U2AF2

U2 small nuclear RNA auxiliary factor 2

PDB rendering based on 1jmt.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
1JMT, 1O0P, 1OPI, 1U2F, 2G4B, 2HZC, 2M0G, 2U2F, 2YH0, 2YH1, 3VAF, 3VAG, 3VAH, 3VAI, 3VAJ, 3VAK, 3VAL, 3VAM, 4FXW, 4TU7, 4TU8, 4TU9

Identifiers

Symbols

U2AF2 ; U2AF65

External IDs

OMIM: 191318 HomoloGene: 110853 GeneCards: U2AF2 Gene

Gene ontology
Molecular function	• nucleotide binding • protein binding • enzyme binding • poly(A) RNA binding • C2H2 zinc finger domain binding
Cellular component	• Prp19 complex • nucleoplasm • spliceosomal complex • nuclear speck
Biological process	• mRNA splicing, via spliceosome • transcription from RNA polymerase II promoter • termination of RNA polymerase II transcription • mRNA processing • mRNA export from nucleus • RNA splicing • gene expression • mRNA 3'-end processing • positive regulation of RNA splicing • negative regulation of mRNA splicing, via spliceosome • regulation of mitophagy • positive regulation of protein targeting to mitochondrion
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 19:
55.65 – 55.67 Mb

Chr 7:
5.06 – 5.08 Mb

PubMed search

Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene.^[1]

Function

U2 auxiliary factor (U2AF), composed of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit, which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple alternatively spliced transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.^[2]

Interactions

U2AF2 has been shown to interact with:

PUF60,^[3]^[4]
SF1,^[3]^[5]^[6]
SFRS11,^[7]
SFRS2IP,^[8]
SRPK2,^[3]^[9]
U2 small nuclear RNA auxiliary factor 1^[3]^[10] and
WT1.^[11]

References

↑ Zamore PD, Patton JG, Green MR (Mar 1992). "Cloning and domain structure of the mammalian splicing factor U2AF". Nature 355 (6361): 609–14. doi:10.1038/355609a0. PMID 1538748.
↑ "Entrez Gene: U2AF2 U2 small nuclear RNA auxiliary factor 2".
1 2 3 4 Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
↑ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
↑ Berglund JA, Abovich N, Rosbash M (Mar 1998). "A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition". Genes Dev. 12 (6): 858–67. doi:10.1101/gad.12.6.858. PMC 316625. PMID 9512519.
↑ Abovich N, Rosbash M (May 1997). "Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals". Cell 89 (3): 403–12. doi:10.1016/s0092-8674(00)80221-4. PMID 9150140.
↑ Zhang WJ, Wu JY (Oct 1996). "Functional properties of p54, a novel SR protein active in constitutive and alternative splicing". Mol. Cell. Biol. 16 (10): 5400–8. PMC 231539. PMID 8816452.
↑ Zhang WJ, Wu JY (Feb 1998). "Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing". Mol. Cell. Biol. 18 (2): 676–84. PMC 108778. PMID 9447963.
↑ Wang HY, Lin W, Dyck JA, Yeakley JM, Songyang Z, Cantley LC, Fu XD (Feb 1998). "SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells". J. Cell Biol. 140 (4): 737–50. doi:10.1083/jcb.140.4.737. PMC 2141757. PMID 9472028.
↑ Zhang M, Zamore PD, Carmo-Fonseca M, Lamond AI, Green MR (Sep 1992). "Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit". Proc. Natl. Acad. Sci. U.S.A. 89 (18): 8769–73. doi:10.1073/pnas.89.18.8769. PMC 50002. PMID 1388271.
↑ Davies RC, Calvio C, Bratt E, Larsson SH, Lamond AI, Hastie ND (Oct 1998). "WT1 interacts with the splicing factor U2AF65 in an isoform-dependent manner and can be incorporated into spliceosomes". Genes Dev. 12 (20): 3217–25. doi:10.1101/gad.12.20.3217. PMC 317218. PMID 9784496.

