Aniline Blue WS

Water blue
Methyl blue

Aniline Blue WS, also called aniline blue, China blue, or Soluble blue, is a mixture of methyl blue and water blue. It may also be either one of them.[1] It is a soluble dye used as a biological dye,[2] in fluorescence microscopy, appearing a yellow-green colour after excitation with violet light.[3] It is a mixture of the trisulfonates of triphenyl rosaniline and of diphenyl rosaniline.[4]

Aniline blue or its constituents are used to stain collagen, as the fibre stain in Masson's trichrome,[5] as well as to reveal callose structures in plant tissues.[6]

It can be used in the Mallory's connective tissue stain[7] and Gömöri trichrome stain. It is used in differential staining.

References

  1. ↑ http://stainsfile.info/StainsFile/dyes/707.htm
  2. ↑ http://www.merriam-webster.com/medical/aniline%20blue
  3. ↑ http://www.biologie.uni-hamburg.de/b-online/library/webb/BOT410/410Labs/Fluor-99/fluorescence_microscope_images.htm
  4. ↑ http://medical-dictionary.thefreedictionary.com/aniline+blue
  5. ↑ http://stainsfile.info/StainsFile/dyes/42755.htm
  6. ↑ http://commonweb.unifr.ch/biol/pub/mauchgroup/staining.html
  7. ↑ http://stainsfile.info/StainsFile/dyes/42755.htm
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