4-Hydroxy-3-methylbut-2-enyl diphosphate reductase

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase
Identifiers
EC number 1.17.1.2
CAS number 512789-14-9
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2, isopentenyl-diphosphate:NADP+ oxidoreductase, LytB, (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase, HMBPP reductase, IspH, LytB/IspH) is an enzyme in the non-mevalonate pathway. It acts upon (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate (or "HMB-PP").[1][2][3][4][5][6]

(1) isopentenyl diphosphate + NAD(P)+ + H2O \rightleftharpoons (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+
(2) dimethylallyl diphosphate + NAD(P)+ + H2O \rightleftharpoons (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase is an iron-sulfur protein that contains either a [3Fe-4S] or a [4Fe-4S] cluster.

References

  1. ↑ Rohdich, F., Hecht, S., Gärtner, K., Adam, P., Krieger, C., Amslinger, S., Arigoni, D., Bacher, A. and Eisenreich, W. (2002). "Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein". Proc. Natl. Acad. Sci. USA 99: 1158–1163. PMID 11818558.
  2. ↑ Hintz, M., Reichenberg, A., Altincicek, B., Bahr, U., Gschwind, R.M., Kollas, A.-K., Beck, E., Wiesner, J., Eberl, M. and Jomaa, H. (2001). "Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human T cells in Escherichia coli". FEBS Lett. 509: 317–322. PMID 11741609.
  3. ↑ Charon, L., Pale-Grosdemange, C. and Rohmer, M. (1999). "On the reduction steps in the mevalonate independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the bacterium Zymomonas mobilis". Tetrahedron Lett. 40: 7231–7234.
  4. ↑ Röhrich, R.C., Englert, N., Troschke, K., Reichenberg, A., Hintz, M., Seeber, F., Balconi, E., Aliverti, A., Zanetti, G., Köhler, U., Pfeiffer, M., Beck, E., Jomaa, H. and Wiesner, J. (2005). "Reconstitution of an apicoplast-localised electron transfer pathway involved in the isoprenoid biosynthesis of Plasmodium falciparum". FEBS Lett. 579: 6433–6438. PMID 16289098.
  5. ↑ Wolff, M., Seemann, M., Bui, T.S.B., Frapart, Y., Tritsch, D., Garcia Estrabot, A., Rodríguez-Concepción, M., Boronat, A., Marquet, A. and Rohmer, M. (2003). "Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein". FEBS Lett. 541: 115–120. PMID 12706830.
  6. ↑ Gräwert, T., Kaiser, J., Zepeck, F., Laupitz, R., Hecht, S., Amslinger, S., Schramek, N., Schleicher, E., Weber, S., Haslbeck, M., Buchner, J., Rieder, C., Arigoni, D., Bacher, A., Eisenreich, W. and Rohdich, F. (2004). "IspH protein of Escherichia coli: studies on iron-sulfur cluster implementation and catalysis". J. Am. Chem. Soc. 126: 12847–12855. PMID 15469281.
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