Immobilized pH gradient

Immobilized pH gradient (IPG) gels are the acrylamide gel matrix co-polymerized with the pH gradient, which result in completely stable gradients except the most alkaline (>12) pH values. The immobilized pH gradient is obtained by the continuous change in the ratio of Immobilines. An Immobiline is a weak acid or base defined by its pK value.[1] This alternative method eliminates the problems of gradient instability and poor sample loading capacity associated with carrier ampholyte pH gradient.[2] Commercial precast IPG gels are available.

References

  1. ↑ Barnes et al., Electrophoresis in Practice: A Guide to theory and Practice. Page 44. VCH Publishers Inc, 1993 (USA)
  2. ↑ Wilkins et al., Proteome Research: New Frontiers in Functional Genomics. Page 14. Springer, 1997 (Germany)
This article is issued from Wikipedia - version of the Wednesday, August 06, 2014. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.