Eagle's minimal essential medium
Eagle's minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture.
It contains:
- amino acids
- salts (calcium chloride, potassium chloride, magnesium sulfate, sodium chloride, and monosodium phosphate)
- glucose
- vitamins (folic acid, nicotinamide, riboflavin, B12)
A variation of this medium, called Dulbecco’s modified Eagle's medium (DMEM), (Dulbecco/Vogt modified Eagle's minimal essential medium), contains approximately four times as much of the vitamins and amino acids present in the original formula and two to four times as much glucose. Additionally, it contains iron and phenol red. DMEM is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish[1] cells.
See also
- RPMI (Roswell Park Memorial Institute medium), for lymph cells
- GMEM (Glasgow's Minimal Essential Medium)
References
- ↑ Pombinho AR, Laizé V, Molha DM, Marques SMP, Cancela ML (March 2004). "Development of two bone-derived cell lines from the marine teleost Sparus aurata; evidence for extracellular matrix mineralization and cell-type-specific expression of matrix Gla protein and osteocalcin". Cell and Tissue Research 315 (3): 393–406. doi:10.1007/s00441-003-0830-1. PMID 14762709.
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