Genetically modified food

Genetically modified foods or GM foods, also genetically engineered foods, are foods produced from organisms that have had changes introduced into their DNA using the methods of genetic engineering. Genetic engineering techniques allow for the introduction of new traits as well as greater control over traits than previous methods such as selective breeding and mutation breeding.[1]

Commercial sale of genetically modified foods began in 1994, when Calgene first marketed its unsuccessful Flavr Savr delayed-ripening tomato.[2][3] Most food modifications have primarily focused on cash crops in high demand by farmers such as soybean, corn, canola, and cotton seed oil. Genetically modified crops have been engineered for resistance to pathogens and herbicides and for better nutrient profiles. GM livestock have been developed, although as of November 2013 none were on the market.[4]

There is general scientific agreement that food from genetically modified crops is not inherently riskier to human health than conventional food, but should be tested on a case-by-case basis.[5][6][7][8][9][10] However, there are ongoing public concerns related to food safety, regulation, labelling, environmental impact, research methods, and the fact that some GM seeds are subject to intellectual property rights owned by corporations.[11]

Definition

Genetically modified foods, GM foods or genetically engineered foods, are foods produced from organisms that have had changes introduced into their DNA using the methods of genetic engineering as opposed to traditional cross breeding.[12][13] In the US, the Department of Agriculture (USDA) and the Food and Drug Administration (FDA) favor the use of "genetic engineering" over "genetic modification" as the more precise term; the USDA defines genetic modification to include "genetic engineering or other more traditional methods."[14][15]

According to the World Health Organization, "Genetically modified organisms (GMOs) can be defined as organisms (i.e. plants, animals or microorganisms) in which the genetic material (DNA) has been altered in a way that does not occur naturally by mating and/or natural recombination. The technology is often called 'modern biotechnology' or 'gene technology', sometimes also 'recombinant DNA technology' or 'genetic engineering'. ... Foods produced from or using GM organisms are often referred to as GM foods."[12]

History

Human directed genetic manipulation of food began with the domestication of plants and animals through artificial selection at about 10,500 to 10,100 BC.[16]:1 The process of selective breeding, in which organisms with desired traits (and thus with the desired genes) are used to breed the next generation and organisms lacking the trait are not bred, is a precursor to the modern concept of genetic modification (GM).[16]:1[17]:1 With the discovery of DNA in the early 1900s and various advancements in genetic techniques through the 1970s[18] it became possible to directly alter the DNA and genes within food.

Genetically modified microbial enzymes were the first application of genetically modified organisms in food production and were approved in 1988 by the US Food and Drug Administration.[19] In the early 1990s, recombinant chymosin was approved for use in several countries.[19][20] Cheese had typically been made using the enzyme complex rennet that had been extracted from cows' stomach lining. Scientists modified bacteria to produce chymosin, which was also able to clot milk, resulting in cheese curds.[21]

The first genetically modified food approved for release was the Flavr Savr tomato in 1994.[2] Developed by Calgene, it was engineered to have a longer shelf life by inserting an antisense gene that delayed ripening.[22] In 1995, Bacillus thuringiensis (Bt) Potato was approved for cultivation, making it the first pesticide producing crop to be approved in the USA.[23] Other genetically modified crops receiving marketing approval in 1995 were: canola with modified oil composition, Bt maize, cotton resistant to the herbicide bromoxynil, Bt cotton, glyphosate-tolerant soybeans, virus-resistant squash, and another delayed ripening tomato.[2]

By 2010, 29 countries had planted commercialized biotech crops and a further 31 countries had granted regulatory approval for transgenic crops to be imported.[24] The US was the leading country in the production of GM foods in 2011, with twenty-five GM crops having received regulatory approval.[25] In 2015, 92% of corn, 94% of soybeans, and 94% of cotton produced in the US were genetically modified strains.[26]

With the creation of golden rice in 2000, scientists had genetically modified food to increase its nutrient value for the first time.[27]

The first genetically modified animal to be approved for food use was AquAdvantage salmon in 2015.[28] The salmon were transformed with a growth hormone-regulating gene from a Pacific Chinook salmon and a promoter from an ocean pout enabling it to grow year-round instead of only during spring and summer.[29]

The most widely planted GMOs are designed to tolerate herbicides. By 2006 some weed populations had evolved to tolerate some of the same herbicides. Palmer amaranth is a weed that competes with cotton. A native of the southwestern US, it traveled east and was first found resistant to glyphosate in 2006, less than 10 years after GM cotton was introduced.[30][31][32]

Process

Main article: Genetic engineering

Genetically engineered organisms are generated and tested in the laboratory for desired qualities. The most common modification is to add one or more genes to an organism's genome. Less commonly, genes are removed or their expression is increased or silenced or the number of copies of a gene is increased or decreased.

Once satisfactory strains are produced, the producer applies for regulatory approval to field-test them, called a "field release." Field-testing involves cultivating the plants on farm fields or growing animals in a controlled environment. If these field tests are successful, the producer applies for regulatory approval to grow and market the crop. Once approved, specimens (seeds, cuttings, breeding pairs, etc.) are cultivated and sold to farmers. The farmers cultivate and market the new strain. In some cases, the approval covers marketing but not cultivation.

According to the USDA, the number of field releases for genetically engineered organisms has grown from four in 1985 to an average of about 800 per year. Cumulatively, more than 17,000 releases had been approved through September 2013.[33]

Crops

Fruits and vegetables

3 views of the Sunset papaya cultivar, which was genetically modified to create the SunUp cultivar, resistant to PRSV.[34]

Papaya was genetically modified to resist the ringspot virus. 'SunUp' is a transgenic red-fleshed Sunset papaya cultivar that is homozygous for the coat protein gene PRSV; 'Rainbow' is a yellow-fleshed F1 hybrid developed by crossing 'SunUp' and nontransgenic yellow-fleshed 'Kapoho'.[34] The New York Times stated, "in the early 1990s, Hawaii’s papaya industry was facing disaster because of the deadly papaya ringspot virus. Its single-handed savior was a breed engineered to be resistant to the virus. Without it, the state’s papaya industry would have collapsed. Today, 80% of Hawaiian papaya is genetically engineered, and there is still no conventional or organic method to control ringspot virus."[35] The GM cultivar was approved in 1998.[36] In China, a transgenic PRSV-resistant papaya was developed by South China Agricultural University and was first approved for commercial planting in 2006; as of 2012 95% of the papaya grown in Guangdong province and 40% of the papaya grown in Hainan province was genetically modified.[37]

The New Leaf potato, brought to market by Monsanto in the late 1990s, was developed for the fast food market. It was withdrawn in 2001 after retailers rejected it and food processors ran into export problems.[38]

As of 2005, about 13% of the Zucchini (a form of squash) grown in the US was genetically modified to resist three viruses; that strain is also grown in Canada.[39][40]

Plums genetically engineered for resistance to plum pox, a disease carried by aphids.

