NDC80

NDC80 kinetochore complex component

PDB rendering based on 2igp.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
2IGP, 2VE7, 3IZ0

Identifiers

Symbols

NDC80 ; HEC; HEC1; HsHec1; KNTC2; TID3; hsNDC80

External IDs

OMIM: 607272 MGI: 1914302 HomoloGene: 38141 ChEMBL: 5660 GeneCards: NDC80 Gene

Gene ontology
Molecular function	• structural constituent of cytoskeleton • protein binding • identical protein binding
Cellular component	• chromosome, centromeric region • kinetochore • condensed chromosome kinetochore • condensed nuclear chromosome kinetochore • condensed nuclear chromosome outer kinetochore • nucleus • cytosol • membrane • Ndc80 complex
Biological process	• mitotic sister chromatid segregation • establishment of mitotic spindle orientation • mitotic cell cycle • mitotic spindle organization • chromosome segregation • mitotic nuclear division • small GTPase mediated signal transduction • attachment of spindle microtubules to kinetochore • cell division • attachment of mitotic spindle microtubules to kinetochore
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 18:
2.57 – 2.62 Mb

Chr 17:
71.5 – 71.53 Mb

PubMed search

Kinetochore protein NDC80 homolog is a protein that in humans is encoded by the NDC80 gene.^[1]^[2]^[3]

Function

HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]^[3]

Interactions

NDC80 has been shown to interact with MIS12,^[4]^[5] NEK2^[6]^[7] and PSMC2.^[7]

References

↑ Chen Y, Riley DJ, Chen PL, Lee WH (Oct 1997). "HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis". Molecular and Cellular Biology 17 (10): 6049–56. doi:10.1128/mcb.17.10.6049. PMC 232454. PMID 9315664.
↑ Martin-Lluesma S, Stucke VM, Nigg EA (Sep 2002). "Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2". Science 297 (5590): 2267–70. doi:10.1126/science.1075596. PMID 12351790.
1 2 "Entrez Gene: NDC80 NDC80 homolog, kinetochore complex component (S. cerevisiae)".
↑ Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR, Oegema K, Desai A (Sep 2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension". Genes & Development 18 (18): 2255–68. doi:10.1101/gad.1234104. PMC 517519. PMID 15371340.
↑ Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell Biology 6 (11): 1135–41. doi:10.1038/ncb1187. PMID 15502821.
↑ Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH (Dec 2002). "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". The Journal of Biological Chemistry 277 (51): 49408–16. doi:10.1074/jbc.M207069200. PMID 12386167.
1 2 Chen Y, Sharp ZD, Lee WH (Sep 1997). "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins". The Journal of Biological Chemistry 272 (38): 24081–7. doi:10.1074/jbc.272.38.24081. PMID 9295362.

Chen Y, Sharp ZD, Lee WH (Sep 1997). "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins". The Journal of Biological Chemistry 272 (38): 24081–7. doi:10.1074/jbc.272.38.24081. PMID 9295362.
Zheng L, Chen Y, Lee WH (Aug 1999). "Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins". Molecular and Cellular Biology 19 (8): 5417–28. doi:10.1128/mcb.19.8.5417. PMC 84384. PMID 10409732.
Zheng L, Chen Y, Riley DJ, Chen PL, Lee WH (May 2000). "Retinoblastoma protein enhances the fidelity of chromosome segregation mediated by hsHec1p". Molecular and Cellular Biology 20 (10): 3529–37. doi:10.1128/MCB.20.10.3529-3537.2000. PMC 85645. PMID 10779342.
Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH (Dec 2002). "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". The Journal of Biological Chemistry 277 (51): 49408–16. doi:10.1074/jbc.M207069200. PMID 12386167.
Tien AC, Lin MH, Su LJ, Hong YR, Cheng TS, Lee YC, Lin WJ, Still IH, Huang CY (Jan 2004). "Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model". Molecular & Cellular Proteomics 3 (1): 93–104. doi:10.1074/mcp.M300072-MCP200. PMID 14602875.
DeLuca JG, Howell BJ, Canman JC, Hickey JM, Fang G, Salmon ED (Dec 2003). "Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores". Current Biology 13 (23): 2103–9. doi:10.1016/j.cub.2003.10.056. PMID 14654001.
Bharadwaj R, Qi W, Yu H (Mar 2004). "Identification of two novel components of the human NDC80 kinetochore complex". The Journal of Biological Chemistry 279 (13): 13076–85. doi:10.1074/jbc.M310224200. PMID 14699129.
Lou Y, Yao J, Zereshki A, Dou Z, Ahmed K, Wang H, Hu J, Wang Y, Yao X (May 2004). "NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling". The Journal of Biological Chemistry 279 (19): 20049–57. doi:10.1074/jbc.M314205200. PMID 14978040.
Joseph J, Liu ST, Jablonski SA, Yen TJ, Dasso M (Apr 2004). "The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo". Current Biology 14 (7): 611–7. doi:10.1016/j.cub.2004.03.031. PMID 15062103.
Steensgaard P, Garrè M, Muradore I, Transidico P, Nigg EA, Kitagawa K, Earnshaw WC, Faretta M, Musacchio A (Jun 2004). "Sgt1 is required for human kinetochore assembly". EMBO Reports 5 (6): 626–31. doi:10.1038/sj.embor.7400154. PMC 1299074. PMID 15133482.
Stucke VM, Baumann C, Nigg EA (Aug 2004). "Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1". Chromosoma 113 (1): 1–15. doi:10.1007/s00412-004-0288-2. PMID 15235793.
Meraldi P, Draviam VM, Sorger PK (Jul 2004). "Timing and checkpoints in the regulation of mitotic progression". Developmental Cell 7 (1): 45–60. doi:10.1016/j.devcel.2004.06.006. PMID 15239953.
Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR, Oegema K, Desai A (Sep 2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension". Genes & Development 18 (18): 2255–68. doi:10.1101/gad.1234104. PMC 517519. PMID 15371340.
Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell Biology 6 (11): 1135–41. doi:10.1038/ncb1187. PMID 15502821.
Le XF, Lammayot A, Gold D, Lu Y, Mao W, Chang T, Patel A, Mills GB, Bast RC (Jan 2005). "Genes affecting the cell cycle, growth, maintenance, and drug sensitivity are preferentially regulated by anti-HER2 antibody through phosphatidylinositol 3-kinase-AKT signaling". The Journal of Biological Chemistry 280 (3): 2092–104. doi:10.1074/jbc.M403080200. PMID 15504738.
DeLuca JG, Dong Y, Hergert P, Strauss J, Hickey JM, Salmon ED, McEwen BF (Feb 2005). "Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites". Molecular Biology of the Cell 16 (2): 519–31. doi:10.1091/mbc.E04-09-0852. PMC 545888. PMID 15548592.

PDB gallery

2igp: Crystal Structure of Hec1 CH domain

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NDC80

Function

Interactions

References

Further reading