Nucleic acid methods
For protein methods, see Protein methods.
Nucleic acid methods are the techniques used to study nucleic acids (DNA and RNA).
Purification
Quantification
- Abundance in weight: spectroscopic quantification
- Absolute abundance in number: quantitative PCR
- High-throughput relative abundance: DNA microarray
- High-throughput absolute abundance: SAGE
- Size: gel electrophoresis
Synthesis
- De novo: oligonucleotide synthesis
- Amplification: PCR
Kinetics
Other
- Bisulfite sequencing
- DNA sequencing
- Expression cloning
- Fluorescence in situ hybridization
- Lab-on-a-chip
- Nucleic acid simulations
- Northern blot
- Nuclear run-on assay
- Radioactivity in biological research
- Southern blot
- Sucrose gradient centrifugation
- Several bioinformatics methods, such as RNA structure prediction
See also
References
- ↑ Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics 21 (4): 618–626. doi:10.1016/j.bios.2004.12.024. PMID 16202875.
- ↑ Dixon, Matthew C. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques 19 (3): 151–158. PMC 2563918. PMID 19137101.
External links
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