Xiaoliang Sunney Xie

Xiaoliang Sunney Xie (Chinese: 谢晓亮; born 1962 in Beijing, China) is the Mallinckrodt Professor of Chemistry and Chemical Biology at Harvard University. Xie is considered a founding father of single-molecule enzymology.

Xie received a B.S. in chemistry from Peking University, followed by his Ph.D. in 1990 from the University of California at San Diego. He conducted postdoctoral research at the University of Chicago and in 1992 joined Pacific Northwest National Laboratory, where he later became a Chief Scientist. In 1999, he became the first full professor at Harvard University from the People's Republic of China since China's reform in 1978.

Among the first to conduct fluorescence studies of single molecules at room temperature in the early 1990s, his research group has contributed to the emergence of the field of single-molecule biophysical chemistry and its application to biology. His work focuses on single-molecule enzymology, protein conformational dynamics, and the study of gene expression and regulation in living cells. His group also pioneered CARS microscopy and stimulated Raman scattering microscopy, sensitive biomedical imaging techniques that allow 3D imaging of live cells and organisms based on vibrational spectroscopy.^[1] In addition, his group have led the developments of single-cell whole genome amplification for single cell genomics.

Honors and awards

• 2015: Albany Medical Center Prize ^[2]
• 2015: Peter Debye Award in Physical Chemistry, American Chemical Society ^[3]
• 2014: Fellow of the Optical Society of America
• 2013: NIH Director's Pioneer Award ^[4]
• 2013: Ellis R. Lippincott Award, Optical Society of America and Society for Applied Spectroscopy
• 2012: Harrison Howe Award, Rochester Section of the American Chemical Society
• 2012: Fellow of the American Academy of Microbiology
• 2012: Biophysical Society Founders Award
• 2011: Member of the National Academy of Sciences
• 2009: Ernest Orlando Lawrence Award
• 2008: Fellow of the American Physical Society
• 2008: Berthold Leibinger Zukunftspreis for Applied Laser Technology ^[5]
• 2008: Fellow of the American Academy of Arts and Sciences
• 2007: Willis E. Lamb Award for Laser Sciences and Quantum Optics ^[6]
• 2006: Fellow of Biophysical Society
• 2006: Fellow of the American Association for the Advancement of Science
• 2004: NIH Director's Pioneer Award ^[7]
• 2003: Raymond and Beverly Sackler Prize in the Physical Sciences
• 1996: Coblentz Award

Selected Literature

Single Cell Genomics

• Hou, Yu; Fan, Wei; Yan, Liying; Li, Rong; Lian, Ying; Huang, Jin; Li, Jinsen; Xu, Liya; Tang, Fuchou; Xie, X. Sunney; Qiao, Jie. "Genome Analyses of Single Human Oocytes," Cell 155, 1492-1506 DOI:10.1016/j.cell.2013.11.040 (2013)
• Ni, Xiaohui; Zhuo, Minglei; Su, Zhe; Duan, Jianchun; Gao, Yan; Wang, Zhijie; Zong, Chenghang; Bai, Hua; Chapman, Alec R.; Zhao, Jun; Xu, Liya; An, Tongtong; Ma, Qi; Wang, Yuyan; Wu, Meina; Sun, Yu; Wang, Shuhang; Li, Zhenxiang; Yang, Xiaodan; Yong, Jun; Su, Xiao-Dong; Lu, Youyong; Bai, Fan; Xie, X. Sunney; Wang, Jie. "Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients," PNAS 110, 21083-21088 DOI: 10.1073/pnas.1320659110 (2013)
• Zong, Chenghang; Lu, Sijia; Chapman, Alec R.; Xie, X. Sunney."Genome-Wide Detection of Single-Nucleotide and Copy-Number Variations of a Single Human Cell" Science 338, 1622-1626 (2012).
• Lu, Sijia; Zong, Chenghang; Fan, Wei; Yang, Mingyu; Li, Jinsen; Chapman, Alec R.; Zhu, Ping; Hu, Xuesong; Xu, Liya; Bai, Fan; Qiao, Jie; Tang, Fuchou; Li, Ruiqiang; Xie, X. Sunney. "Probing Meiotic Recombination and Aneuploidy of Single Sperm Cells by Whole-Genome Sequencing" Science 338, 1627-1630 (2012).
• Shiroguchi, Katsuyuki; Jia, Tony Z.; Sims, Peter A.; Xie, X. Sunney."Digital RNA Sequencing Minimizes Sequence-dependent Bias and Amplification Noise with Optimized Single-molecule Barcodes," PNAS 109, 1347-1352 (2012).
• Sims, Peter A.; Greenleaf, William J.; Duan, Haifeng; Xie, X. Sunney. "Fluorogenic DNA Sequencing in PDMS Microreactors," Nat Methods 8, 575-580 (2011).

