Quinolinate synthase

Quinolinate synthase (EC 2.5.1.72, NadA, QS, quinolinate synthetase) is an enzyme with system name glycerone phosphate:iminosuccinate alkyltransferase (cyclizing).^[1]^[2]^[3]^[4]^[5] This enzyme catalyses the following chemical reaction

glycerone phosphate + iminosuccinate

\rightleftharpoons

pyridine-2,3-dicarboxylate + 2 H₂O + phosphate

This iron-sulfur protein that requires a [4Fe-4S] cluster for activity.

References

↑ Ollagnier-de Choudens, S., Loiseau, L., Sanakis, Y., Barras, F. and Fontecave, M. (2005). "Quinolinate synthetase, an iron-sulfur enzyme in NAD biosynthesis". FEBS Lett. 579 (17): 3737–3743. doi:10.1016/j.febslet.2005.05.065. PMID 15967443.
↑ Katoh, A., Uenohara, K., Akita, M. and Hashimoto, T. (2006). "Early steps in the biosynthesis of NAD in Arabidopsis start with aspartate and occur in the plastid". Plant Physiol. 141 (3): 851–857. doi:10.1104/pp.106.081091. PMC 1489895. PMID 16698895.
↑ Sakuraba, H., Tsuge, H., Yoneda, K., Katunuma, N. and Ohshima, T. (2005). "Crystal structure of the NAD biosynthetic enzyme quinolinate synthase". J. Biol. Chem. 280 (29): 26645–26648. doi:10.1074/jbc.C500192200. PMID 15937336.
↑ Rousset, C., Fontecave, M. and Ollagnier de Choudens, S. (2008). "The [4Fe-4S] cluster of quinolinate synthase from Escherichia coli: Investigation of cluster ligands". FEBS Lett. 582 (19): 2937–2944. doi:10.1016/j.febslet.2008.07.032. PMID 18674537.
↑ Saunders, A.H. and Booker, S.J. (2008). "Regulation of the activity of Escherichia coli quinolinate synthase by reversible disulfide-bond formation". Biochemistry 47 (33): 8467–8469. doi:10.1021/bi801135y. PMC 3319134. PMID 18651751.

External links

Quinolinate synthase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferases: alkyl and aryl (EC 2.5)

2.5.1	Dimethylallyltranstransferase Thiaminase I Methionine adenosyltransferase Riboflavin synthase Dihydropteroate synthase Spermidine synthase Glutathione S-transferase Farnesyl-diphosphate farnesyltransferase Spermine synthase Alkylglycerone phosphate synthase Farnesyltransferase Geranylgeranyltransferase type 1 Porphobilinogen deaminase

Proteins: enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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