Plaque hybridization

Plaque hybridization is a technique used in Molecular biology for the identification of recombinant phages.[1] The procedure can also be used for the detection of differentially represented repetitive DNA. The technique involves the hybridization of isolated DNA from each of the thousands of the phage λ, from our cDNA to a label probe for our gene of study. This is followed by autoradiography to detect the position of the label.[2] The plaque hybridization procedure is similar to Colony hybridization but have some advantages over the later due to the smaller and well defined area of the filter to which the DNA bounds.[3]

References

  1. ↑ Dale, J. W.; Greenaway, P. J. (1985). "Nucleic Acids". Methods in molecular biology (Clifton, N.J.) 2: 285–8. doi:10.1385/0-89603-064-4:285. ISBN 0-89603-064-4. PMID 21374207. |chapter= ignored (help)
  2. ↑ Meister, G. A.; Lansman, R. A.; Grigliatti, T. A. (1995). "Simple plaque hybridization method for the detection of differentially represented repetitive DNA". BioTechniques 18 (2): 250–5. PMID 7727126.
  3. ↑ "Nylon Membranes for Colony and Plaque Hybridization". Life science. Retrieved June 4, 2015.
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