EC50

The term half maximal effective concentration (EC50) refers to the concentration of a drug, antibody or toxicant which induces a response halfway between the baseline and maximum after a specified exposure time.[1] It is commonly used as a measure of drug's potency.

The EC50 of a graded dose response curve therefore represents the concentration of a compound where 50% of its maximal effect is observed.[2] The EC50 of a quantal dose response curve represents the concentration of a compound where 50% of the population exhibit a response,[3] after a specified exposure duration.

It is also related to IC50 which is a measure of a compound's inhibition (50% inhibition). For competition binding assays and functional antagonist assays IC50 is the most common summary measure of the dose-response curve. For agonist/stimulator assays the most common summary measure is the EC50.[4] Sometimes it is also expressed as pEC50 = - LOG(EC50) (with EC50 in M/l).

A small change in ligand concentration typically result in rapid changes in response in the biological system, following a sigmoidal function.The inflection point at which the increase in response with increasing ligand concentration begins to slow is the EC50. Which can be mathematically determined by derivation of the best-fit line. While relying on a graph for estimation is more convenient, this typical method yields less accurate results and less precise.[5]

Equation

Many different equations can be used to derive an EC50. One possible function is:

Y = Bottom + \frac{Top - Bottom}{1 + (\frac{X}{EC_{50}})^{\mathrm{-Hill coefficient}} }

where Y is the observed value, Bottom is the lowest observed value, Top is the highest observed value, and the Hill coefficient gives the largest absolute value of the slope of the curve.[6]

Limitations

The effects of a stressor or drug generally depend on the exposure time. Therefore, the EC50 (and similar statistics) will be a function of exposure time. The exact shape of this time function will depend upon the stressor (e.g., the specific toxicant), its mechanism of action, the organism exposed, etc. This time dependency hampers the comparison of potency or toxicity between compounds and between different organisms.

See also

References

  1. ↑ Introducing dose response curves, Graphpad Software
  2. ↑ EC50 definition
  3. ↑ definition of EC50 for quantal dose response curve
  4. ↑ Assay Operations for SAR Support NIH Chemical Genomics Center
  5. ↑ Assay Operations for SAR Support NIH Chemical Genomics Center
  6. ↑ EC50 equation - see page 5

External links

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