In 2011, BASF requested the European Food Safety Authority's approval for cultivation and marketing of its Fortuna potato as feed and food. The potato was made resistant to late blight by adding resistant genes blb1 and blb2 that originate from the Mexican wild potato Solanum bulbocastanum.[41][42] In February 2013, BASF withdrew its application.[43]

In 2013, the USDA approved the import of a GM pineapple that is pink in color and that "overexpresses" a gene derived from tangerines and suppress other genes, increasing production of lycopene. The plant's flowering cycle was changed to provide for more uniform growth and quality. The fruit "does not have the ability to propagate and persist in the environment once they have been harvested," according to USDA APHIS. According to Del Monte's submission, the pineapples are commercially grown in a "monoculture" that prevents seed production, as the plant's flowers aren't exposed to compatible pollen sources. Importation into Hawaii is banned for "plant sanitation" reasons.[44]

In 2014, the USDA approved a genetically modified potato developed by J.R. Simplot Company that contained ten genetic modifications that prevent bruising and produce less acrylamide when fried. The modifications eliminate specific proteins from the potatoes, via RNA interference, rather than introducing novel proteins.[45][46]

In February 2015 Arctic Apples were approved by the USDA,[47] becoming the first genetically modified apple approved for sale in the US.[48] Gene silencing is used to reduce the expression of polyphenol oxidase (PPO), thus preventing the fruit from browning.[49]

Corn

Corn used for food and ethanol has been genetically modified to tolerate various herbicides and to express a protein from Bacillus thuringiensis (Bt) that kills certain insects.[50] About 90% of the corn grown in the U.S. was genetically modified in 2010.[51] In the US in 2015, 81% of corn acreage contained the Bt trait and 89% of corn acreage contained the glyphosate-tolerant trait.[26] Corn can be processed into grits, meal and flour as an ingredient in pancakes, muffins, doughnuts, breadings and batters, as well as baby foods, meat products, cereals and some fermented products. Corn-based masa flour and masa dough are used in the production of taco shells, corn chips and tortillas.[52]

Soy

Genetically modified soybean has been modified to tolerate herbicides, express Bt and produce healthier oils.[53] In 2015, 94% of soybean acreage in the U.S. was genetically modified to be glyphosate-tolerant.[26] Soybeans contain about 20% oil. In the most common method used to extract the oil, the soybeans are cracked, adjusted for moisture content, rolled into flakes and solvent-extracted with commercial hexane. The remaining soy meal has a 50% soy protein content. The meal is 'toasted' (actually heated with moist steam) and ground in a hammer mill. Part of the balance is processed further into high protein soy products that are used in a variety of foods, such as salad dressings, soups, meat analogues, beverage powders, cheeses, nondairy creamer, frozen desserts, whipped topping, infant formulas, breads, breakfast cereals, pasta and pet foods.[54][55] Processed soy protein appears in foods mainly in three forms: soy flour, soy protein isolates and soy protein concentrates.[55][56]

Food-grade soy protein isolate first became available on October 2, 1959.[57]:227–28 Soy protein isolate is a highly refined form of soy protein with a minimum protein content of 90% on a moisture-free basis. It is made from soy meal that has had most of the fats and carbohydrates removed. Soy isolates are mainly used to improve the texture of processed meat products and to increase protein content, enhance moisture retention and as an emulsifier.[58][59]

Soy protein concentrate is about 70% soy protein and is basically soybean meal without carbohydrates. Soy protein concentrate retains most of the bean fiber. It is used as a functional or nutritional ingredient in food products, mainly in baked foods, breakfast cereals and in some meat products. Soy protein concentrate is used in meat and poultry products to increase water and fat retention and to improve nutritional values (more protein, less fat).[58][60]

Soy flour is made by grinding soybeans into a fine powder. It comes in three forms: natural or full-fat (contains natural oils); defatted (oils removed) with 50% protein content and with either high water solubility or low water solubility; and lecithinated (lecithin added). As soy flour is gluten-free, yeast-raised breads made with soy flour are dense in texture. Soy grits are similar to soy flour except the soybeans have been toasted and cracked into coarse pieces. Kinako is a soy flour used in Japanese cuisine.[58][61]

Textured soy protein (TSP) is a fibrous, spongy matrix similar in texture to meat. TSP is used as a low-cost substitute in meat and poultry products.[58][62]

Derivative products

Corn starch and starch sugars, including syrups

Structure of the amylose molecule
Structure of the amylopectin molecule

Starch or amylum is a polysaccharide produced by all green plants as an energy store. Pure starch is a white, tasteless and odourless powder. It consists of two types of molecules: the linear and helical amylose and the branched amylopectin. Depending on the plant, starch generally contains 20 to 25% amylose and 75 to 80% amylopectin by weight.[63]

Starch can be further modified to create modified starch for specific purposes,[64] including creation of many of the sugars in processed foods. They include:

