Each step is driven by physical forces generated by unique segments of the cytoskeleton.[23][24]

Multicellularity

Cell specialization

Staining of a Caenorhabditis elegans which highlights the nuclei of its cells.

Multicellular organisms are organisms that consist of more than one cell, in contrast to single-celled organisms.[25]

In complex multicellular organisms, cells specialize into different cell types that are adapted to particular functions. In mammals, major cell types include skin cells, muscle cells, neurons, blood cells, fibroblasts, stem cells, and others. Cell types differ both in appearance and function, yet are genetically identical. Cells are able to be of the same genotype but of different cell type due to the differential expression of the genes they contain.

Most distinct cell types arise from a single totipotent cell, called a zygote, that differentiates into hundreds of different cell types during the course of development. Differentiation of cells is driven by different environmental cues (such as cell–cell interaction) and intrinsic differences (such as those caused by the uneven distribution of molecules during division).

Origin of multicellularity

Multicellularity has evolved independently at least 25 times,[26] including in some prokaryotes, like cyanobacteria, myxobacteria, actinomycetes, Magnetoglobus multicellularis or Methanosarcina. However, complex multicellular organisms evolved only in six eukaryotic groups: animals, fungi, brown algae, red algae, green algae, and plants.[27] It evolved repeatedly for plants (Chloroplastida), once or twice for animals, once for brown algae, and perhaps several times for fungi, slime molds, and red algae.[28] Multicellularity may have evolved from colonies of interdependent organisms, from cellularization, or from organisms in symbiotic relationships.

The first evidence of multicellularity is from cyanobacteria-like organisms that lived between 3 and 3.5 billion years ago.[26] Other early fossils of multicellular organisms include the contested Grypania spiralis and the fossils of the black shales of the Palaeoproterozoic Francevillian Group Fossil B Formation in Gabon.[29]

The evolution of multicellularity from unicellular ancestors has been replicated in the laboratory, in evolution experiments using predation as the selective pressure.[26]

Origins

The origin of cells has to do with the origin of life, which began the history of life on Earth.

Origin of the first cell

Stromatolites are left behind by cyanobacteria, also called blue-green algae. They are the oldest known fossils of life on Earth. This one-billion-year-old fossil is from Glacier National Park in the United States.

There are several theories about the origin of small molecules that led to life on the early Earth. They may have been carried to Earth on meteorites (see Murchison meteorite), created at deep-sea vents, or synthesized by lightning in a reducing atmosphere (see Miller–Urey experiment). There is little experimental data defining what the first self-replicating forms were. RNA is thought to be the earliest self-replicating molecule, as it is capable of both storing genetic information and catalyzing chemical reactions (see RNA world hypothesis), but some other entity with the potential to self-replicate could have preceded RNA, such as clay or peptide nucleic acid.[30]

Cells emerged at least 3.5 billion years ago.[9][10][11] The current belief is that these cells were heterotrophs. The early cell membranes were probably more simple and permeable than modern ones, with only a single fatty acid chain per lipid. Lipids are known to spontaneously form bilayered vesicles in water, and could have preceded RNA, but the first cell membranes could also have been produced by catalytic RNA, or even have required structural proteins before they could form.[31]

Origin of eukaryotic cells

The eukaryotic cell seems to have evolved from a symbiotic community of prokaryotic cells. DNA-bearing organelles like the mitochondria and the chloroplasts are descended from ancient symbiotic oxygen-breathing proteobacteria and cyanobacteria, respectively, which were endosymbiosed by an ancestral archaean prokaryote.

There is still considerable debate about whether organelles like the hydrogenosome predated the origin of mitochondria, or vice versa: see the hydrogen hypothesis for the origin of eukaryotic cells.

