Brain-heart infusion medium

Brain heart infusion media made from Difco powdered media

Brain-heart infusion medium or broth (BHI, BHIM, BHIB) is a highly nutritious general-purpose growth medium, often containing agar, for culturing fastidious and nonfastidious anaerobic microorganisms, such as streptococci, pneumococci and meningococci.[1] It is made by boiling cow[2] or porcine[3] hearts and brains. Boiling releases soluble factors into the broth. The broth can then be turned into powder for easy distribution. BHI broth contains sodium chloride which is used to differentiate enterococci from nonenterococcal group D streptococci. [4] BHI broth is often used in food safety, water safety, and antibiotic sensitivity tests.[3]

Brain Heart Infusion has proven to be effective in the cultivation of a wide variety of micro- organisms, including many types of pathogens. It has served as the base medium for new culture media formulations when supplemented with blood or with selective agents. Without supplementation, Brain Heart In fusion (BHI) Agar currently is recommended as a universal medium for aerobic bacteriology and for the primary recovery of fungi and Actinomycetales from clinical specimens and from nonclinical materials. BD Brain Heart Infusion (BHI) Agar derives it nutrients from the brain heart infusion, peptone and glucose components. The peptones and infusion are sources of organic nitrogen, carbon, sulfur, vitamins and trace substances. Glucose is the carbohydrate source which micro-organisms utilize by fermentative action. The medium is buffered through the use of disodium phosphate.

See also

References

  1. ↑ "Brain Heart Infusion Broth (Powder)". US Biological. Archived from the original on 28 March 2014.
  2. ↑ Bacterial nutrition Archived 2013-05-24 at the Wayback Machine.. Virtual Microbiology Textbook for Microbiology 102.
  3. 1 2 Acumedia Manufacturers. Brain-Heart Infusion Broth (7116). Neogen.
  4. ↑ BD. BHI (Brain Heart Infusion) Broth, 5 mL
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.