Lecithin

Lecithin is a naturally occurring lipid. It can be found in egg yolks and oil-producing plants. it is an emulsifier and thus is used in many foods. Corn, soy and safflower oil are sources of lecithin, though the majority of lecithin commercially available is derived from soy.[65][66][67][68] Sufficiently processed lecithin is often undetectable with standard testing practices.[63] According to the FDA, no evidence shows or suggests hazard to the public when lecithin is used at common levels. Lecithin added to foods amounts to only 2 to 10 percent of the 1 to 5 g of phosphoglycerides consumed daily on average.[65][66] Nonetheless, consumer concerns about GM food extend to such products.[69] This concern led to policy and regulatory changes in Europe in 2000, when Regulation (EC) 50/2000 was passed[70] which required labelling of food containing additives derived from GMOs, including lecithin. Because of the difficulty of detecting the origin of derivatives like lecithin with current testing practices, European regulations require those who wish to sell lecithin in Europe to employ a comprehensive system of Identity preservation (IP).[71][72]

Sugar

Structure of sucrose

The US imports 10% of its sugar, while the remaining 90% is extracted from sugar beet and sugarcane. After deregulation in 2005, glyphosate-resistant sugar beet was extensively adopted in the United States. 95% of beet acres in the US were planted with glyphosate-resistant seed in 2011.[73] Herbicide-tolerant beets are approved in Australia, Canada, Colombia, EU, Japan, Korea, Mexico, New Zealand, Philippines, Russian Federation and Singapore.[74] Pulp from the refining process is used as animal feed. The sugar produced from GM sugarbeets contains no DNA or protein—it is just sucrose that is chemically indistinguishable from sugar produced from non-GM sugarbeets.[63][75]

Independent analyses conducted by internationally recognized laboratories found that sugar from Roundup Ready sugar beets is identical to the sugar from comparably grown conventional (non-Roundup Ready) sugar beets. And, like all sugar, sugar from Roundup Ready sugar beets contains no genetic material or detectable protein (including the protein that provides glyphosate tolerance).[76]

Vegetable oil

Most vegetable oil used in the US is produced from GM crops canola,[77] corn,[67][78] cotton[79] and soybeans.[80] Vegetable oil is sold directly to consumers as cooking oil, shortening and margarine[81] and is used in prepared foods. There is a vanishingly small amount of protein or DNA from the original crop in vegetable oil.[63][82] Vegetable oil is made of triglycerides extracted from plants or seeds and then refined and may be further processed via hydrogenation to turn liquid oils into solids. The refining process[83] removes all, or nearly all non-triglyceride ingredients.[84]

Other uses

Animal feed

Livestock and poultry are raised on animal feed, much of which is composed of the leftovers from processing crops, including GM crops. For example, approximately 43% of a canola seed is oil. What remains after oil extraction is a meal that becomes an ingredient in animal feed and contains canola protein.[85] Likewise, the bulk of the soybean crop is grown for oil and meal. The high-protein defatted and toasted soy meal becomes livestock feed and dog food. 98% of the US soybean crop goes for livestock feed.[86][87] In 2011, 49% of the US maize harvest was used for livestock feed (including the percentage of waste from distillers grains).[88] "Despite methods that are becoming more and more sensitive, tests have not yet been able to establish a difference in the meat, milk, or eggs of animals depending on the type of feed they are fed. It is impossible to tell if an animal was fed GM soy just by looking at the resulting meat, dairy, or egg products. The only way to verify the presence of GMOs in animal feed is to analyze the origin of the feed itself."[89]

A 2012 literature review of studies evaluating the effect of GM feed on the health of animals did not find evidence that animals were adversely affected, although small biological differences were occasionally found. The studies included in the review ranged from 90 days to two years, with several of the longer studies considering reproductive and intergenerational effects.[90]

Proteins

Rennet is a mixture of enzymes used to coagulate milk into cheese. Originally it was available only from the fourth stomach of calves, and was scarce and expensive, or was available from microbial sources, which often produced unpleasant tastes. Genetic engineering made it possible to extract rennet-producing genes from animal stomachs and insert them into bacteria, fungi or yeasts to make them produce chymosin, the key enzyme.[91][92] The modified microorganism is killed after fermentation. Chymosin is isolated from the fermentation broth, so that the Fermentation-Produced Chymosin (FPC) used by cheese producers has an amino acid sequence that is identical to bovine rennet.[93] The majority of the applied chymosin is retained in the whey. Trace quantities of chymosin may remain in cheese.[93]

FPC was the first artificially produced enzyme to be approved by the US Food and Drug Administration.[19][20] FPC products have been on the market since 1990 and as of 2015 had yet to be surpassed in commercial markets.[94] In 1999, about 60% of US hard cheese was made with FPC.[95] Its global market share approached 80%.[96] By 2008, approximately 80% to 90% of commercially made cheeses in the US and Britain were made using FPC.[93] The most widely used FPC is produced either by the fungus Aspergillus niger (CHY-MAX®)[97][98]

In some countries, recombinant (GM) bovine somatotropin (also called rBST, or bovine growth hormone or BGH) is approved for administration to increase milk production. rBST may be present in milk from rBST treated cows, but it is destroyed in the digestive system and even if directly injected into the human bloodstream, has no observable effect on humans.[99][100][101] The FDA, World Health Organization, American Medical Association, American Dietetic Association and the National Institutes of Health have independently stated that dairy products and meat from rBST-treated cows are safe for human consumption.[102] However, on 30 September 2010, the United States Court of Appeals, Sixth Circuit, analyzing submitted evidence, found a "compositional difference" between milk from rBGH-treated cows and milk from untreated cows.[103][104] The court stated that milk from rBGH-treated cows has: increased levels of the hormone Insulin-like growth factor 1 (IGF-1); higher fat content and lower protein content when produced at certain points in the cow's lactation cycle; and more somatic cell counts, which may "make the milk turn sour more quickly."[104]

Livestock

A GM salmon, awaiting regulatory approval[105][106][107] since 1997,[108] was approved for human consumption by the American FDA in November 2015, to be raised in specific land-based hatcheries in Canada and Panama.[109]

A 2003 review published on behalf of Food Standards Australia New Zealand examined transgenic experimentation on terrestrial livestock species as well as aquatic species such as fish and shellfish. The review examined the molecular techniques used for experimentation as well as techniques for tracing the transgenes in animals and products as well as issues regarding transgene stability.[110]

Some mammals typically used for food production have been modified to produce non-food products, a practice sometimes called Pharming.

