Lucifer yellow

Lucifer yellow
Names
IUPAC name
dilithium 6-Amino-2-(hydrazinecarbonyl)-1,3-dioxobenzo[de]isoquinoline-5,8-disulfonate
Identifiers
ChEBI CHEBI:52104 YesY
ChemSpider 20137740 N
Jmol interactive 3D Image
PubChem 20835957
Properties
C13H10Li2N4O9S2
Molar mass 444.24 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
N verify (what is YesYN ?)
Infobox references

Lucifer yellow is a fluorescent dye used in cell biology.[1] The key property of Lucifer yellow is that it can readily visualized in both living and fixed cells using a fluorescence microscope. Lucifer yellow was invented by Walter W. Stewart at the National Institutes of Health and patented in 1978.[2]

Preparations

For common usage it is compounded with carbohydrazide (CH) and prepared as a lithium salt. The CH group allows it to be covalently linked to surrounding biomolecules during aldehyde fixation.[3]

Other cations such as ammonium or potassium can be used when lithium is undesirable, but the resulting salts are less soluble in water.

Lucifer yellow can also be compounded as a vinyl sulfone, with ethylenediamine, or with cadaverine.

References

  1. Hanani, Menachem (January 2012). "Lucifer yellow – an angel rather than the devil". Journal of Cellular and Molecular Medicine 16 (2): 22–31. doi:10.1111/j.1582-4934.2011.01378.x. PMID 21740513.
  2. Patent description
  3. "Lucifer Yellow CH, Lithium Salt". Molecular Probes. Retrieved 17 March 2014.

External links

This article is issued from Wikipedia - version of the Monday, January 18, 2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.