Peptide deformylase

In enzymology, a peptide deformylase (EC 3.5.1.88) is an enzyme that catalyzes the chemical reaction

formyl-L-methionyl peptide + H₂O formate + methionyl peptide

Thus, the two substrates of this enzyme are formyl-L-methionyl peptide and H₂O, whereas its two products are formate and methionyl peptide.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is formyl-L-methionyl peptide amidohydrolase.

Structural studies

As of late 2007, 34 structures have been solved for this class of enzymes, with PDB accession codes 1IX1, 1LM4, 1LM6, 1LME, 1LQW, 1LQY, 1LRU, 1LRY, 1N5N, 1Q1Y, 1S17, 1SV2, 1SZZ, 1V3Y, 1VEV, 1VEY, 1VEZ, 1WS0, 1WS1, 1XEM, 1XEN, 1XEO, 1Y6H, 1ZXZ, 1ZY0, 1ZY1, 2AI7, 2AI8, 2AI9, 2AIA, 2AIE, 2EW5, 2EW6, and 2EW7.

References

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• Chan MK, Gong W, Rajagopalan PT, Hao B, Tsai CM, Pei D (1997). "Crystal structure of the Escherichia coli peptide deformylase". Biochemistry. 36 (45): 13904–9. doi:10.1021/bi9711543. PMID 9374869. 
• Becker A, Schlichting I, Kabsch W, Schultz S, Wagner AF (1998). "Structure of peptide deformylase and identification of the substrate binding site". J. Biol. Chem. 273 (19): 11413–6. doi:10.1074/jbc.273.19.11413. PMID 9565550. 
• Becker A, Schlichting I, Kabsch W, Groche D, Schultz S, Wagner AF (1998). "Iron center, substrate recognition and mechanism of peptide deformylase". Nat. Struct. Biol. 5 (12): 1053–8. doi:10.1038/4162. PMID 9846875. 
• Rajagopalan PTR, Yu, XC and Pei D (1997). "Peptide deformylase: a new type of mononuclear iron protein". J. Am. Chem. Soc. 119 (50): 12418–12419. doi:10.1021/ja9734096. 
• Groche D, Becker A, Schlichting I, Kabsch W, Schultz S, Wagner AF (1998). "Isolation and crystallization of functionally competent Escherichia coli peptide deformylase forms containing either iron or nickel in the active site". Biochem. Biophys. Res. Commun. 246 (2): 342–6. doi:10.1006/bbrc.1998.8616. PMID 9610360. 
• Rajagopalan PT, Grimme S, Pei D (2000). "Characterization of cobalt(II)-substituted peptide deformylase: function of the metal ion and the catalytic residue Glu-133". Biochemistry. 39 (4): 779–90. doi:10.1021/bi9919899. PMID 10651644. 
• Hu YJ, Wei Y, Zhou Y, Rajagopalan PT, Pei D (1999). "Determination of substrate specificity for peptide deformylase through the screening of a combinatorial peptide library". Biochemistry. 38 (2): 643–50. doi:10.1021/bi9820412. PMID 9888804. 
• Ragusa S, Mouchet P, Lazennec C, Dive V, Meinnel T (1999). "Substrate recognition and selectivity of peptide deformylase Similarities and differences with metzincins and thermolysin". J. Mol. Biol. 289 (5): 1445–57. doi:10.1006/jmbi.1999.2832. PMID 10373378. 
• Giglione C, Pierre M, Meinnel T (2000). "Peptide deformylase as a target for new generation, broad spectrum antimicrobial agents". Mol. Microbiol. 36 (6): 1197–205. doi:10.1046/j.1365-2958.2000.01908.x. PMID 10931273. 
• Pei D (2001). "Peptide deformylase: a target for novel antibiotics?". Emerging Therapeutic Targets 5 (1): 23–40. doi:10.1517/14728222.5.1.23. PMID 15992166.