Recombinant food-grade organisms for healthcare

The use of genetically modified food-grade organisms as recombinant vaccine expression hosts and delivery vehicles can open new avenues for vaccinology. Considering that oral immunization is a beneficial approach in terms of costs, patient comfort, and protection of mucosal tissues, the use of food-grade organisms can lead to highly advantageous vaccines in terms of costs, easy administration, and safety. The organisms currently used for this purpose are bacteria (Lactobacillus and Bacillus), yeasts, algae, plants, and insect species. Several such organisms are under clinical evaluation, and the current adoption of this technology by the industry indicates a potential to benefit global healthcare systems.[111]

Health and safety

There is general scientific agreement that food on the market from genetically modified crops is not inherently riskier to human health than conventional food.[5][112][113] A 2004 report by the Institute of Medicine and National Research Council found that "genetic engineering is not an inherently hazardous process".[114] The report also stated "Adverse health effects from genetic engineering have not been documented in the human population, but the technique is new and concerns about its safety remain". The report stated that any method of producing new foods could lead to unwanted changes so that singling out genetic engineering is "scientifically unjustified," and called for case-by-case assessment for all novel foods.[114]

Opponents claim that long-term health risks have not been adequately assessed and propose various combinations of additional testing, labeling[115] or removal from the market.[116][117][118][119] The advocacy group European Network of Scientists for Social and Environmental Responsibility (ENSSER), disputes the claim that "science" supports the safety of current GM foods, proposing that each GM food must be judged on case-by-case basis.[120] The Canadian Association of Physicians for the Environment called for removing GM foods from the market pending long term health studies.[116] Multiple disputed studies have claimed health effects relating to GM foods or to the pesticides used with them.[121]

Testing

The requirements for safety testing of GMO food varies substantially between countries.[122] Countries such as the United States, Canada, Lebanon and Egypt use substantial equivalence to determine if further testing is required, while many countries such as those in the European Union, Brazil and China only authorize GMO cultivation on a case-by-case basis. In the U.S. the FDA determined that GMO's are "Generally Recognized as Safe" (GRAS) and therefore do not require additional testing if the GMO product is substantially equivalent to the non-modified product.[123] If new substances are found, further testing may be required to satisfy concerns over potential toxicity, allergenicity, possible gene transfer to humans or genetic outcrossing to other organisms.[7]

Regulation

Green: Mandatory labeling required; Red:Ban on import and cultivation of genetically engineered food.

Government regulation of GMO development and release varies widely between countries. Marked differences separate GMO regulation in the U.S. and GMO regulation in the European Union.[124] Regulation also varies depending on the intended product's use. For example, a crop not intended for food use is generally not reviewed by authorities responsible for food safety.[125]

United States Regulations

In the U.S., three government organizations regulate GMOs. The FDA checks the chemical composition of organisms for potential allergens. The United States Department of Agriculture (USDA) supervises field testing and monitors the distribution of GM seeds. The United States Environmental Protection Agency (EPA) is responsible for monitoring pesticide usage, including plants modified to contain proteins toxic to insects. Like USDA, EPA also oversees field testing and the distribution of crops that have had contact with pesticides to ensure environmental safety.[126] In 2015 the Obama administration announced that it would update the way the government regulated GM crops.[127]

In 1992 FDA published "Statement of Policy: Foods derived from New Plant Varieties." This statement is a clarification of FDA's interpretation of the Food, Drug, and Cosmetic Act with respect to foods produced from new plant varieties developed using recombinant deoxyribonucleic acid (rDNA) technology. FDA encouraged developers to consult with the FDA regarding any bioengineered foods in development. The FDA says developers routinely do reach out for consultations. In 1996 FDA updated consultation procedures.[128][129]

Labeling

As of 2015, 64 countries require labeling of GMO products in the marketplace.[130]

US and Canadian national policy is to require a label only given significant composition differences or documented health impacts, although some individual US states (Vermont, Connecticut and Maine) enacted laws requiring them.[131][132][133][134]

In some jurisdictions, the labeling requirement depends on the relative quantify of the GMF in the product. A study that investigated voluntary labeling in South Africa found that 31% of products labeled as GMO-free had a GM content above 1.0%.[135]

In Europe all food (including processed food) or feed that contains greater than 0.9% GMOs must be labelled.[136]

Detection

Testing on GMOs in food and feed is routinely done using molecular techniques such as PCR and bioinformatics.[137]

In a January 2010 paper, the extraction and detection of DNA along a complete industrial soybean oil processing chain was described to monitor the presence of Roundup Ready (RR) soybean: "The amplification of soybean lectin gene by end-point polymerase chain reaction (PCR) was successfully achieved in all the steps of extraction and refining processes, until the fully refined soybean oil. The amplification of RR soybean by PCR assays using event-specific primers was also achieved for all the extraction and refining steps, except for the intermediate steps of refining (neutralisation, washing and bleaching) possibly due to sample instability. The real-time PCR assays using specific probes confirmed all the results and proved that it is possible to detect and quantify genetically modified organisms in the fully refined soybean oil. To our knowledge, this has never been reported before and represents an important accomplishment regarding the traceability of genetically modified organisms in refined oils."[138]

According to Thomas Redick, detection and prevention of cross-pollination is possible through the suggestions offered by the Farm Service Agency (FSA) and Natural Resources Conservation Service (NRCS). Suggestions include educating farmers on the importance of coexistence, providing farmers with tools and incentives to promote coexistence, conduct research to understand and monitor gene flow, provide assurance of quality and diversity in crops, provide compensation for actual economic losses for farmers.[139]

Controversies

The genetically modified foods controversy consists of a set of disputes over the use of food made from genetically modified crops. The disputes involve consumers, farmers, biotechnology companies, governmental regulators, non-governmental organizations, environmental and political activists and scientists. The major disagreements include whether GM foods can be safely consumed, harm the environment and/or are adequately tested and regulated.[117][140] The objectivity of scientific research and publications has been challenged.[116] Farming-related disputes include the use and impact of pesticides, seed production and use, side effects on non-GMO crops/farms,[141] and potential control of the GM food supply by seed companies.[116]

The conflicts have continued since GM foods were invented. They have occupied the media, the courts, local, regional and national governments and international organizations.

