TP53RK

TP53 regulating kinase
Identifiers
Symbols TP53RK ; BUD32; C20orf64; Nori-2; Nori-2p; PRPK; dJ101A2
External IDs OMIM: 608679 MGI: 1914050 HomoloGene: 6042 IUPHAR: 2248 GeneCards: TP53RK Gene
EC number 2.7.11.1
Orthologs
Species Human Mouse
Entrez 112858 76367
Ensembl ENSG00000172315 ENSMUSG00000042854
UniProt Q96S44 Q99PW4
RefSeq (mRNA) NM_033550 NM_023815
RefSeq (protein) NP_291028 NP_076304
Location (UCSC) Chr 20:
46.68 – 46.69 Mb		 Chr 2:
166.79 – 166.8 Mb
PubMed search

TP53-regulating kinase, also known as PRPK is an enzyme that in humans is encoded by the TP53RK gene.[1][2][3] This protein is a serine/threonine protein kinase that phosphorylates p53 at Ser15.

PRPK is part of the KEOPS/EKC complex, which participates in transcription control,[4] telomere regulation [5] and tRNA modification.[6]

Model organisms

Model organisms have been used in the study of TP53RK function. A conditional knockout mouse line called Trp53rktm1a(EUCOMM)Wtsi was generated at the Wellcome Trust Sanger Institute.[7] Male and female animals underwent a standardized phenotypic screen[8] to determine the effects of deletion.[9][10][11][12] Additional screens performed: - In-depth immunological phenotyping[13]

References

  1. ↑ Abe Y, Matsumoto S, Wei S, Nezu K, Miyoshi A, Kito K, Ueda N, Shigemoto K, Hitsumoto Y, Nikawa J, Enomoto Y (Nov 2001). "Cloning and characterization of a p53-related protein kinase expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes". The Journal of Biological Chemistry 276 (47): 44003–11. doi:10.1074/jbc.M105669200. PMID 11546806.
  2. ↑ Facchin S, Lopreiato R, Ruzzene M, Marin O, Sartori G, Götz C, Montenarh M, Carignani G, Pinna LA (Aug 2003). "Functional homology between yeast piD261/Bud32 and human PRPK: both phosphorylate p53 and PRPK partially complements piD261/Bud32 deficiency". FEBS Letters 549 (1-3): 63–6. doi:10.1016/S0014-5793(03)00770-1. PMID 12914926.
  3. ↑ "Entrez Gene: TP53RK TP53 regulating kinase".
  4. ↑ Kisseleva-Romanova E, Lopreiato R, Baudin-Baillieu A, Rousselle JC, Ilan L, Hofmann K, Namane A, Mann C, Libri D (Aug 2006). "Yeast homolog of a cancer-testis antigen defines a new transcription complex". The EMBO Journal 25 (15): 3576–85. doi:10.1038/sj.emboj.7601235. PMID 16874308.
  5. ↑ Downey M, Houlsworth R, Maringele L, Rollie A, Brehme M, Galicia S, Guillard S, Partington M, Zubko MK, Krogan NJ, Emili A, Greenblatt JF, Harrington L, Lydall D, Durocher D (Mar 2006). "A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator". Cell 124 (6): 1155–68. doi:10.1016/j.cell.2005.12.044. PMID 16564010.
  6. ↑ Srinivasan M, Mehta P, Yu Y, Prugar E, Koonin EV, Karzai AW, Sternglanz R (Mar 2011). "The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A". The EMBO Journal 30 (5): 873–81. doi:10.1038/emboj.2010.343. PMID 21183954.
  7. ↑ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
  8. 1 2 "International Mouse Phenotyping Consortium".
  9. ↑ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
  10. ↑ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
  11. ↑ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
  12. ↑ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
  13. 1 2 "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading

  • Bonaldo MF, Lennon G, Soares MB (Sep 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548. 
  • Miyoshi A, Kito K, Aramoto T, Abe Y, Kobayashi N, Ueda N (Apr 2003). "Identification of CGI-121, a novel PRPK (p53-related protein kinase)-binding protein". Biochemical and Biophysical Research Communications 303 (2): 399–405. doi:10.1016/S0006-291X(03)00333-4. PMID 12659830. 
  • Wang SC, Lien HC, Xia W, Chen IF, Lo HW, Wang Z, Ali-Seyed M, Lee DF, Bartholomeusz G, Ou-Yang F, Giri DK, Hung MC (Sep 2004). "Binding at and transactivation of the COX-2 promoter by nuclear tyrosine kinase receptor ErbB-2". Cancer Cell 6 (3): 251–61. doi:10.1016/j.ccr.2004.07.012. PMID 15380516. 
  • Abe Y, Takeuchi T, Imai Y, Murase R, Kamei Y, Fujibuchi T, Matsumoto S, Ueda N, Ogasawara M, Shigemoto K, Kito K (May 2006). "A Small Ras-like protein Ray/Rab1c modulates the p53-regulating activity of PRPK". Biochemical and Biophysical Research Communications 344 (1): 377–85. doi:10.1016/j.bbrc.2006.03.071. PMID 16600182. 


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