SORBS2

Sorbin and SH3 domain containing 2
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols SORBS2 ; ARGBP2; PRO0618
External IDs OMIM: 616349 HomoloGene: 83295 GeneCards: SORBS2 Gene
RNA expression pattern
More reference expression data
Orthologs
Species Human Mouse
Entrez 8470 234214
Ensembl ENSG00000154556 ENSMUSG00000031626
UniProt O94875 Q3UTJ2
RefSeq (mRNA) NM_001145670 NM_001205219
RefSeq (protein) NP_001139142 NP_001192148
Location (UCSC) Chr 4:
185.59 – 185.96 Mb		 Chr 8:
45.51 – 45.83 Mb
PubMed search

ArgBP2 protein, also referred to as Sorbin and SH3 domain-containing protein 2 is a protein that in humans is encoded by the SORBS2 gene.[1][2] ArgBP2 belongs to the a small family of adaptor proteins having sorbin homology (SOHO) domains. ArgBP2 is highly abundant in cardiac muscle cells at sarcomeric Z-disc structures, and is expressed in other cells at actin stress fibers and the nucleus.

Structure

ArgBP2 may exist in as many as 9 unique isoforms ranging from 52 kDa to 117 kDa (492 to 1100 amino acids).[2] ArgBP2 belongs to the a small family of adaptor proteins having sorbin homology (SOHO) domains and three SH3 domains, which regulate cell adhesion, cytoskeletal organization and growth factor signaling; other members include CAP/ponsin and vinexin.[3] The three SH3 domains are C-terminal, a serine-threonine rich domain[4] resides in the middle, and the sorbin homology (SoHo) domain is N-terminal. The SH3 domains interact with Arg/Abl, vinculin.[3] The SOHO domain interacts with flotillin found in lipid rafts.[5]

Function

The subcellular localization of this protein in epithelial and cardiac muscle cells suggests that ArgBP2 functions as an adapter protein to assemble signaling complexes in stress fibers, and that it is a potential link between Abl family kinases and the actin cytoskeleton. ArgBP2 contains several potential Abl phosphorylation sites;[4] Arg and c-Abl represent the mammalian members of the Abelson family of non-receptor protein-tyrosine kinases. In non-muscle cells, ArgBP2 bids Cbl which enhances the degradation of c-Abl;[6] and also Pyk2 which promotes cytoskeletal remodeling.[7] ArgBP2 binding with flotillin at lipid rafts may indicate a role for ArgBP2 in vesicle trafficking and signal transduction. Interestingly, flotillin in skeletal muscle cells exhibits a striated pattern[8] suggesting localization to T-tubules or sarcoplasmic reticular cisternae, though no precise role has been determined in cardiac cells. In cardiac muscle cells, pull-down experiments discovered ArgBP2 in complex with alpha actinin-2, vinculin, spectrin, paxillin, Pyk2 and flotillin, suggesting that ArgBP2 may be involved in myofibril assembly and Z-band signaling in cardiomyocytes,[9] though functional studies are necessary to elucidate specific mechanisms. ArgBP2 has been linked to hyertrophic signaling, as a potent paracrine-acting RNA molecule shown to induce cardiac hypertrophy in mice, miR-21, acts on both ArgBP2 and PDLIM5 to trigger the hypertrophic response.[10]

Clinical Significance

Elevated levels of serum ArgBP2 and coordinate decreases in ArgBP2 in myocardial tissue were detected in the very early phase from patients post-myocardial infarction who died within 7 hours of the insult.[11] Chromosome 4 pericentric inversion has been observed in 10 patients, with associated cardiac defects linked to terminal 4q35.1 deletions, which may affect SORBS2.[12]

Interactions

ArgBP2 has been shown to interact with:

References

  1. 1 2 3 Wang B, Golemis EA, Kruh GD (Jul 1997). "ArgBP2, a multiple Src homology 3 domain-containing, Arg/Abl-interacting protein, is phosphorylated in v-Abl-transformed cells and localized in stress fibers and cardiocyte Z-disks". The Journal of Biological Chemistry 272 (28): 17542–50. doi:10.1074/jbc.272.28.17542. PMID 9211900.
  2. 1 2 "Entrez Gene: SORBS2 sorbin and SH3 domain containing 2".
  3. 1 2 Kioka N, Ueda K, Amachi T (Feb 2002). "Vinexin, CAP/ponsin, ArgBP2: a novel adaptor protein family regulating cytoskeletal organization and signal transduction". Cell Structure and Function 27 (1): 1–7. doi:10.1247/csf.27.1. PMID 11937713.
  4. 1 2 Wang B, Golemis EA, Kruh GD (Jul 1997). "ArgBP2, a multiple Src homology 3 domain-containing, Arg/Abl-interacting protein, is phosphorylated in v-Abl-transformed cells and localized in stress fibers and cardiocyte Z-disks". The Journal of Biological Chemistry 272 (28): 17542–50. doi:10.1074/jbc.272.28.17542. PMID 9211900.
  5. ↑ Kimura A, Baumann CA, Chiang SH, Saltiel AR (Jul 2001). "The sorbin homology domain: a motif for the targeting of proteins to lipid rafts". Proceedings of the National Academy of Sciences of the United States of America 98 (16): 9098–103. doi:10.1073/pnas.151252898. PMC 55379. PMID 11481476.
  6. ↑ Soubeyran P, Barac A, Szymkiewicz I, Dikic I (Feb 2003). "Cbl-ArgBP2 complex mediates ubiquitination and degradation of c-Abl". The Biochemical Journal 370 (Pt 1): 29–34. doi:10.1042/BJ20021539. PMC 1223168. PMID 12475393.
  7. ↑ Haglund K, Ivankovic-Dikic I, Shimokawa N, Kruh GD, Dikic I (May 2004). "Recruitment of Pyk2 and Cbl to lipid rafts mediates signals important for actin reorganization in growing neurites". Journal of Cell Science 117 (Pt 12): 2557–68. doi:10.1242/jcs.01148. PMID 15128873.
  8. ↑ Voldstedlund M, Vinten J, Tranum-Jensen J (Nov 2001). "cav-p60 expression in rat muscle tissues. Distribution of caveolar proteins". Cell and Tissue Research 306 (2): 265–76. doi:10.1007/s004410100439. PMID 11702238.
  9. 1 2 3 Sanger JM, Wang J, Gleason LM, Chowrashi P, Dube DK, Mittal B, Zhukareva V, Sanger JW (Dec 2010). "Arg/Abl-binding protein, a Z-body and Z-band protein, binds sarcomeric, costameric, and signaling molecules". Cytoskeleton 67 (12): 808–23. doi:10.1002/cm.20490. PMC 3019100. PMID 20886612.
  10. ↑ Bang C, Batkai S, Dangwal S, Gupta SK, Foinquinos A, Holzmann A, Just A, Remke J, Zimmer K, Zeug A, Ponimaskin E, Schmiedl A, Yin X, Mayr M, Halder R, Fischer A, Engelhardt S, Wei Y, Schober A, Fiedler J, Thum T (May 2014). "Cardiac fibroblast-derived microRNA passenger strand-enriched exosomes mediate cardiomyocyte hypertrophy". The Journal of Clinical Investigation 124 (5): 2136–46. doi:10.1172/JCI70577. PMID 24743145.
  11. ↑ Kakimoto Y, Ito S, Abiru H, Kotani H, Ozeki M, Tamaki K, Tsuruyama T (2013). "Sorbin and SH3 domain-containing protein 2 is released from infarcted heart in the very early phase: proteomic analysis of cardiac tissues from patients". Journal of the American Heart Association 2 (6): e000565. doi:10.1161/JAHA.113.000565. PMID 24342996.
  12. ↑ Maurin ML, Labrune P, Brisset S, Le Lorc'h M, Pineau D, Castel C, Romana S, Tachdjian G (Feb 2009). "Molecular cytogenetic characterization of a 4p15.1-pter duplication and a 4q35.1-qter deletion in a recombinant of chromosome 4 pericentric inversion". American Journal of Medical Genetics. Part A 149A (2): 226–31. doi:10.1002/ajmg.a.32603. PMID 19161154.
  13. 1 2 Soubeyran P, Barac A, Szymkiewicz I, Dikic I (Feb 2003). "Cbl-ArgBP2 complex mediates ubiquitination and degradation of c-Abl". Biochem. J. 370 (Pt 1): 29–34. doi:10.1042/BJ20021539. PMC 1223168. PMID 12475393.
  14. ↑ Sanger JM, Wang J, Gleason LM, Chowrashi P, Dube DK, Mittal B, Zhukareva V, Sanger JW (Dec 2010). "Arg/Abl-binding protein, a Z-body and Z-band protein, binds sarcomeric, costameric, and signaling molecules". Cytoskeleton (Hoboken) 67 (12): 808–23. doi:10.1002/cm.20490. PMC 3019100. PMID 20886612.
  15. 1 2 Haglund K, Ivankovic-Dikic I, Shimokawa N, Kruh GD, Dikic I (May 2004). "Recruitment of Pyk2 and Cbl to lipid rafts mediates signals important for actin reorganization in growing neurites". J. Cell. Sci. 117 (Pt 12): 2557–68. doi:10.1242/jcs.01148. PMID 15128873.
  16. ↑ Kimura A, Baumann CA, Chiang SH, Saltiel AR (Jul 2001). "The sorbin homology domain: a motif for the targeting of proteins to lipid rafts". Proc. Natl. Acad. Sci. U.S.A. 98 (16): 9098–103. doi:10.1073/pnas.151252898. PMC 55379. PMID 11481476.
  17. ↑ Rönty M, Taivainen A, Moza M, Kruh GD, Ehler E, Carpen O (Oct 2005). "Involvement of palladin and alpha-actinin in targeting of the Abl/Arg kinase adaptor ArgBP2 to the actin cytoskeleton". Exp. Cell Res. 310 (1): 88–98. doi:10.1016/j.yexcr.2005.06.026. PMID 16125169.
  18. ↑ Kioka N, Ueda K, Amachi T (Feb 2002). "Vinexin, CAP/ponsin, ArgBP2: a novel adaptor protein family regulating cytoskeletal organization and signal transduction". Cell Struct. Funct. 27 (1): 1–7. doi:10.1247/csf.27.1. PMID 11937713.

Further reading

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