See also

References

  1. ↑ GM Science Review First Report, Prepared by the UK GM Science Review panel (July 2003). Chairman Professor Sir David King, Chief Scientific Advisor to the UK Government, P 9
  2. 1 2 3 James, Clive (1996). "Global Review of the Field Testing and Commercialization of Transgenic Plants: 1986 to 1995" (PDF). The International Service for the Acquisition of Agri-biotech Applications. Retrieved 17 July 2010.
  3. ↑ Weasel, Lisa H. 2009. Food Fray. Amacom Publishing
  4. ↑ "Consumer Q&A". Fda.gov. 2009-03-06. Retrieved 2012-12-29.
  5. 1 2 American Association for the Advancement of Science (AAAS), Board of Directors (2012). Statement by the AAAS Board of Directors On Labeling of Genetically Modified Foods, and associated Press release: Legally Mandating GM Food Labels Could Mislead and Falsely Alarm Consumers
  6. ↑ American Medical Association (2012). Report 2 of the Council on Science and Public Health: Labeling of Bioengineered Foods
  7. 1 2 World Health Organization. Food safety: 20 questions on genetically modified foods. Accessed December 22, 2012.
  8. ↑ United States Institute of Medicine and National Research Council (2004). Safety of Genetically Engineered Foods: Approaches to Assessing Unintended Health Effects. National Academies Press. Free full-text. See pp11ff on need for better standards and tools to evaluate GM food.
  9. ↑ A decade of EU-funded GMO research (2001-2010) (PDF). Directorate-General for Research and Innovation. Biotechnologies, Agriculture, Food. European Union. 2010. p. 16. doi:10.2777/97784. ISBN 978-92-79-16344-9.
  10. ↑ Other sources:
  11. ↑ Cowan, Tadlock (18 Jun 2011). "Agricultural Biotechnology: Background and Recent Issues" (PDF). Congressional Research Service (Library of Congress). pp. 33–38. Retrieved 27 September 2015.
  12. 1 2 "Frequently asked questions on genetically modified foods". World Health Organization. Retrieved 29 September 2015.
  13. ↑ "Genetically engineered foods". University of Maryland Medical Center. Retrieved 29 September 2015.
  14. ↑ "Glossary of Agricultural Biotechnology Terms". United States Department of Agriculture. 27 Feb 2013. Retrieved 29 September 2015.
  15. ↑ "Questions & Answers on Food from Genetically Engineered Plants". US Food and Drug Administration. 22 Jun 2015. Retrieved 29 September 2015.
  16. 1 2 Daniel Zohary, Maria Hopf, Ehud Weiss (2012). Domestication of Plants in the Old World: The origin and spread of plants in the old world. Oxford University Press.
  17. ↑ Clive Root (2007). Domestication. Greenwood Publishing Groups.
  18. ↑ Jackson, DA; Symons, RH; Berg, P (1 October 1972). "Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing Lambda Phage Genes and the Galactose Operon of Escherichia coli". PNAS 69 (10): 2904–2909. Bibcode:1972PNAS...69.2904J. doi:10.1073/pnas.69.10.2904. PMC 389671. PMID 4342968.
  19. 1 2 3 "FDA Approves 1st Genetically Engineered Product for Food". Los Angeles Times. 24 March 1990. Retrieved 1 May 2014.
  20. 1 2 Staff, National Centre for Biotechnology Education, 2006. Case Study: Chymosin
  21. ↑ Campbell-Platt, Geoffrey (26 August 2011). Food Science and Technology. Ames, IA: John Wiley & Sons. ISBN 978-1-4443-5782-0.
  22. ↑ Bruening, G.; Lyons, J. M. (2000). "The case of the FLAVR SAVR tomato". California Agriculture 54 (4): 6–7. doi:10.3733/ca.v054n04p6.
  23. ↑ Genetically Altered Potato Ok'd For Crops Lawrence Journal-World - 6 May 1995
  24. ↑ Global Status of Commercialized Biotech/GM Crops: 2011 ISAAA Brief ISAAA Brief 43-2011. Retrieved 14 October 2012
  25. ↑ James, C (2011). "ISAAA Brief 43, Global Status of Commercialized Biotech/GM Crops: 2011". ISAAA Briefs. Ithaca, New York: International Service for the Acquisition of Agri-biotech Applications (ISAAA). Retrieved 2012-06-02.
  26. 1 2 3 "Adoption of Genetically Engineered Crops in the U.S.". Economic Research Service. USDA. Retrieved 26 August 2015.
  27. ↑ Ye, Xudong; Al-Babili, Salim; Klöti, Andreas; Zhang, Jing; Lucca, Paola; Beyer, Peter; Potrykus, Ingo (2000-01-14). "Engineering the Provitamin A (β-Carotene) Biosynthetic Pathway into (Carotenoid-Free) Rice Endosperm". Science 287 (5451): 303–305. doi:10.1126/science.287.5451.303. ISSN 0036-8075. PMID 10634784.
  28. ↑ "AQUABOUNTY CLEARED TO SELL SALMON IN USA FOR COMMERCIAL PURPOSES".
  29. ↑ Bodnar, Anastasia (October 2010). "Risk Assessment and Mitigation of AquAdvantage Salmon" (PDF). ISB News Report.
  30. ↑ Culpepper, Stanley A; et al. (2006). "Glyphosate-resistant Palmer amaranth (Amaranthus palmeri) confirmed in Georgia.". Weed Science 54 (4): 620–626. doi:10.1614/ws-06-001r.1.
  31. ↑ Gallant, Andre. "Pigweed in the Cotton: A superweed invades Georgia". Modern Farmer.
  32. ↑ Webster, TM; Grey, TL (2015). "Glyphosate-Resistant Palmer Amaranth (Amaranthus palmeri) Morphology, Growth, and Seed Production in Georgia.". Weed Science 63 (1): 264–272. doi:10.1614/ws-d-14-00051.1.
  33. ↑ Fernandez-Cornejo J, Wechsler S, Livingston M, Mitchell L (Feb 2014). "Genetically engineered crops in the United States". Economic Research Service.
