RIP-Chip

RIP-Chip is immunoprecipitation of an RNA-binding protein coupled to reverse transcription and a microarray. It has been used to find interactions between RNA and protein (one protein but many RNA species per analysis).[1][2][3]

An alternative methodology (RIP-Seq) is to sequence the RNAs that were pulled down using high-throughput sequencing rather than analyze them with a microarray.

A similar technique is ChIP-on-chip, which detects the binding of proteins to genomic DNA rather than RNA. A competing technique is CLIP-Seq, where the RNA binding protein is cross-linked to the RNA via the use of UV light, followed by nuclease digestion and analyzed with high-throughput sequencing.

References

  1. ↑ Townley-Tilson WH, Pendergrass SA, Marzluff WF, Whitfield ML (October 2006). "Genome-wide analysis of mRNAs bound to the histone stem–loop binding protein". RNA 12 (10): 1853–67. doi:10.1261/rna.76006. PMC 1581977. PMID 16931877.
  2. ↑ Khalil AM, Guttman M, Huarte M, et al. (July 2009). "Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression". Proc. Natl. Acad. Sci. U.S.A. 106 (28): 11667–72. doi:10.1073/pnas.0904715106. PMC 2704857. PMID 19571010.
  3. ↑ Hendrickson DG, Hogan DH, Herschlag D, Ferrell JEF, Brown PB (2008). Bähler, Jürg, ed. "Systematic Identification of mRNAs Recruited to Argonaute 2 by Specific microRNAs and Corresponding Changes in Transcript Abundance". PLoS ONE 3 (5): e2126. doi:10.1371/journal.pone.0002126. PMC 2330160. PMID 18461144.
This article is issued from Wikipedia - version of the Tuesday, December 22, 2015. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.