Synthetic rescue

Synthetic rescue (or synthetic recovery or synthetic viability[1] ) refers to a genetic interaction in which a cell that is nonviable or sensitive to a specific drug due to the presence of a genetic mutation becomes viable when the original mutation is combined with a second mutation in a different gene.[2] The second mutation can either be a loss-of-function mutation (equivalent to a knockout) or a gain-of-function mutation.[1][3]

Synthetic rescue could potentially be exploited for gene therapy, but it also provides information on the function of the genes involved in the interaction

Types of genetic suppression

Dosage-mediated suppression

Dosage-mediated suppression occurs when the suppression of the mutant phenotype is mediated by the over expression of a second suppressor gene. This can occur when the initial mutations destabilise a protein-protein interaction and over expression of the interacting protein bypass the negative effect of the initial mutation.

See also

Synthetic Lethality, Gene Therapy, and Complex Networks.

References

  1. 1 2 Puddu, F.; Oelschlaegel, T; Guerini, I; Geisler, NJ; Niu, H; Herzog, M; Salguero, I; Ochoa-Montaño, B; Viré, E; Sung, P; Adams, DJ; Keane, TM; Jackson, SP (2015). "Synthetic viability genomic screening defines Sae2 function in DNA repair". EMBO Journal 34 (11): 1509–1522. doi:10.15252/embj.201590973. PMID 25899817.
  2. ↑ Motter A. E., Gulbahce N., Almaas E. and Barabasi A.-L., Predicting synthetic rescues in metabolic networks, Molecular Systems Biology 4, 168 (2008).
  3. ↑ Nishikawa T., Gulbahce N., and Motter A. E., Spontaneous reaction silencing in metabolic optimization, PLoS Computational Biology 4, e1000236 (2008).
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