  34. 1 2 Gonsalves, D. (2004). "Transgenic papaya in Hawaii and beyond". AgBioForum 7 (1&2): 36–40.
  35. ↑ Ronald, Pamela; McWilliams, James (May 14, 2010). "Genetically Engineered Distortions". The New York Times. Retrieved July 26, 2010.
  36. ↑ "The Rainbow Papaya Story". Hawaii Papaya Industry Association. Retrieved April 2015.
  37. ↑ Li, Y; et al. (April 2014). "Biosafety management and commercial use of genetically modified crops in China". Plant Cell Reports 33 (4): 565–73. doi:10.1007/s00299-014-1567-x. PMID 24493253.
  38. ↑ "The History and Future of GM Potatoes". Potatopro.com. 2010-03-10. Retrieved 2012-12-29.
  39. ↑ Johnson, Stanley R. (February 2008). "Quantification of the Impacts on US Agriculture of Biotechnology-Derived Crops Planted in 2006" (PDF). Washington DC: National Center for Food and Agricultural Policy. Retrieved August 12, 2010.
  40. ↑ "GMO Database: Zucchini (courgette)". GMO Compass. November 7, 2007. Retrieved February 28, 2015.
  41. ↑ "Business BASF applies for approval for another biotech potato". Research in Germany. November 17, 2011.
  42. ↑ Burger, Ludwig (October 31, 2011). "BASF applies for EU approval for Fortuna GM potato". Frankfurt: Reuters. Retrieved December 29, 2011.
  43. ↑ Turley, Andrew (February 7, 2013). "BASF drops GM potato projects". Royal Society of Chemistry News.
  44. ↑ PERKOWSKI, MATEUSZ (April 16, 2013). "Del Monte Gets Approval to Import GMO Pineapple". Food Democracy Now.
  45. ↑ Pollack, Andrew (November 7, 2014). "U.S.D.A. Approves Modified Potato. Next Up: French Fry Fans". The New York Times.
  46. ↑ "Availability of Petition for Determination of Nonregulated Status of Potato Genetically Engineered for Low Acrylamide Potential and Reduced Black Spot Bruise". Federal Register. May 3, 2013.
  47. ↑ Pollack, A. (February 13, 2015). "Gene-Altered Apples Get U.S. Approval". The New York Times.
  48. ↑ Tennille, Tracy (Feb 13, 2015). "First Genetically Modified Apple Approved for Sale in U.S.". Wall Street Journal. Retrieved Feb 2015.
  49. ↑ "Apple-to-apple transformation". Okanagan Specialty Fruits. Retrieved August 3, 2012.
  50. ↑ For a list of all traits, see table As of September 2012 that site listed 13 traits in nearly 30 different products.
  51. ↑ "Acreage NASS" (PDF). National Agricultural Statistics Board annual report. June 2010. Retrieved July 23, 2010.
  52. ↑ "Corn-Based Food Production in South Dakota: A Preliminary Feasibility Study" (PDF). South Dakota State University, College of Agriculture and Biological Sciences, Agricultural Experiment Station. June 2004.
  53. ↑ "GMO Compass - GM Soy".
  54. ↑ Lusas, Edmund W.; Riaz, Mian N (1995). "Soy Protein Products: Processing and Use" (PDF). 125 (3_Suppl). Journal of Nutrition: 573S–580S.
  55. 1 2 Sipos, E.S. "Edible Uses of Soybean Protein" (PDF).
  56. ↑ Singh, Preeti; Kumar, R.; Sabapathy, S. N.; Bawa, A. S. (2008). "Functional and Edible Uses of". Comprehensive Reviews in Food Science and Food Safety 7: 14–28. doi:10.1111/j.1541-4337.2007.00025.x.
  57. ↑ Shurtleff, William; Aoyagi, Akiko (2008). "History of Cooperative Soybean Processing in the United States: Extensively Annotated Bibliography and Sourcebook" (PDF). Soyinfo Center.
  58. 1 2 3 4 Weingartner, Karl; Owen, Bridget (March 2009). "Soy Protein Applications in Nutrition & Food Technology" (PDF). National Soybean Research Laboratory, University of Illinois at Urbana-Champaign.
  59. ↑ Isolated Soy Proteins
  60. ↑ Staff, World Initiative for Soy in Human Health (WISHH) Soy Protein Concentrate Reference Guide
  61. ↑ Soy Flours
  62. ↑ Textured Soy Proteins
  63. 1 2 3 4 Jaffe,Greg (Director of Biotechnology at the Center for Science in the Public Interest) (February 7, 2013). "What You Need to Know About Genetically Engineered Food". Atlantic.
  64. ↑ "International Starch: Production of corn starch". Starch.dk. Retrieved 2011-06-12.
  65. 1 2 "Lecithin". Oct 2015. Retrieved 18 October 2015.
  66. 1 2 "Select Committee on GRAS Substances (SCOGS) Opinion: Lecithin". Aug 10, 2015. Retrieved 18 October 2015.
  67. 1 2 "Poster of corn products" (PDF). Retrieved 2012-12-29.
  68. ↑ "Corn Oil, 5th Edition" (PDF). Corn Refiners Association. 2006.
  69. ↑ Staff (July 1, 2005). "Danisco emulsifier to substitute non-GM soy lecithin as demand outstrips supply". FoodNavigator.com.
  70. ↑ "Regulation (EC) 50/2000". Eur-lex.europa.eu.
  71. ↑ Marx,Gertruida M. (December 2010). "Dissertation submitted in fulfilment of requirements for the degree Doctor of Philosophy in the Faculty of Health Sciences" (PDF). MONITORING OF GENETICALLY MODIFIED FOOD PRODUCTS IN SOUTH AFRICA] (South Africa: University of the Free State).
  72. ↑ Davison, John; Bertheau, Yves Bertheau (2007). "EU regulations on the traceability and detection of GMOs: difficulties in interpretation, implementation and compliance". CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources 2 (77). doi:10.1079/pavsnnr20072077.
