Cnidocyte

"Nematocyst" redirects here. For the subcellular structure in dinoflagellates, see nematocyst (dinoflagellate). For the floating structure formed by algae, see pneumatocyst.
Nomarski micrograph of a ruthenium red-stained nematocyst from Aiptasia pallida, the pale anemone. The red dye stains the polyanionic venom proteins found inside the capsule of this partially discharged nematocyst.

A cnidocyte (also known as a cnidoblast or nematocyte) is an explosive cell containing one giant secretory organelle or cnida (plural cnidae) that defines the phylum Cnidaria (corals, sea anemones, hydrae, jellyfish, etc.). Cnidae are used for prey capture and defense from predators. Despite being morphologically simple, lacking a skeleton and many species being sessile, cnidarians prey on fish and crustaceans. A cnidocyte fires a structure that contains the toxin, from a characteristic subcellular organelle called a cnidocyst (also known as a cnida or nematocyst). This is responsible for the stings delivered by a cnidarian.

Structure and function

A discharged nematocyst seen under a scanning electron microscope

Each cnidocyte contains an organelle called a cnida or cnidocyst (e.g. nematocyst, ptychocyst or spirocyst), which comprises a bulb-shape capsule containing a coiled hollow tubule structure attached to it. The immature cnidocyte is referred to as a cnidoblast. The externally oriented side of the cell also has a hair-like trigger called a cnidocil. When the trigger is activated, the tubule shaft of the cnidocyst is ejected and in the case of the penetrant nematocyst, the forcefully ejected tubule penetrates the target organism. This discharge takes no more than a few microseconds, and is able to reach accelerations of about 40,000 g.[1][2] Recent research suggests the process to occur as fast as 700 nanoseconds, thus reaching an acceleration of up to 5,410,000 g.[3] After penetration, the toxic content of the nematocyst is injected into the target organism, allowing the sessile cnidarian to devour it.

Discharge mechanism

A diagram of the discharge mechanism of a nematocyst

Cnidae capsule stores a large concentration of calcium ions, which are released from the capsule into the cytoplasm of the cnidocyte when the trigger is activated. This causes a large concentration gradient of calcium across the cnidocyte plasma membrane. The resulting osmotic pressure causes a rapid influx of water into the cell. This increase in water volume in the cytoplasm forces the coiled cnidae tubule to eject rapidly. Prior to discharge the coiled cnidae tubule exists inside the cell in an "inside out" condition. The back pressure resulting from the influx of water into the cnidocyte together with the opening of the capsule tip structure or operculum, triggers the forceful eversion of the cnidae tubule causing it to right itself as it comes rushing out of the cell with enough force to impale a prey organism.

Prey detection

Since cnidae are "single use" cells, and this costs a lot of energy, in order to regulate discharge, cnidocytes are connected as "batteries", containing several types of cnidocytes connected to supporting cells and neurons. The supporting cells contain chemosensors, which, together with the mechanoreceptor on the cnidocyte (cnidocil), allow only the right combination of stimuli to cause discharge, such as prey swimming, and chemicals found in prey cuticle or cuteous tissue. This prevents the cnidarian from stinging itself although sloughed off cnidae can be induced to fire independently.

Types of cnidae

Over 30 types of cnidae are found in different cnidarians. They can be divided into the following groups:

  1. Penetrant: a harpoon-like structure used to penetrate, referred to as nematocysts
  2. Glutinant: a sticky surface used to stick to prey, referred to as ptychocysts and found on burrowing (tube) anemones, which help create the tube in which the animal lives
  3. Volvent: a lasso-like string that is fired at prey and wraps around a cellular projection on the prey, referred to as spirocysts

Depending on the species, one or several types can appear simultaneously on the organism. The specific representation of cnidae is referred to as the cnidome of that species and may represent a dynamic aspect of the cnidarian species that is responsive to prey availability or the developmental stage of the organism.

Nematocyst toxicity

Nematocysts from Chironex fleckeri (400x magnification)

Nematocysts are very efficient weapons. A single nematocyst has been shown to suffice in paralyzing a small arthropod (Drosophila larva). The most deadly cnidocytes (to humans, at least) are found on the body of a box jellyfish.[4][5][6] One member of this family, the sea wasp, Chironex fleckeri, is "claimed to be the most venomous marine animal known," according to the Australian Institute of Marine Science. It can cause excruciating pain to humans, sometimes followed by death. Other cnidarians, such as the jellyfish Cyanea capillata (the "Lion's Mane" made famous by Sherlock Holmes) or the hydrozoan Physalia physalis (Portuguese man o' war, "Bluebottle") can cause extremely painful and sometimes fatal stings. On the other hand, aggregating sea anemones may have the lowest sting intensity, perhaps due to the inability of the nematocysts to penetrate the skin, creating a feeling similar to touching sticky candies. Besides feeding and defense, sea anemone and coral colonies use cnidocytes to sting one another in order to defend or win space.[7]

Venom from animals such as cnidarians, scorpions and spiders may be species-specific. A substance that is weakly toxic for humans or other mammals may be strongly toxic to the natural prey or predators of the venomous animal. Such specificity has been used to create new medicines and bioinsecticides.

Animals in the phylum Ctenophora ("sea-gooseberries" or "comb jellies") are transparent and jelly-like but have no nematocysts, and are harmless to humans.

Certain types of sea slugs, such as the nudibranch aeolids, are known to undergo kleptocnidae (in addition to kleptoplasty), whereby the organisms store nematocysts of digested prey at the tips of their cerata.

See also

References

  1. ↑ Holstein T., Tardent P. (1984). "An ultrahigh-speed analysis of exocytosis: nematocyst discharge". Science 223 (4638): 830–833. doi:10.1126/science.6695186. PMID 6695186.
  2. ↑ Kass-Simon G., Scappaticci A. A. Jr (2002). "The behavioral and developmental physiology of nematocysts" (PDF). Canadian Journal of Zoology 80: 1772–1794. doi:10.1139/Z02-135. Retrieved 2012-10-25.
  3. ↑ Nüchter Timm, Benoit Martin, Engel Ulrike, Özbek Suat, Holstein Thomas W. (2006). "Nanosecond-scale kinetics of nematocyst discharge". Current Biology 16 (9): R316–R318. doi:10.1016/j.cub.2006.03.089. Retrieved 2012-10-25.
  4. ↑ Tibballs J (December 2006). "Australian venomous jellyfish, envenomation syndromes, toxins and therapy". Toxicon 48 (7): 830–59. doi:10.1016/j.toxicon.2006.07.020. PMID 16928389.
  5. ↑ Brinkman D, Burnell J (November 2007). "Identification, cloning and sequencing of two major venom proteins from the box jellyfish, Chironex fleckeri". Toxicon 50 (6): 850–60. doi:10.1016/j.toxicon.2007.06.016. PMID 17688901.
  6. ↑ Brinkman D, Burnell J (April 2008). "Partial purification of cytolytic venom proteins from the box jellyfish, Chironex fleckeri". Toxicon 51 (5): 853–63. doi:10.1016/j.toxicon.2007.12.017. PMID 18243272.
  7. ↑ http://www.youtube.com/watch?v=WQEiYWGitKs

External links

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