Zhang M, Zamore PD, Carmo-Fonseca M, Lamond AI, Green MR (1992). "Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit". Proc. Natl. Acad. Sci. U.S.A. 89 (18): 8769–73. doi:10.1073/pnas.89.18.8769. PMC 50002. PMID 1388271.
Zamore PD, Green MR (1991). "Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution". EMBO J. 10 (1): 207–14. PMC 452631. PMID 1824937.
Zuo P, Maniatis T (1996). "The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing". Genes Dev. 10 (11): 1356–68. doi:10.1101/gad.10.11.1356. PMID 8647433.
Zhang WJ, Wu JY (1996). "Functional properties of p54, a novel SR protein active in constitutive and alternative splicing". Mol. Cell. Biol. 16 (10): 5400–8. PMC 231539. PMID 8816452.
Hong W, Bennett M, Xiao Y, Feld Kramer R, Wang C, Reed R (1997). "Association of U2 snRNP with the spliceosomal complex E". Nucleic Acids Res. 25 (2): 354–61. doi:10.1093/nar/25.2.354. PMC 146436. PMID 9016565.
Abovich N, Rosbash M (1997). "Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals". Cell 89 (3): 403–12. doi:10.1016/S0092-8674(00)80221-4. PMID 9150140.
Tronchère H, Wang J, Fu XD (1997). "A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA". Nature 388 (6640): 397–400. doi:10.1038/41137. PMID 9237760.
Fleckner J, Zhang M, Valcárcel J, Green MR (1997). "U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction". Genes Dev. 11 (14): 1864–72. doi:10.1101/gad.11.14.1864. PMID 9242493.
Zhang WJ, Wu JY (1998). "Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing". Mol. Cell. Biol. 18 (2): 676–84. PMC 108778. PMID 9447963.
Wang HY, Lin W, Dyck JA, Yeakley JM, Songyang Z, Cantley LC, Fu XD (1998). "SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells". J. Cell Biol. 140 (4): 737–50. doi:10.1083/jcb.140.4.737. PMC 2141757. PMID 9472028.
Berglund JA, Abovich N, Rosbash M (1998). "A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition". Genes Dev. 12 (6): 858–67. doi:10.1101/gad.12.6.858. PMC 316625. PMID 9512519.
Rudner DZ, Kanaar R, Breger KS, Rio DC (1998). "Interaction between subunits of heterodimeric splicing factor U2AF is essential in vivo". Mol. Cell. Biol. 18 (4): 1765–73. PMC 121406. PMID 9528748.
Lallena MJ, Martínez C, Valcárcel J, Correas I (1998). "Functional association of nuclear protein 4.1 with pre-mRNA splicing factors". J. Cell. Sci. 111 ( Pt 14) (14): 1963–71. PMID 9645944.
Gozani O, Potashkin J, Reed R (1998). "A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site". Mol. Cell. Biol. 18 (8): 4752–60. PMC 109061. PMID 9671485.
Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M (1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex". Nat. Genet. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529.
Davies RC, Calvio C, Bratt E, Larsson SH, Lamond AI, Hastie ND (1998). "WT1 interacts with the splicing factor U2AF65 in an isoform-dependent manner and can be incorporated into spliceosomes". Genes Dev. 12 (20): 3217–25. doi:10.1101/gad.12.20.3217. PMC 317218. PMID 9784496.
Ito T, Muto Y, Green MR, Yokoyama S (1999). "Solution structures of the first and second RNA-binding domains of human U2 small nuclear ribonucleoprotein particle auxiliary factor (U2AF(65))". EMBO J. 18 (16): 4523–34. doi:10.1093/emboj/18.16.4523. PMC 1171527. PMID 10449418.
Wang X, Bruderer S, Rafi Z, Xue J, Milburn PJ, Krämer A, Robinson PJ (1999). "Phosphorylation of splicing factor SF1 on Ser20 by cGMP-dependent protein kinase regulates spliceosome assembly". EMBO J. 18 (16): 4549–59. doi:10.1093/emboj/18.16.4549. PMC 1171529. PMID 10449420.
Ladomery MR, Slight J, Mc Ghee S, Hastie ND (1999). "Presence of WT1, the Wilm's tumor suppressor gene product, in nuclear poly(A)(+) ribonucleoprotein". J. Biol. Chem. 274 (51): 36520–6. doi:10.1074/jbc.274.51.36520. PMID 10593950.

PDB gallery

1jmt: X-ray Structure of a Core U2AF65/U2AF35 Heterodimer

1o0p: Solution Structure of the third RNA Recognition Motif (RRM) of U2AF65 in complex with an N-terminal SF1 peptide

1opi: SOLUTION STRUCTURE OF THE THIRD RNA RECOGNITION MOTIF (RRM) OF U2AF65 IN COMPLEX WITH AN N-TERMINAL SF1 PEPTIDE

1u2f: SOLUTION STRUCTURE OF THE FIRST RNA-BINDING DOMAIN OF HU2AF65

2hzc: Crystal structure of the N-terminal RRM of the U2AF large subunit

2u2f: SOLUTION STRUCTURE OF THE SECOND RNA-BINDING DOMAIN OF HU2AF65

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U2AF2

Function

Interactions

References

Further reading