  73. ↑ "ISAAA Brief 43-2011. Executive Summary: Global Status of Commercialized Biotech/GM Crops: 2011". Isaaa.org. Retrieved 2012-12-29.
  74. ↑ "ISAAA Pocket K No. 2: Plant Products of Biotechnology". Isaaa.org. Retrieved 2012-12-29.
  75. ↑ Food and Agriculture Organization of the United Nations (2009). Sugar Beet: White Sugar (PDF). p. 9.
  76. ↑ Klein, Joachim; Altenbuchner, Josef; Mattes, Ralf (1998-02-26). "Nucleic acid and protein elimination during the sugar manufacturing process of conventional and transgenic sugar beets". Journal of Biotechnology 60 (3): 145–153. doi:10.1016/S0168-1656(98)00006-6. PMID 9608751.
  77. ↑ "Soyatech.com". Soyatech.com. Retrieved 2012-12-29.
  78. ↑ "Food Fats and Oils" (PDF). Institute of Shortening and Edible Oils. 2006. Retrieved 2011-11-19.
  79. ↑ "Twenty Facts about Cottonseed Oil". National Cottonseed Producers Association.
  80. ↑ Simon, Michelle (August 24, 2011). "ConAgra Sued Over GMO ’100% Natural’ Cooking Oils". Food Safety News.
  81. ↑ "ingredients of margarine". Imace.org. Retrieved 2012-12-29.
  82. ↑ "USDA Protein(g) in Fats and Oils". Retrieved 2015-05-31.
  83. ↑ "How Cooking Oil is Made". Madehow.com. 1991-04-27. Retrieved 2012-12-29.
  84. ↑ Crevel, R.W.R; Kerkhoff, M.A.T; Koning, M.M.G (2000). "Allergenicity of refined vegetable oils". Food and Chemical Toxicology 38 (4): 385–93. doi:10.1016/S0278-6915(99)00158-1. PMID 10722892.
  85. ↑ "What is Canola Oil?". CanolaInfo. Retrieved 2012-12-29.
  86. ↑ David Bennett for Southeast Farm Press, February 5, 2003 World soybean consumption quickens
  87. ↑ "Soybean". Encyclopedia Britannica Online. Retrieved February 18, 2012.
  88. ↑ "2012 World of Corn, National Corn Growers Association" (PDF). Retrieved 2012-12-29.
  89. ↑ Staff, GMO Compass. December 7, 2006. Genetic Engineering: Feeding the EU's Livestock
  90. ↑ Snell C; Bernheim A; Berge JB; Kuntz M; Pascal G; paris A; Ricroch AE (2012). "Assessment of the health impact of GM plant diets in long-term and multigenerational animal feeding trials: A literature review". Food and Chemical Toxicology 50 (3–4): 1134–1148. doi:10.1016/j.fct.2011.11.048. PMID 22155268.
  91. ↑ Emtage, JS; Angal, S; Doel, MT; Harris, TJ; Jenkins, B; Lilley, G; Lowe, PA (1983). "Synthesis of calf prochymosin (prorennin) in Escherichia coli". Proceedings of the National Academy of Sciences of the United States of America 80 (12): 3671–5. Bibcode:1983PNAS...80.3671E. doi:10.1073/pnas.80.12.3671. PMC 394112. PMID 6304731.
  92. ↑ Harris TJ, Lowe PA, Lyons A, Thomas PG, Eaton MA, Millican TA, Patel TP, Bose CC, Carey NH, Doel MT (April 1982). "Molecular cloning and nucleotide sequence of cDNA coding for calf preprochymosin". Nucleic Acids Res. 10 (7): 2177–87. doi:10.1093/nar/10.7.2177. PMC 320601. PMID 6283469.
  93. 1 2 3 "Chymosin". GMO Compass. Retrieved 2011-03-03.
  94. ↑ Law, Barry A. (2010). Technology of Cheesemaking. UK: WILEY-BLACKWELL. pp. 100–101. ISBN 978-1-4051-8298-0.
  95. ↑ "Food Biotechnology in the United States: Science, Regulation, and Issues". U.S. Department of State. Retrieved 2006-08-14.
  96. ↑ Johnson, M.E.; Lucey, J.A. (2006). "Major Technological Advances and Trends in Cheese". Journal of Dairy Science 89 (4): 1174–8. doi:10.3168/jds.S0022-0302(06)72186-5. PMID 16537950.
  97. ↑ Hansen, C. "Enzymes". Improving Food & Health. Retrieved 2014-01-14.
  98. ↑ "DMS cheese enzymes page".
  99. ↑ Baumana, Dale E.; Collier, Robert J (September 15, 2010). "Use of Bovine Somatotropin in Dairy Production" (PDF).
  100. ↑ Staff (2011-02-18). Last Medical Review. American Cancer Society. Missing or empty |title= (help);
  101. ↑ "Recombinant Bovine Growth Hormone".
  102. ↑ Brennand, Charlotte P. "Bovine Somatotropin in Milk" (PDF). Retrieved 2011-03-06.
  103. ↑ Cima, Greg (November 18, 2010). "Appellate court gives mixed ruling on Ohio rBST labeling rules". JAVMA News.
  104. 1 2 leafcom. "INTERNATIONAL DAIRY FOODS ASS'N v. BOGGS – Argued: June 10, 2010". Leagle.com.
  105. ↑ Rick MacInnes-Rae, Rick (November 27, 2013). "GMO salmon firm clears one hurdle but still waits for key OKs AquaBounty began seeking American approval in 1995". CBC News.
  106. ↑ Pollack, Andrew (May 21, 2012). "An Entrepreneur Bankrolls a Genetically Engineered Salmon". The New York Times. Retrieved September 3, 2012.
  107. ↑ Staff (December 26, 2012). "Draft Environmental Assessment and Preliminary Finding of No Significant Impact Concerning a Genetically Engineered Atlantic Salmon" (PDF). Federal Register. Retrieved January 2, 2013.
  108. ↑ Naik, Gautam (September 21, 2010). "Gene-Altered Fish Closer to Approval". Wall Street Journal.
  109. ↑ Commissioner, Office of the. "Press Announcements - FDA takes several actions involving genetically engineered plants and animals for food". www.fda.gov. Retrieved 2015-12-03.
  110. ↑ Harper, G.S., Brownlee, A., Hall, T.E., Seymour, R., Lyons, R. and Ledwith, P. (2003). "Global progress toward transgenic food animals: A survey of publicly available information." (PDF). Food Standards Australia and New Zealand. Retrieved August 27, 2015.
