IRIS (biosensor)
Interferometric Reflectance Imaging Sensor (IRIS), formerly known as the Spectral Reflectance Imaging Biosensor (SRIB),[1] is a system that can be used as a biosensing platform capable of high-throughput multiplexing of protein-protein, protein-DNA, and DNA-DNA interactions without the use of any fluorescent labels. The sensing surface is prepared by robotic spotting of biological probes that are immobilized on functionalized Si/SiO2 substrates. IRIS is capable of quantifying bimolecular mass accumulated on the surface.[2]
Measurement
To perform a measurement, the sample is illuminated with multiple different wavelengths from either a tunable laser or different color LEDs; typically speaking, a relatively narrow bandwidth optical source. The reflection intensity is imaged using a CCD or CMOS camera. By using interferometric techniques, nanometer changes can be detected.
Applications
Applications for IRIS include microarray format immunoassays, single nucleotide polymorphism (SNP)[3] detection, pathogen detection and bio-defense monitoring, kinetic analysis of biomolecular interactions, and general biomolecular interaction studies for research applications.
References
- ↑ E. Ozkumur; et al. (2008). "Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications". Proceedings of the National Academy of Sciences 105 (23): 7988–7992. doi:10.1073/pnas.0711421105. PMC 2430348. PMID 18523019.
- ↑ E. Ozkumur; et al. (2009). "Quantification of DNA and protein adsorption by optical phase shift". Biosensors and Bioelectronics 25 (1): 167–172. doi:10.1016/j.bios.2009.06.033. PMC 2750076. PMID 19628383.
- ↑ E. Ozkumur; et al. (2010). "Label-free microarray imaging for direct detection of DNA hybridization and single-nucleotide mismatches". Biosensors and Bioelectronics 25 (7): 1789–1795. doi:10.1016/j.bios.2009.12.032. PMC 2824047. PMID 20097056.