Protein O-GlcNAcase

Protein O-GlcNAcase
Identifiers
EC number 3.2.1.169
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Protein O-GlcNAcase (EC 3.2.1.169, OGA, glycoside hydrolase O-GlcNAcase, O-GlcNAcase, BtGH84, O-GlcNAc hydrolase) is an enzyme with system name (protein)-3-O-(N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

(1) [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O \rightleftharpoons [protein]-L-serine + N-acetyl-D-glucosamine
(2) [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O \rightleftharpoons [protein]-L-threonine + N-acetyl-D-glucosamine

References

  1. ↑ Gao, Y., Wells, L., Comer, F.I., Parker, G.J. and Hart, G.W. (2001). "Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic β-N-acetylglucosaminidase from human brain". J. Biol. Chem. 276: 9838–9845. doi:10.1074/jbc.M010420200. PMID 11148210.
  2. ↑ Wells, L., Gao, Y., Mahoney, J.A., Vosseller, K., Chen, C., Rosen, A. and Hart, G.W. (2002). "Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic β-N-acetylglucosaminidase, O-GlcNAcase". J. Biol. Chem. 277 (3): 1755–1761. doi:10.1074/jbc.M109656200. PMID 11788610.
  3. ↑ Cetinbas, N., Macauley, M.S., Stubbs, K.A., Drapala, R. and Vocadlo, D.J. (2006). "Identification of Asp174 and Asp175 as the key catalytic residues of human O-GlcNAcase by functional analysis of site-directed mutants". Biochemistry 45 (11): 3835–3844. doi:10.1021/bi052370b. PMID 16533067.
  4. ↑ Dennis, R.J., Taylor, E.J., Macauley, M.S., Stubbs, K.A., Turkenburg, J.P., Hart, S.J., Black, G.N., Vocadlo, D.J. and Davies, G.J. (2006). "Structure and mechanism of a bacterial β-glucosaminidase having O-GlcNAcase activity". Nat. Struct. Mol. Biol. 13 (4): 365–371. doi:10.1038/nsmb1079. PMID 16565725.
  5. ↑ Kim, E.J., Kang, D.O., Love, D.C. and Hanover, J.A. (2006). "Enzymatic characterization of O-GlcNAcase isoforms using a fluorogenic GlcNAc substrate". Carbohydr. Res. 341 (8): 971–982. doi:10.1016/j.carres.2006.03.004. PMID 16584714.
  6. ↑ Dong, D.L. and Hart, G.W. (1994). "Purification and characterization of an O-GlcNAc selective N-acetyl-β-D-glucosaminidase from rat spleen cytosol". J. Biol. Chem. 269 (30): 19321–19330. PMID 8034696.

External links

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