History of research

Hooke's drawing of cells in cork, 1665

See also

References

  1. 1 2 "Cell". Online Etymology Dictionary. Retrieved 31 December 2012.
  2. ↑ Cell Movements and the Shaping of the Vertebrate Body in Chapter 21 of Molecular Biology of the Cell fourth edition, edited by Bruce Alberts (2002) published by Garland Science.
    The Alberts text discusses how the "cellular building blocks" move to shape developing embryos. It is also common to describe small molecules such as amino acids as "molecular building blocks".
  3. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18  This article incorporates public domain material from the NCBI document "What Is a Cell?" (retrieved on 3 May 2013). 30 March 2004.
  4. ↑ Alberts, p. 2.
  5. ↑ Campbell, Neil A.; Brad Williamson; Robin J. Heyden (2006). Biology: Exploring Life. Boston, Massachusetts: Pearson Prentice Hall. ISBN 0-13-250882-6.
  6. ↑ Karp, Gerald (19 October 2009). Cell and Molecular Biology: Concepts and Experiments. John Wiley & Sons. p. 2. ISBN 9780470483374. Hooke called the pores cells because they re- minded him of the cells inhabited by monks living in a monastery.
  7. ↑ Alan Chong Tero (1990). Achiever's Biology. Allied Publishers. p. 36. ISBN 9788184243697. In 1665, an Englishman, Robert Hooke observed a thin slice of" cork under a simple microscope. (A simple microscope is a microscope with only one biconvex lens, rather like a magnifying glass). He saw many small box like structures. These reminded him of small rooms called "cells" in which Christian monks lived and meditated.
  8. ↑ Maton, Anthea (1997). Cells Building Blocks of Life. New Jersey: Prentice Hall. ISBN 0-13-423476-6.
  9. 1 2 Schopf, JW, Kudryavtsev, AB, Czaja, AD, and Tripathi, AB. (2007). Evidence of Archean life: Stromatolites and microfossils. Precambrian Research 158:141-155.
  10. 1 2 Schopf, JW (2006). Fossil evidence of Archaean life. Philos Trans R Soc Lond B Biol Sci 29;361(1470):869-85.
  11. 1 2 Peter Hamilton Raven; George Brooks Johnson (2002). Biology. McGraw-Hill Education. p. 68. ISBN 978-0-07-112261-0. Retrieved 7 July 2013.
  12. 1 2 Campbell Biology—Concepts and Connections. Pearson Education. 2009. p. 320.
  13. ↑ Microbiology : Principles and Explorations By Jacquelyn G. Black
  14. ↑ European Bioinformatics Institute, Karyn's Genomes: Borrelia burgdorferi, part of 2can on the EBI-EMBL database. Retrieved 5 August 2012
  15. ↑ Satir, Peter; Christensen, ST; Søren T. Christensen (2008-03-26). "Structure and function of mammalian cilia". Histochemistry and Cell Biology. Springer Berlin/Heidelberg. 129 (6): 687–693. PMC 2386530 Freely accessible. PMID 18365235. doi:10.1007/s00418-008-0416-9. 1432-119X. Retrieved 2009-09-12.
  16. ↑ PH Raven, Evert RF, Eichhorm SE (1999) Biology of Plants, 6th edition. WH Freeman, New York
  17. ↑ "Medical gallery of Blausen Medical 2014". WikiJournal of Medicine. 1 (2). doi:10.15347/wjm/2014.010.
  18. ↑ Michie K, Löwe J (2006). "Dynamic filaments of the bacterial cytoskeleton". Annu Rev Biochem. 75: 467–92. PMID 16756499. doi:10.1146/annurev.biochem.75.103004.142452.
  19. ↑ Ménétret JF, Schaletzky J, Clemons WM, et al. (December 2007). "Ribosome binding of a single copy of the SecY complex: implications for protein translocation". Mol. Cell. 28 (6): 1083–92. PMID 18158904. doi:10.1016/j.molcel.2007.10.034.
  20. ↑ Prokaryotes. Newnes. Apr 11, 1996. ISBN 9780080984735.
  21. ↑ Campbell Biology—Concepts and Connections. Pearson Education. 2009. p. 138.
  22. ↑ Revathi Ananthakrishnan; Allen Ehrlicher. "The Forces Behind Cell Movement". Biolsci.org. Retrieved 2009-04-17.
  23. ↑ Alberts B, Johnson A, Lewis J. et al. Molecular Biology of the Cell, 4e. Garland Science. 2002
  24. ↑ Ananthakrishnan, R; Ehrlicher, A (2007). "The Forces Behind Cell Movement". Int J Biol Sci. 3 (5): 303–317. PMC 1893118 Freely accessible. PMID 17589565. doi:10.7150/ijbs.3.303.
  25. ↑ Becker, Wayne M.; et al. (2009). The world of the cell. Pearson Benjamin Cummings. p. 480. ISBN 978-0-321-55418-5.
  26. 1 2 3 Grosberg RK, Strathmann RR. The evolution of multicellularity: A minor major transition? Annu Rev Ecol Evol Syst. 2007;38:621–654.
  27. ↑ http://public.wsu.edu/~lange-m/Documnets/Teaching2011/Popper2011.pdf
  28. ↑ Bonner, John Tyler (1998). "The Origins of Multicellularity" (PDF). Integrative Biology: Issues, News, and Reviews. 1 (1): 27–36. ISSN 1093-4391. doi:10.1002/(SICI)1520-6602(1998)1:1<27::AID-INBI4>3.0.CO;2-6. Archived from the original (PDF, 0.2 MB) on March 8, 2012. External link in |journal= (help)
  29. ↑ El Albani A, Bengtson S, Canfield DE, Bekker A, Macchiarelli R, Mazurier A, Hammarlund EU, Boulvais P, Dupuy JJ, Fontaine C, Fürsich FT, Gauthier-Lafaye F, Janvier P, Javaux E, Ossa FO, Pierson-Wickmann AC, Riboulleau A, Sardini P, Vachard D, Whitehouse M, Meunier A (1 July 2010). "Large colonial organisms with coordinated growth in oxygenated environments 2.1 Gyr ago". Nature. 466 (7302): 100–104. Bibcode:2010Natur.466..100A. ISSN 0028-0836. PMID 20596019. doi:10.1038/nature09166.
  30. ↑ Orgel LE (1998). "The origin of life--a review of facts and speculations". Trends Biochem Sci. 23 (12): 491–5. PMID 9868373. doi:10.1016/S0968-0004(98)01300-0.
  31. ↑ Griffiths G (December 2007). "Cell evolution and the problem of membrane topology". Nature Reviews. Molecular Cell Biology. 8 (12): 1018–24. PMID 17971839. doi:10.1038/nrm2287.
  32. ↑ "... I could exceedingly plainly perceive it to be all perforated and porous, much like a Honey-comb, but that the pores of it were not regular [..] these pores, or cells, [..] were indeed the first microscopical pores I ever saw, and perhaps, that were ever seen, for I had not met with any Writer or Person, that had made any mention of them before this. . ." – Hooke describing his observations on a thin slice of cork. Robert Hooke

Bibliography

  • Alberts, Bruce; Johnson, Alexander; Lewis, Julian; Morgan, David; Raff, Martin; Roberts, Keith; Walter, Peter (2015). Molecular Biology of the Cell (6th ed.). Garland Science. p. 2. ISBN 978-0815344322. 
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