DDEF1

ArfGAP with SH3 domain, ankyrin repeat and PH domain 1

PDB rendering based on 2da0.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
2D1X, 2DA0, 2ED1, 2RQT, 2RQU

Identifiers

Symbols

ASAP1 ; AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P

External IDs

OMIM: 605953 MGI: 1342335 HomoloGene: 7684 GeneCards: ASAP1 Gene

Gene ontology
Molecular function	• GTPase activator activity • protein binding • phosphatidylinositol-4,5-bisphosphate binding • phosphatidylinositol-3,4,5-trisphosphate binding • metal ion binding
Cellular component	• cytosol • membrane
Biological process	• organelle organization • positive regulation of GTPase activity • cilium morphogenesis
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 8:
130.05 – 130.44 Mb

Chr 15:
64.09 – 64.38 Mb

PubMed search

Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 is a protein that in humans is encoded by the ASAP1 gene.^[1]^[2]

Interactions

DDEF1 has been shown to interact with Src.^[3]

References

↑ Brown MT, Andrade J, Radhakrishna H, Donaldson JG, Cooper JA, Randazzo PA (Dec 1998). "ASAP1, a phospholipid-dependent arf GTPase-activating protein that associates with and is phosphorylated by Src". Mol Cell Biol 18 (12): 7038–51. PMC 109286. PMID 9819391.
↑ "Entrez Gene: DDEF1 development and differentiation enhancing factor 1".
↑ Brown, M T; Andrade J; Radhakrishna H; Donaldson J G; Cooper J A; Randazzo P A (Dec 1998). "ASAP1, a phospholipid-dependent arf GTPase-activating protein that associates with and is phosphorylated by Src". Mol. Cell. Biol. (UNITED STATES) 18 (12): 7038–51. ISSN 0270-7306. PMC 109286. PMID 9819391.

Nagase T, Ishikawa K, Kikuno R; et al. (2000). "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.". DNA Res. 6 (5): 337–45. doi:10.1093/dnares/6.5.337. PMID 10574462.
Furman C, Short SM, Subramanian RR; et al. (2002). "DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism.". J. Biol. Chem. 277 (10): 7962–9. doi:10.1074/jbc.M109149200. PMID 11773070.
Scott MP, Zappacosta F, Kim EY; et al. (2002). "Identification of novel SH3 domain ligands for the Src family kinase Hck. Wiskott-Aldrich syndrome protein (WASP), WASP-interacting protein (WIP), and ELMO1.". J. Biol. Chem. 277 (31): 28238–46. doi:10.1074/jbc.M202783200. PMID 12029088.
Liu Y, Loijens JC, Martin KH; et al. (2002). "The association of ASAP1, an ADP ribosylation factor-GTPase activating protein, with focal adhesion kinase contributes to the process of focal adhesion assembly.". Mol. Biol. Cell 13 (6): 2147–56. doi:10.1091/mbc.E02-01-0018. PMC 117631. PMID 12058076.
Oshiro T, Koyama S, Sugiyama S; et al. (2002). "Interaction of POB1, a downstream molecule of small G protein Ral, with PAG2, a paxillin-binding protein, is involved in cell migration.". J. Biol. Chem. 277 (41): 38618–26. doi:10.1074/jbc.M203453200. PMID 12149250.
Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Oda A, Wada I, Miura K; et al. (2003). "CrkL directs ASAP1 to peripheral focal adhesions.". J. Biol. Chem. 278 (8): 6456–60. doi:10.1074/jbc.M210817200. PMID 12522101.
Ota T, Suzuki Y, Nishikawa T; et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Onodera Y, Hashimoto S, Hashimoto A; et al. (2005). "Expression of AMAP1, an ArfGAP, provides novel targets to inhibit breast cancer invasive activities.". EMBO J. 24 (5): 963–73. doi:10.1038/sj.emboj.7600588. PMC 554134. PMID 15719014.
Ehlers JP, Worley L, Onken MD, Harbour JW (2005). "DDEF1 is located in an amplified region of chromosome 8q and is overexpressed in uveal melanoma.". Clin. Cancer Res. 11 (10): 3609–13. doi:10.1158/1078-0432.CCR-04-1941. PMID 15897555.
Che MM, Boja ES, Yoon HY; et al. (2005). "Regulation of ASAP1 by phospholipids is dependent on the interface between the PH and Arf GAP domains.". Cell. Signal. 17 (10): 1276–88. doi:10.1016/j.cellsig.2005.01.007. PMID 16038802.
Nusbaum C, Mikkelsen TS, Zody MC; et al. (2006). "DNA sequence and analysis of human chromosome 8.". Nature 439 (7074): 331–5. doi:10.1038/nature04406. PMID 16421571.
Hashimoto S, Hirose M, Hashimoto A; et al. (2006). "Targeting AMAP1 and cortactin binding bearing an atypical src homology 3/proline interface for prevention of breast cancer invasion and metastasis.". Proc. Natl. Acad. Sci. U.S.A. 103 (18): 7036–41. doi:10.1073/pnas.0509166103. PMC 1459014. PMID 16636290.

PDB gallery

2da0: Solution structure of the PH domain of PIP2-dependent ARF1 GTPase-activating protein from human

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DDEF1

Interactions

References

Further reading