YdaO/yuaA leader

ydaO/yuaA leader
Predicted secondary structure and sequence conservation of ydaO-yuaA
Identifiers
Symbol ydaO-yuaA
Rfam RF00379
Other data
RNA type Cis-reg
Domain(s) Bacteria
SO 0000233

The YdaO/YuaA leader is a conserved RNA structure found upstream of the ydaO and yuaA genes in Bacillus subtilis and related genes in other bacteria. The ydaO/yuaA element is thought to act as a genetic "off" switch for the ydaO and yuaA genes in its native state. It is believed that the element may be triggered during osmotic shock leading to activation of ydaO, a predicted amino acid transporter gene, and members of the yuaA-yubG operon which code for KtrA and KtrB K+ transporters.[1]

Function

Further work has established that the ydaO element is widespread and is associated with a diverse set of genes which it controls in a sequence and structure-dependent fashion. The natural ligand is present in cells grown a defined media and transposon-directed knockouts result in disregulation of associated genes.[2]

A biochemical screen in Bacillus subtilis has shown that the ydaO motif specifically binds ATP and regulates the expression of genes in response to ATP. Thus demonstrating a role regulation of gene expression in response to energy balance in bacteria.[3]

References

  1. ↑ Barrick, JE; Corbino KA, Winkler WC, Nahvi A, Mandal M, Collins J, Lee M, Roth A, Sudarsan N, Jona I, Wickiser JK, Breaker RR (2004). "New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control". Proc Natl Acad Sci USA 101 (17): 6421–6426. doi:10.1073/pnas.0308014101. PMC 404060. PMID 15096624.
  2. ↑ Block KF, Hammond MC, Breaker RR (2010). "Evidence for Widespread Gene Control Function by the ydaO Riboswitch Candidate". J Bacteriol 192 (15): 3983–9. doi:10.1128/JB.00450-10. PMC 2916388. PMID 20511502.
  3. ↑ Watson PY, Fedor MJ (2012). "The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis.". Nat Chem Biol 8 (12): 963–5. doi:10.1038/nchembio.1095. PMID 23086297.

External links