LongSAGE

Long Serial Analysis of Gene Expression LongSAGE is a more robust version of the original protocol for SAGE.

Improvements over Previous Protocol

In the Serial Analysis of Gene Expression protocol developed in 1995, it was possible to use 5 μg of mRNA to generate a library of hundreds of cDNA tags.[1] The LongSAGE protocol developed in 2002 has a higher throughput, using 20 μg of mRNA to generate a cDNA library of thousands of tags.[2]

Notable Experiments

New transcription units in the mouse genome were identified using modified LongSAGE techniques.[3]

References

  1. Velculescu VE, Zhang L, Vogelstein B, Kinzler KW. (1995). "Serial analysis of gene expression". Science 270 (5235): 484–7. doi:10.1126/science.270.5235.484. PMID 7570003.
  2. Saha, S., et al. (2002). "Using the transcriptome to annotate the genome." Nat Biotechnol 20(5): 508-512.
  3. Wei, C. L., et al. (2004). "5 ' Long serial analysis of gene expression (LongSAGE) and 3 ' LongSAGE for transcriptome characterization and genome annotation."
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