Coherent Raman Microscopy

• Ji, Minbiao; Orringer, Daniel A.; Freudiger, Christian W.; Ramkissoon, Shakti; Liu, Xiaohui; Lau, Darryl; Golby, Alexandra J.; Norton, Isaiah; Hayashi, Marika; Agar, Nathalie Y. R.; Young, Geoffrey S.; Spino, Cathie; Santagata, Sandro; Camelo-Piragua, Sandra; Ligon, Keith L.; Sagher, Oren; Xie, X. Sunney. "Rapid, Label-Free Detection of Brain Tumors with Stimulated Raman Scattering Microscopy," Science Translational Medicine 5, 201ra119 DOI: 10.1126/scitranslmed.3005954 (2013).
• Wang, Meng C.; Min, Wei; Freudiger, Christian W.; Ruvkun, Gary; Xie, X. Sunney. "RNAi Screening for Fat Regulatory Genes with SRS Microscopy," Nat Methods 8, 135-138 (2011).
• Saar, Brian G.; Freudiger, Christian W.; Reichman, Jay; Stanley, C. Michael; Holtom, Gary R.; Xie, X. Sunney. "Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering," Science 330, 1368-1370 (2010).
• Freudiger, Christian W.; Min, Wei; Saar, Brian G.; Lu, Sijia; Holtom, Gary R.; He, Chengwei; Tsai, Jason C.; Kang, Jing X.; Xie, X. Sunney. "Label-Free Biomedical Imaging with High sensitivity by Stimulated Raman Scattering Microscopy," Science 322, 1857-1860 (2008).
• Evans, Conor L.; Potma, Eric O.; Puoris'haag, Mehron; Côté, Daniel; Lin, Charles P.; Xie, X. Sunney. "Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy" Proc Nat. Aca Sci 102, 16807-16812 (2005).
• Zumbusch, Andreas; Holtom, Gary R.; Xie, X. Sunney. "Vibrational Microscopy Using Coherent Anti-Stokes Raman Scattering," Phys Rev Lett 82, 4142-4145 (1999).

Gene Expression and Regulation - Live Cell Single Molecule Studies

• Taniguchi, Yuichi; Choi, Paul J.; Li, Gene-Wei; Chen, Huiyi; Babu, Mohan; Hearn, Jeremy; Emili, Andrew; Xie, X. Sunney. "Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells," Science 329, 533-538 (2010).
• Choi, Paul J.; Cai, Long; Frieda, Kirsten; Xie, X. Sunney. "A Stochastic Single-Molecule Event Triggers Phenotype Switching of a Bacterial Cell," Science 322, 442-446 (2008).
• Elf, Johan; Li, Gene-Wei; Xie, X. Sunney. "Probing Transcription Factor Dynamics at the Single-Molecule Level in a Living Cell," Science 316, 1191-1194 (2007).
• Yu, Ji; Xiao, Jie; Ren, Xiaojia; Lao, Kaiqin; Xie, X. Sunney. "Probing Gene Expression in Live Cells, One Protein Molecule at a Time," Science 311, 1600-1603 (2006).
• Cai, Long; Friedman, Nir; Xie, X. Sunney. "Stochastic Protein Expression in Individual Cells at the Single Molecule Level," Nature 440, 358-362 (2006).

