David Klenerman

David Klenerman
Nationality British
Fields Biophysical chemistry
Institutions University of Cambridge
Stanford University
Alma mater University of Cambridge
Doctoral advisor I W M Smith FRS
Other academic advisors Richard Zare
Known for Illumina dye sequencing,
Scanning ion-conductance microscopy,
Super-resolution microscopy,
Solexa
Notable awards
Website
www.klenermangroup.co.uk

David Klenerman FRS[1] FMedSci[2] is a British biophysical chemist. Currently, he is a Professor of Biophysical Chemistry at the Department of Chemistry of the University of Cambridge[4] and a fellow of the Christ's College, Cambridge.[5] He is best known for his contribution in the field of Next-generation sequencing of DNA (that subsequently resulted Solexa, a high-speed DNA sequencing company that he co-founded),[6][7][8][9] Nanopipette based Scanning ion-conductance microscopy,[10][11][12] and super-resolution microscopy.[13][14]

Education and Career

Klenerman attended Christ's College, Cambridge and received his bachelor's degree in 1982.[15] He earned his Doctor of Philosophy in Chemistry in 1986 from Churchill College, Cambridge where he worked with I W M Smith FRS.[3][15] He then went to Stanford University as a Fullbright Scholar to work on high-overtone chemistry, with Richard Zare. After his postdoctoral stint at Stanford, he returned to United Kingdom to work in BP Research for seven years. Then, in 1994, he joined the University of Cambridge, as a faculty member of the Department of Chemistry and a fellow of Christ's college.[3][15][16]

Research

Klenerman, along with Shankar Balasubramanian, invented a method of next-generation DNA Sequencing which is commonly known today as the Solexa sequencing or Illumina dye sequencing.[6][8] The method is based on the detection of fluorophore labelled nucleotides as they get incorporated in the DNA strands.[17] This sequencing by synthesis method gained popularity,[18][19] and is currently regarded as the most widely used platform to replace conventional Sanger sequencing technique, despite its comparatively low multiplexing capability of samples, as it offers several key advantages: it is automated, quick, highly accurate, capable of sequencing multiple strands simultaneously via Massive parallel sequencing, and economically cheaper in case of whole genome sequencing.[20][21][22]

He is also known for exploring nanopipette-based (instead of conventional micropipette-based) scanning ion-conductance microscopy methods.[10][23] His research group was successful in achieving very high resolution topographic images of live-cells, in hopping mode imaging, in precise delivery of small molecules to cell, and in studying real time detailed cell-functioning.[11][12][24][25][26]

Most recently, his group is focusing on 3D super-resolution microscopy to develop new insights on protein misfolding and neurodegenerative diseases.[13][14][27]

Awards and honours

The major awards and honours that Klenerman received in recognition of his research work:

Companies

Klenerman and Balasubramanian commercialized their invention on the single-molecule-fluorescence based high-speed DNA sequencing and jointly founded Solexa in 1998. Later, in 2007, this company was acquired by Illumina for $600 million.[6][29][30][31][32]

In 2004, Klenerman co-founded another spin-out company, Ionscope, to supply assembled scanning ion-conductance microscopes to the research community that looks for high-resolution 3D images of live cells. As per the Biotechnology and Biological Sciences Research Council, as of February 2014, Ionscope sold 35 SICM units worldwide.[33]

