Acid fuchsin

Acid fuchsin
Names

IUPAC name Disodium 2-amino-5-[(Z)-(4-amino-3-sulfonatophenyl)(4-iminio-3-sulfonato-2,5-cyclohexadien-1-ylidene)methyl]-3-methylbenzenesulfonate
Identifiers
CAS Number	3244-88-0
ChEBI	CHEBI:87052^Y
ChemSpider	16736101
Jmol interactive 3D	Image
InChI InChI=1S/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,21H,22-23H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2+1/p-2/b19-11-,21-14?;; Key: RZUBARUFLYGOGC-MTHOTQAESA-L InChI=1/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,21H,22-23H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2+1/p-2/b19-11-,21-14?;; Key: RZUBARUFLYGOGC-LOQRZINWBG
SMILES CC1=CC(=CC(=C1N)S(=O)(=O)[O-])/C(=C\2/C=CC(=[NH2+])C(=C2)S(=O)(=O)[O-])/C3=CC(=C(C=C3)N)S(=O)(=O)[O-].[Na+].[Na+]
Properties
Chemical formula	C₂₀H₁₇N₃Na₂O₉S₃
Molar mass	585.53 g·mol⁻¹
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Acid fuchsin is an acidic dye that has wide use in histology. It is one of the dyes used in Masson's trichrome stain. This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen. The muscle stains red with the acid fuchsin, and the collagen is stained green or blue with light green SF yellowish or methyl blue.

Formula:

1 g of acid fuchsin
1 mL of glacial acetic acid (HOAc)
100 mL of distilled water

References

Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974. ISBN 971-11-0853-4

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Acid fuchsin

See also

References