  111. ↑ Rosales-Mendoza, S.; Angulo, C.; Meza, B. (2015). "Food-Grade Organisms as Vaccine Biofactories and Oral Delivery Vehicles". Trends in Biotechnology. doi:10.1016/j.tibtech.2015.11.007.
  112. ↑ Ronald, Pamela (2011). "Plant Genetics, Sustainable Agriculture and Global Food Security". Genetics 188 (1): 11–20. doi:10.1534/genetics.111.128553. PMC 3120150. PMID 21546547.
  113. ↑ Bett, Charles; Ouma, James Okuro; Groote, Hugo De (August 2010). "Perspectives of gatekeepers in the Kenyan food industry towards genetically modified food". Food Policy 35 (4): 332–340. doi:10.1016/j.foodpol.2010.01.003.
  114. 1 2 "Composition of Altered Food Products, Not Method Used to Create Them, Should Be Basis for Federal Safety Assessment". National Academies of Sciences. Retrieved 2 January 2016.
  115. ↑ "Genetically modified foods" (PDF). Public Health Association of Australia. 2007.
  116. 1 2 3 4 "CAPE’s Position Statement on GMOs". Canadian Association of Physicians for the Environment. November 11, 2013.
  117. 1 2 "IDEA Position on Genetically Modified Foods". Irish Doctors’ Environmental Association. Retrieved 2014-03-25.
  118. ↑ "American Academy of Environmental Medicine Calls for Immediate Moratorium on Genetically Modified Foods, position paper". American Academy of Environmental Medicine. Retrieved 18 October 2015.
  119. ↑ "Press Advisory". American Academy of Environmental Medicine. Retrieved 18 October 2015.
  120. ↑ Hilbeck; et al. (2015). "No scientific consensus on GMO safety" (PDF). Environmental Sciences Europe 27. doi:10.1186/s12302-014-0034-1.
  121. ↑ Martinelli, L; et al. (2013). "Science, safety, and trust: the case of transgenic food". Croat Med J 54 (1): 91–6. doi:10.3325/cmj.2013.54.91. PMC 3584506. PMID 23444254.
  122. ↑ http://www.loc.gov/law/help/restrictions-on-gmos/
  123. ↑ Emily Marden, Risk and Regulation: U.S. Regulatory Policy on Genetically Modified Food and Agriculture 44 B.C.L. Rev. 733 (2003).
  124. ↑ http://www.cfr.org/agricultural-policy/regulation-gmos-europe-united-states-case-study-contemporary-european-regulatory-politics/p8688
  125. ↑ "The History and Future of GM Potatoes". PotatoPro.com.
  126. ↑ APPDMZ\ccvivr. "Commonly Asked Questions about the Food Safety of GMOs". monsanto.com.
  127. ↑ Pollack, Andrew (2015-07-02). "White House Orders Review of Rules for Genetically Modified Crops". The New York Times. ISSN 0362-4331. Retrieved 2015-07-03.
  128. ↑ "Food from Genetically Engineered Plants". FDA. Retrieved 18 October 2015.
  129. ↑ "Statement of Policy - Foods Derived from New Plant Varieties". Retrieved 18 October 2015.
  130. ↑ "International Labeling Laws". Center for Food Safety.]
  131. ↑ Chokshi, Niraj (9 May 2014). "Vermont just passed the nation’s first GMO food labeling law. Now it prepares to get sued". The Washington Post. Retrieved 19 January 2016.
  132. ↑ "The Regulation of Genetically Modified Food".
  133. ↑ Van Eenennaam, Alison; Chassy, Bruce; Kalaitzandonakes, Nicholas; Redick, Thomas (2014). "The Potential Impacts of Mandatory Labeling for Genetically Engineered Food in the United States" (PDF). Council for Agricultural Science and Technology (CAST) 54 (April 2014). ISSN 1070-0021. Retrieved 2014-05-28. To date, no material differences in composition or safety of commercialized GE crops have been identified that would justify a label based on the GE nature of the product.
  134. ↑ Hallenbeck, Terri (2014-04-27). "How GMO labeling came to pass in Vermont". Burlington Free Press. Retrieved 2014-05-28.
  135. ↑ Botha, Gerda M.; Viljoen, Christopher D. (2009). "South Africa: A case study for voluntary GM labelling". Food Chemistry 112 (4): 1060–4. doi:10.1016/j.foodchem.2008.06.050.
  136. ↑ Davison, John (2010). "GM plants: Science, politics and EC regulations". Plant Science 178 (2): 94–8. doi:10.1016/j.plantsci.2009.12.005.
  137. ↑ "EU GMO testing homepage". European Commission Join Research Centre. Retrieved May 31, 2015.
  138. ↑ Costa, Joana; Mafra, Isabel; Amaral, Joana S.; Oliveira, M.B.P.P. (2010). "Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques". Food Research International 43: 301–306. doi:10.1016/j.foodres.2009.10.003.
  139. ↑ "Mason Central Authentication Service". www.heinonline.org.mutex.gmu.edu. Retrieved 2015-12-15.
  140. ↑ American Medical Association (2012). Report 2 of the Council on Science and Public Health: Labeling of Bioengineered Foods. "To better detect potential harms of bioengineered foods, the Council believes that pre-market safety assessment should shift from a voluntary notification process to a mandatory requirement." page 7
  141. ↑ Chartered Institute of Environmental Health (2006) Proposals for managing the coexistence of GM, conventional and organic crops Response to the Department for Environment, Food and Rural Affairs consultation paper. October 2006

External links

This article is issued from Wikipedia - version of the Saturday, February 13, 2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.