Acetylene hydratase

The W centre is bound to two molybdopterin cofactors.^[2] The mechanism is thought to involve attachment of acetylene to the metal followed by nucleophilic attack of water.^[3]

This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is acetaldehyde hydro-lyase (acetylene-forming). Other names in common use include AH, and acetaldehyde hydro-lyase. Acetylene hydratase participates in tetrachloroethene degradation.

References

↑ Rosner BM, Schink B (1995). "Purification and characterization of acetylene hydratase of Pelobacter acetylenicus, a tungsten iron-sulfur protein". J. Bacteriol. 177 (20): 5767–72. PMC 177396 . PMID 7592321.
↑ ten Brink, Felix (2014). "Chapter 2. Living on acetylene. A Primordial Energy Source". In Peter M.H. Kroneck and Martha E. Sosa Torres. The Metal-Driven Biogeochemistry of Gaseous Compounds in the Environment. Metal Ions in Life Sciences. 14. Springer. pp. 1–14. doi:10.1007/978-94-017-9269-1_2.
↑ Einsle O; Ullmann, GM; Messerschmidt, A; Schink, B; Kroneck, PM; Einsle, O (2007). "Structure of the non-redox-active tungsten/[4Fe:4S] enzyme acetylene hydratase". Proc. Natl. Acad. Sci. U.S.A. 104 (9): 3073–7. PMC 1805521 . PMID 17360611. doi:10.1073/pnas.0610407104.

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)
4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase
4.2.2: Acting on polysaccharides	Hyaluronate lyase
4.2.3: Acting on phosphates	Threonine synthase
4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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