References

  1. 1 2 3 "David Klenerman : Royal Society". Fellows Directory. Royal Society. Retrieved 26 January 2016.
  2. 1 2 3 "Fellow : Academy of Medical Sciences". Academy of Medical Sciences, United Kingdom. Retrieved 26 January 2016.
  3. 1 2 3 4 "Interdisciplinary Award 2007 Winner". Royal Society of Chemistry. Retrieved 26 January 2016.
  4. ↑ "Professor David Klenerman FMedSci FRS". Department of Chemistry, University of Cambridge. University of Cambridge. Retrieved 26 January 2016.
  5. ↑ "Professor David Klenerman". Christ's College, Cambridge. Retrieved 26 January 2016.
  6. 1 2 3 "History of Illumina Sequencing". Illumina (company). Retrieved 26 January 2016.
  7. ↑ Davies, Kevin (7 September 2010). The $1,000 Genome. Free Press (publisher). pp. 102–115. ISBN 1416569596. Retrieved 26 January 2016.
  8. 1 2 "Accurate whole human genome sequencing using reversible terminator chemistry". Nature 456 (7218). 6 November 2008. doi:10.1038/nature07517. Retrieved 26 January 2016.
  9. ↑ "The Solexa Story". Bio-IT World (September–October 2010). 28 September 2010. Retrieved 26 January 2016.
  10. 1 2 "Nanodoodling shows pipette power". BBC News. 5 September 2006. Retrieved 26 January 2016.
  11. 1 2 "Two-Tone Molecular Printing". Phys.org. 18 October 2005. Retrieved 26 January 2016.
  12. 1 2 "Drawing With DNA: Nanopipette allows voltage-controlled delivery of biomolecules to a surface". Chemical & Engineering News (Volume 83, Number 44) (American Chemical Society). 31 October 2005. Retrieved 26 January 2016.
  13. 1 2 "The super-resolution revolution". Phys.org. 27 February 2015. Retrieved 26 January 2016.
  14. 1 2 "The 2015 super-resolution microscopy roadmap". Journal of Physics D 48 (44). 14 October 2015. doi:10.1088/0022-3727/48/44/443001. Retrieved 26 January 2016.
  15. 1 2 3 "KLENERMAN, Prof. David". Who's Who (UK). A & C Black, Oxford University Press. Retrieved 26 January 2016. (subscription required (help)).
  16. ↑ "David Klenerman elected FRS". Christ's College, Cambridge. Retrieved 27 January 2016.
  17. ↑ Balasubramanian, Shankar (4 May 2011). "Sequencing nucleic acids: from chemistry to medicine". Chemical Communications 47: 7281–7286. doi:10.1039/c1cc11078k. Retrieved 29 January 2016.
  18. ↑ Quail, Michael A (25 November 2008). "A large genome center’s improvements to the Illumina sequencing system". Nature Methods 5: 1005–1010. doi:10.1038/nmeth.1270. Retrieved 29 January 2016.
  19. ↑ Cronn, Richard (27 August 2008). "Multiplex sequencing of plant chloroplast genomes using Solexa sequencing-by-synthesis technology". Nucleic Acids Research 36 (19). doi:10.1093/nar/gkn502. Retrieved 29 January 2016.
  20. ↑ Metzker, Michael L. (8 December 2009). "Sequencing technologies — the next generation". Nature Reviews Genetics 11 (1): 31–46. doi:10.1038/nrg2626. Retrieved 29 January 2016.
  21. ↑ Pettersson, Erik; Lundeberg, Joakim; Ahmadian, Afshin (2009). "Generations of sequencing technologies". Genomics 93: 105–111. doi:10.1016/j.ygeno.2008.10.003. Retrieved 29 January 2016.
  22. ↑ Quail, Michael A (24 July 2012). "A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers". BMC Genomics 13 (341). doi:10.1186/1471-2164-13-341. PMID 22827831. Retrieved 29 January 2016.
  23. ↑ Shevchuk, Andrew I (2011). "Realizing the biological and biomedical potential of nanoscale imaging using a pipette probe". Nanomedicine 6 (3). doi:10.2217/nnm.10.154. Retrieved 29 January 2016.
  24. ↑ "Nano World: Twin-barreled nano-eyedropper". Phys.org. 7 March 2006. Retrieved 29 January 2016.
  25. ↑ "Scanning Ion Conductance Microscopy". Annual Review of Analytical Chemistry 5 (207): 207–228. 2012. doi:10.1146/annurev-anchem-062011-143203. Retrieved 29 January 2016.
  26. ↑ "Recent advances in high resolution scanning electrochemical microscopy of living cells – A review". Analytica Chimica Acta 775. 2 May 2013. doi:10.1016/j.aca.2012.12.042. Retrieved 29 January 2016.
  27. ↑ "Physical sciences illuminate neurodegenerative diseases". Medical Xpress (Phys.org). 29 May 2012. Retrieved 29 January 2016.
  28. ↑ "British Biophysical Society Lecture Tour". University College Dublin. Retrieved 27 January 2016.
  29. ↑ "Cutting the UK science budget would be a false economy". Financial Times (The Nikkei). 18 June 2013. Retrieved 27 January 2016.
  30. ↑ "$600m for DNA firm". Varsity. Retrieved 27 January 2016.
  31. ↑ "Illumina Buys Solexa". Forbes. 13 November 2006. Retrieved 27 January 2016.
  32. ↑ "Illumina to Buy Solexa For $600 Million in Stock". The Wall Street Journal. 13 November 2006. Retrieved 27 January 2016.
  33. ↑ "New microscope technology enables imaging of live cells". BBSRC. Retrieved 30 January 2016.

External links

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