CRSP3

Mediator complex subunit 23

Identifiers

MED23 ; ARC130; CRSP130; CRSP133; CRSP3; DRIP130; MRT18; SUR-2; SUR2

External IDs

OMIM: 605042 MGI: 1917458 HomoloGene: 3552 ChEMBL: 4146 GeneCards: MED23 Gene

Gene ontology
Molecular function	• transcription coactivator activity • protein binding • ubiquitin protein ligase activity
Cellular component	• ubiquitin ligase complex • nucleoplasm • transcription factor complex • mediator complex
Biological process	• regulation of transcription, DNA-templated • regulation of transcription from RNA polymerase II promoter • transcription initiation from RNA polymerase II promoter • gene expression • protein ubiquitination
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 6:
131.57 – 131.63 Mb

Chr 10:
24.87 – 24.91 Mb

PubMed search

Mediator of RNA polymerase II transcription subunit 23 is an enzyme that in humans is encoded by the MED23 gene.^[1]^[2]

Function

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.^[2]

Interactions

CRSP3 has been shown to interact with Estrogen receptor alpha,^[3] CEBPB^[4] and Cyclin-dependent kinase 8.^[3]^[5]

References

↑ Ryu S, Zhou S, Ladurner AG, Tjian R (Feb 1999). "The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1". Nature 397 (6718): 446–50. doi:10.1038/17141. PMID 9989412.
1 2 "Entrez Gene: CRSP3 cofactor required for Sp1 transcriptional activation, subunit 3, 130kDa".
1 2 Kang YK, Guermah M, Yuan CX, Roeder RG (Mar 2002). "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro". Proceedings of the National Academy of Sciences of the United States of America 99 (5): 2642–7. doi:10.1073/pnas.261715899. PMC 122401. PMID 11867769.
↑ Mo X, Kowenz-Leutz E, Xu H, Leutz A (Jan 2004). "Ras induces mediator complex exchange on C/EBP beta". Molecular Cell 13 (2): 241–50. doi:10.1016/S1097-2765(03)00521-5. PMID 14759369.
↑ Wang G, Cantin GT, Stevens JL, Berk AJ (Jul 2001). "Characterization of mediator complexes from HeLa cell nuclear extract". Molecular and Cellular Biology 21 (14): 4604–13. doi:10.1128/MCB.21.14.4604-4613.2001. PMC 87123. PMID 11416138.

Rachez C, Lemon BD, Suldan Z, Bromleigh V, Gamble M, Näär AM, Erdjument-Bromage H, Tempst P, Freedman LP (Apr 1999). "Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex". Nature 398 (6730): 824–8. doi:10.1038/19783. PMID 10235266.
Näär AM, Beaurang PA, Zhou S, Abraham S, Solomon W, Tjian R (Apr 1999). "Composite co-activator ARC mediates chromatin-directed transcriptional activation". Nature 398 (6730): 828–32. doi:10.1038/19789. PMID 10235267.
Boyer TG, Martin ME, Lees E, Ricciardi RP, Berk AJ (May 1999). "Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein". Nature 399 (6733): 276–9. doi:10.1038/20466. PMID 10353252.
Nagase T, Ishikawa K, Kikuno R, Hirosawa M, Nomura N, Ohara O (Oct 1999). "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research 6 (5): 337–45. doi:10.1093/dnares/6.5.337. PMID 10574462.
Ko L, Cardona GR, Chin WW (May 2000). "Thyroid hormone receptor-binding protein, an LXXLL motif-containing protein, functions as a general coactivator". Proceedings of the National Academy of Sciences of the United States of America 97 (11): 6212–7. doi:10.1073/pnas.97.11.6212. PMC 18584. PMID 10823961.
Hartley JL, Temple GF, Brasch MA (Nov 2000). "DNA cloning using in vitro site-specific recombination". Genome Research 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (Mar 2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
Stevens JL, Cantin GT, Wang G, Shevchenko A, Shevchenko A, Berk AJ (Apr 2002). "Transcription control by E1A and MAP kinase pathway via Sur2 mediator subunit". Science 296 (5568): 755–8. doi:10.1126/science.1068943. PMID 11934987.
Asada S, Choi Y, Yamada M, Wang SC, Hung MC, Qin J, Uesugi M (Oct 2002). "External control of Her2 expression and cancer cell growth by targeting a Ras-linked coactivator". Proceedings of the National Academy of Sciences of the United States of America 99 (20): 12747–52. doi:10.1073/pnas.202162199. PMC 130531. PMID 12242338.
Nakayama M, Kikuno R, Ohara O (Nov 2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Research 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
Goldberg SF, Miele ME, Hatta N, Takata M, Paquette-Straub C, Freedman LP, Welch DR (Jan 2003). "Melanoma metastasis suppression by chromosome 6: evidence for a pathway regulated by CRSP3 and TXNIP". Cancer Research 63 (2): 432–40. PMID 12543799.
Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower CS, Conaway RC, Conaway JW (Dec 2003). "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex". The Journal of Biological Chemistry 278 (50): 49671–4. doi:10.1074/jbc.C300444200. PMID 14576168.
Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW (Feb 2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2". The Journal of Biological Chemistry 279 (7): 5846–51. doi:10.1074/jbc.M312523200. PMID 14638676.
Mo X, Kowenz-Leutz E, Xu H, Leutz A (Jan 2004). "Ras induces mediator complex exchange on C/EBP beta". Molecular Cell 13 (2): 241–50. doi:10.1016/S1097-2765(03)00521-5. PMID 14759369.
Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Molecular Cell 14 (5): 685–91. doi:10.1016/j.molcel.2004.05.006. PMID 15175163.
Wada O, Oishi H, Takada I, Yanagisawa J, Yano T, Kato S (Aug 2004). "BRCA1 function mediates a TRAP/DRIP complex through direct interaction with TRAP220". Oncogene 23 (35): 6000–5. doi:10.1038/sj.onc.1207786. PMID 15208681.
Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A (Oct 2004). "From ORFeome to biology: a functional genomics pipeline". Genome Research 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.

This article is issued from Wikipedia - version of the Sunday, August 02, 2015. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.

CRSP3

Function

Interactions

References

Further reading