Single Molecule Enzymology

• Chong, Shasha; Chen, Chongyi; Ge, Hao; Xie, X. Sunney. "Mechanism of Transcriptional Bursting in Bacteria," Cell 158, 314-326 DOI:10.1016/j.cell.2014.05.038 (2014)
• Kim, Sangjin; Broströmer, Erik; Xing, Dong; Jin, Jianshi; Chong, Shasha; Ge, Hao; Wang, Siyuan; Gu, Chan; Yang, Lijiang; Qin-gao, Yi; Su, Xiao-dong; Sun, Yujie; Xie, X. Sunney. "Probing Allostery Through DNA" Science 339, 816-819 (2013).
• Blainey, Paul C.; Luo, Guobin; Ko9u, S.C.; Mangel, Walter F.; Verdine, Gregory L.; Bagchi, Biman; Xie, X. Sunney. "Nonspecifically Bound Proteins Spin While Diffusing Along DNA," Nat Struct Mol Biol 16, 1224-1229 (2009).
• Luo, Guobin; Wang, Mina; Konigsberg, William H.; Xie, X. Sunney. "Single-Molecule and Ensemble Fluorescence Assays for a Functionally Important Conformational Change in T7 DNA Polymerase," Proc Natl Acad Sci USA 104, 12610-12615 (2007).
• English, Brian P.; Min, Wei; van Oijen, Antoine M.; Lee, Kang Taek; Luo, Guobin; Sun, Hongye; Cherayil, Binny J.; Kou, S.C.; Xie, X. Sunney. "Ever-Fluctuating Single Enzyme Molecules: Michaelis-Menten Equation Revisited," Nat Chem Bio 2, 87-94 (2006).
• Min, Wei; Luo, Guobin; Cherayil, Binny J.; Kou, S. C.; Xie, X. Sunney. "Observation of a Power-Law Memory Kernel for Fluctuations within a Single Protein Molecule," Phys Rev Lett 94, 198302(1-4) (2005).
• van Oijen, Atoine M. ; Blainey, Paul C. ; Crampton, Donald J. ; Richardson, Charles C. ; Ellenberger, Tom; Xie, X. Sunney. "Single-Molecule Kinetics of l Exonuclease Reveal Base Dependence and Dynamic Disorder," Science 301, 1235-1238 (2003).
• Yang, Haw; Luo, Guobin; Karnchanaphanurach, Pallop; Louie, Tai-Man; Rech, Ivan; Cova, Sergio; Xun, Luying; Xie, X. Sunney. "Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer," Science 302, 262-266 (2003).
• Lu, H. Peter; Xun, Luying; Xie, X. Sunney. "Single-Molecule Enzymatic Dynamics," Science 282, 1877-1882 (1998).

Single Molecule Spectroscopy and Imaging

• Chong, Shasha; Min, Wei; Xie, X. Sunney. "Ground-State Depletion Microscopy:Detection Sensitivity of Single-Molecule Optical Absorption at Room Temperature,"J Phys Chem Lett 1, 3316-3322 (2010).
• Min, Wei; Lu, Sijia; Chong, Shasha; Roy, Rahul; Holtom, Gary R.; Xie, X. Sunney. "Imaging Chromophores with Undetectable Fluorescence by Stimulated Emission Microscopy," Nature 461, 1105-1109 (2009).
• Sánchez, Erik J.; Novotny, Lukas; Xie, X. Sunney. "Near-field Fluorescence Microscopy Based on Two-photon Excitation with Metal Tips," Phys Rev Lett 82, 4014-4017 (1999).
• Lu, H. Peter; Xie, X. Sunney. "Single-Molecule Spectral Fluctuations at Room Temperature," Nature 385, 143-146 (1997).
• Sánchez, Erik J.; Novotny, Lukas; Holtom, G.R.; Xie, X. Sunney. "Room-Temperature Fluorescence Imaging and Spectroscopy of Single Molecules by Two-Photon Excitation," J Phys Chem A 101, 7019-7023 (1997).
• Xie, X. Sunney; Dunn, Robert C. "Probing Single Molecule Dynamics," Science 265, 361-364 (1994).

References

External links

• Homepage of the Xie Group at Harvard