Formate dehydrogenase (NADP+)

In enzymology, a formate dehydrogenase (NADP+) (EC 1.2.1.43) is an enzyme that catalyzes the chemical reaction

formate + NADP⁺ CO₂ + NADPH

Thus, the two substrates of this enzyme are formate and NADP⁺, whereas its two products are CO₂ and NADPH.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is formate:NADP+ oxidoreductase. Other names in common use include NADP+-dependent formate dehydrogenase, and formate dehydrogenase (NADP+). This enzyme participates in methane metabolism. It has 3 cofactors: iron, Tungsten, and Selenium.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 2GSD.

References

• Andreesen JR, Ljungdahl LG (1974). "Nicotinamide adenine dinucleotide phosphate-dependent formate dehydrogenase from Clostridium thermoaceticum: purification and properties". J. Bacteriol. 120 (1): 6–14. PMC 245723. PMID 4154039. 
• Yamamoto I, Saiki T, Liu SM, Ljungdahl LG (1983). "Purification and properties of NADP-dependent formate dehydrogenase from Clostridium thermoaceticum, a tungsten-selenium-iron protein". J. Biol. Chem. 258 (3): 1826–32. PMID 6822536.