PNP agar

PNP Agar is an agar medium used in microbiology to identify Staphylococcus species that have phosphatase activity. The medium changes color when p-nitrophenylphosphate disodium (PNP) is dephosphorylated.^[1]

PNP agar is composed of Mueller-Hinton agar buffered to pH 5.6 to 5.8, with the addition of 0.495 mg/mL PNP.^[2]

References

↑ Janda, W.M.; K. Ristow; D. Novak (September 1994). "Evaluation of RapiDEC Staph for Identification of Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus saprophyticus". Journal of Clinical Microbiology 32 (9): 2056–2059. PMC 263941. PMID 7814525.
↑ Geary, C.; M. Stevens (October 1989). "Detection of phosphatase production by Staphylococcus species: a new method". Med Lab Sci 46 (4): 291–294. PMID 2559282.

Growth media / agar plates

Selective media

Gram positive

Actinobacteria	Mycobacterium tuberculosis Löwenstein–Jensen medium Middlebrook 7H9 Broth Middlebrook 7H10 Agar Middlebrook 7H11 Agar Mycoplasma pneumoniae Eaton's agar

Firmicutes	Corynebacterium diphtheriae Hoyle's agar Enterococcus Bile esculin agar Lactobacillus MRS agar Lactococcus M17 agar Staphylococcus Mannitol salt agar

Gram negative

Alphaproteobacteria	Brucella Brucella agar

Betaproteobacteria	Neisseria Thayer-Martin agar

Gammaproteobacteria	Bordetella Bordet-Gengou agar Enterobacteriaceae VRBD agar Haemophilus influenzae/Legionella pneumophila Buffered charcoal yeast extract agar Pseudomonas aeruginosa Cetrimide agar Salmonella XLT agar DCA agar Salmonella/Shigella XLD agar

Differential media

Fungal media

Nonselective media

Other/ungrouped media

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