Bismarck brown Y

Bismarck brown Y
Names

Other names Bismarck brown Manchester brown Phenylene brown Basic Brown 1 C.I. 21000 Vesuvine BA
Identifiers
CAS Number	8005-77-4^N
3D model (JSmol)	Interactive image Interactive image
ChemSpider	13374^N
ECHA InfoCard	100.030.273
PubChem CID	13981
InChI InChI=1S/C18H18N8/c19-11-4-6-17(15(21)8-11)25-23-13-2-1-3-14(10-13)24-26-18-7-5-12(20)9-16(18)22/h1-10H,19-22H2/b25-23+,26-24+^N Key: BDFZFGDTHFGWRQ-OGGGYYITSA-N^N InChI=1/C18H18N8/c19-11-4-6-17(15(21)8-11)25-23-13-2-1-3-14(10-13)24-26-18-7-5-12(20)9-16(18)22/h1-10H,19-22H2/b25-23+,26-24+ Key: BDFZFGDTHFGWRQ-OGGGYYITBR
SMILES CC1=C(C=C(C=C1)N=NC2=C(C=C(C(=C2)C)N)N)N=NC3=C(C=C(C(=C3)C)N)N N(=N/c1ccc(cc1N)N)\c3cccc(/N=N/c2ccc(N)cc2N)c3
Properties
Chemical formula	C₁₈H₁₈N₈·2HCl
Molar mass	419.31 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
N verify (what is ^YN ?)
Infobox references

Bismarck brown Y is a diazo dye. It is used in histology for staining tissues. It stains acid mucins to yellow color. It can be used with live cells. It is also used to stain cartilage in bone specimens, as one of Kasten's Schiff-type reagents in the periodic acid-Schiff stain to stain cellulose, and in Feulgen stain to stain DNA.

It was more common in the past; today it is partially replaced by other stains.

Bismarck brown Y is a constituent of Papanicolaou stains.

It can also be used as a counterstain for Victoria blue R for staining of acid-fast microorganisms.

References

Lillie, RD (1977) Conn's Biological Stains, 9th ed. Baltimore: Williams & Wilkins, p. 145-146.

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