Cresol Red

Cresol Red
Identifiers


CAS Number	1733-12-6^Y
ChEBI	CHEBI:86218^N
ChemSpider	65818^Y
Jmol interactive 3D	Image Image
PubChem	73013
InChI InChI=1S/C21H18O5S/c1-13-11-15(7-9-18(13)22)21(16-8-10-19(23)14(2)12-16)17-5-3-4-6-20(17)27(24,25)26-21/h3-12,22-23H,1-2H3^Y Key: OBRMNDMBJQTZHV-UHFFFAOYSA-N^Y InChI=1/C21H18O5S/c1-13-11-15(7-9-18(13)22)21(16-8-10-19(23)14(2)12-16)17-5-3-4-6-20(17)27(24,25)26-21/h3-12,22-23H,1-2H3 Key: OBRMNDMBJQTZHV-UHFFFAOYAD
SMILES Cc1cc(ccc1O)/C(=C/2C=CC(=O)C(=C2)C)c3ccccc3OS(O)=O O=S2(=O)OC(c1ccccc12)(c3ccc(O)c(c3)C)c4ccc(O)c(c4)C
Properties
Chemical formula	C₂₁H₁₇NaO₅S
Molar mass	382.43 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
N verify (what is ^YN ?)
Infobox references

Cresol Red (full name: o-Cresolsulfonephthalein) is a triarylmethane dye frequently used for monitoring the pH in aquaria.

pH indicator

Cresol Red (pH indicator)
below pH 7.2		above pH 8.8
7.2	⇌	8.8

Molecular biology

Cresol Red can be used in many common molecular biology reactions in place of other loading dyes. Cresol Red does not inhibit Taq polymerase to the same degree as other common loading dyes.

Color marker

Cresol Red can also be used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. In a 1% agarose gel, it runs approximately at the size of a 125 base pair (bp) DNA molecule (it depends on the concentration of buffer and other component). Bromophenol blue and xylene cyanol can also be used for this purpose.

References

External links

Cresol Red at OpenWetWare life scientists' wiki

This article is issued from Wikipedia - version of the Monday, June 29, 2015. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.